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Kolorektal Kanserde Tiyol–Disülfit Düzeyleri

Year 2019, Volume: 24 Issue: 2, 137 - 141, 13.06.2019
https://doi.org/10.21673/anadoluklin.469201

Abstract

Amaç: Bu çalışmada kolorektal kanserli hastalarda natif tiyol, disülfit
ve total tiyol konsantrasyonları ile CEA ve CA-19-9 serum biyobelirteçlerini değerlendirmek
ve sonuçları sağlıklı bireylerle karşılaştırmak amaçlanmıştır.

Gereç ve Yöntemler: Çalışmaya 23 kolorektal kanserli hasta ve 23 sağlıklı
birey olmak üzere toplam 46 kişi dahil edildi. Tiyol/disülfit homeostaz
testleri (natif tiyol [-SH], disülfit [-S-S] ve total tiyol [TT]) yeni bir
otomatize metotla gerçekleştirildi. İskemi modifiye albümin (IMA), albümin,
karsinoembriyojenik antijen (CEA), karbonhidrat antijen 19-9 (CA-19-9), TT, -SH
ve -S-S seviyeleri ile disülfit/natif tiyol ve disülfit/total tiyol oranları
gruplar arasında karşılaştırıldı.

Bulgular: Kolorektal kanserli grupta IMA, CEA ve CA-19-9’da kontrol
grubuna kıyasla istatistiksel ola­rak anlamlı farklılık bulundu (p<0,05).
Kontrol grubunda tiyol–disülfit parametreleri ve tümör biyobelir­teçleri arasında
bir ilişki saptanmadı (p>0,05). Kolorektal kanserli hastalarda tiyol–disülfit
parametre­leri ile IMA, albümin, CEA ve CA-19-9 düzeyleri arasında bir ilişki
saptanmadı (p>0,05).







Tartışma ve Sonuç: Çalışmamız noninvaziv bir biyobelirteç olarak natif
tiyol (-SH), disülfit (-S-S) ve total tiyol (TT) konsantrasyonları ile
kolorektal kanser arasında bir ilişki olmadığını göstermektedir.

References

  • Referans1.Valko M, Rhodes CJ, Moncola J, et al. Free radicals, metals and antioxidants in oxidativestress-induced cancer.Chemico-Biological Interactions 160 ; 2006: 1–40.
  • Referans2. Özgönül A, Aksoy N, Dilmeç F, et al. Measurement of total antioxidant response in colorectal cancer using a novel automated method.Turk J Med Sci. 2009; 39 (4): 503-506.
  • Referans3. Türkiye İstatistik Kurumu, Ölüm Nedeni İstatistikleri, Haber Bülteni, 2017; Sayı: 27620. http://www.tuik.gov.tr/PreHaberBultenleri.do?id=27620
  • Referans4. Tatar M, Tatar F. “Colorectal cancer in Turkey: current situation and challenges for the future” Eur J Health Econ . 2010; 10 (Suppl 1):99–105.
  • Referans5. Bray F, Jemal A, Grey N, et al. Global cancer transitions according to the Human Development Index (2008–2030): a population-based study- The lancet oncology,13( 8): 790-801, 2012.
  • Referans6. Siegel RL, Miller KD, Jemal A. Cancer statistics, CA: A Cancer Journal for Clinicians 2017;67(1): 7-30.
  • Referans7. Özdemir A, Erel O. Thıol-Dısulfide Balance : Could be a new marker for Thyroid Cancer? Journal of Cancer Therapy (JCT). 2018; 9 (8).
  • Referans8. Skrzydlewska E, Sulkowski S,Koda M, Zalewski B, et al. Lipid peroxidation and antioxidant status in colorectal cancer. World Journal of Gastroenterology 2005;11(3):403-406.
  • Referans9. Skrzydlewska E, Kozuszko B, Sulkowska M, et al. Antioxidant potential in esophageal, stomach and colorectal cancers. Hepatogastroenterology 2003; 50(49): 126-31.
  • Referans10. Li F, Yang XW, Krausz KW, Nichols RG, et al. Modulation of Colon Cancer. Nutmeg J. Proteome Res. 2015;14: 1937−1946.
  • Referans11. Sedef Hande Enfez. Kemoterapinin Kolon-Kanseri Ve Meme-KanserindeVEGF Yüzeylerinde İnvivo Ve İnvitro İncelenmesi, Yüksek Lisans Tezi, Ankara Üniversitesi Biyoteknoloji Enstitüsü, 2010.
  • Referans12. Giustarini D, Dalle-Donne I, Lorenzini S, et al. Age-Related Influence on Thiol, Disulfide, and Protein-Mixed Disulfide Levels in Human Plasma.The Journals of Gerontology: Series A,61(10): 1030–1038, 2016. Referans13. BelenichevIF, Gorbacheva SV, Demchenko AV. Et al. The thiol-disulfide balance and the nitric oxide system in the brain tissue of rats subjected to experimental acute impairment of cerebral blood flow: The therapeutic effects of nootropic drugs. Neurochemical Journal, 2014; 8( 1): 24–27.
  • Referans14. Sedlak J, Lindsay RH. Estimation of total, proteinbound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem 1968; 25(1): 192-205.
  • Referans15. Davies MJ, Fu S, Wang H, et al. Stable markers of oxidant damage to proteins and their application in the study of human disease. Free Radic Biol Med 1999; 27(11-12): 1151-63.
  • Referans16. Cerutti PA. 1985, Prooxidant states and tumor promotion. Science; 227:375-81.
  • Referans17. Link A, Balaguer F, Shen Y, et al. Fecal MicroRNAs as Novel Biomarkers for Colon Cancer Screening. Cancer Epidemiol Biomarkers Prev; 19(7) July 2010.
  • Referans18. Ogata-Kawata H, Izumiya M, Kurioka D. et al. Circulating Exosomal microRNAs as Biomarkers of Colon Cancer. 2014:9 (4): e92921.
  • Referans19. Erel, O. and Neselioglu, SA. Novel and Automated Assay for Thiol/Disulfide Homeostasis. Clinical Biochemistry 2014; 47, 326-332. Referans20. Chianeh,YR., Prabhu,K. 2014, Protein Thiols as an Indicator of Oxidative Stress Oksidatif, Archives Medical Review Journal. 2014; 23(3): 443-456.Referans21. Dirican N, Dirican A, Şen O, Aynalı A, et al. Thiol/disulfide homeostasis: A prognostic biomarker for patients with advanced non-small cell lung cancer? Redox Report 2016; 21(5).
  • Referans22. Hizal M, Sendur MAN, Bilgin B. et al. Evaluation of dynamic serum thiol/disulfide homeostasis in locally advanced and metastatic gastric cancer. Journal of Oncological Sciences 4(2018) 1-4.

The Level of Thiol-Disulfide on Colorectal Cancer

Year 2019, Volume: 24 Issue: 2, 137 - 141, 13.06.2019
https://doi.org/10.21673/anadoluklin.469201

Abstract

Aim: This study aimed to evaluate total thiol (TT), disulfide (-S-S),
and native thiol (-SH) concentra­tions as serum biomarkers in patients with
colorectal cancer (CRC).

Materials and Methods: A total of 46 participants (23 patients with
colorectal cancer and 23 healthy individuals) were included. Thiol/disulfide
homeostasis tests (total thiol [TT], native thiol [-SH], and di­sulfide [-S-S])
were performed by a novel automated method. Ischemia modified albumin (IMA),
albu­min, carcinoembryonic antigen (CEA), carbohydrate antigen 19-9 (CA-19-9),
TT, -SH, and -S-S levels as well as disulfide/native thiol and disulfide/total
thiol ratios were compared between the groups.

Results: In the colorectal cancer group, statistically significant difference
was found in IMA, CEA, and CA-19-9, compared to the control group (p<0.05).
There was no relationship between the thiol–disul­fide parameters and tumor
markers in the control group (p>0.05). There was no relationship between the
thiol–disulfide parameters and IMA, albumin, CEA, and CA-19-9 levels in the
colorectal cancer group (p>0.05).







Discussion and Conclusion: Our study shows that the serum concentrations
of native -SH, -S-S, and TT do not link to colorectal cancer as a noninvasive
biomarker.

References

  • Referans1.Valko M, Rhodes CJ, Moncola J, et al. Free radicals, metals and antioxidants in oxidativestress-induced cancer.Chemico-Biological Interactions 160 ; 2006: 1–40.
  • Referans2. Özgönül A, Aksoy N, Dilmeç F, et al. Measurement of total antioxidant response in colorectal cancer using a novel automated method.Turk J Med Sci. 2009; 39 (4): 503-506.
  • Referans3. Türkiye İstatistik Kurumu, Ölüm Nedeni İstatistikleri, Haber Bülteni, 2017; Sayı: 27620. http://www.tuik.gov.tr/PreHaberBultenleri.do?id=27620
  • Referans4. Tatar M, Tatar F. “Colorectal cancer in Turkey: current situation and challenges for the future” Eur J Health Econ . 2010; 10 (Suppl 1):99–105.
  • Referans5. Bray F, Jemal A, Grey N, et al. Global cancer transitions according to the Human Development Index (2008–2030): a population-based study- The lancet oncology,13( 8): 790-801, 2012.
  • Referans6. Siegel RL, Miller KD, Jemal A. Cancer statistics, CA: A Cancer Journal for Clinicians 2017;67(1): 7-30.
  • Referans7. Özdemir A, Erel O. Thıol-Dısulfide Balance : Could be a new marker for Thyroid Cancer? Journal of Cancer Therapy (JCT). 2018; 9 (8).
  • Referans8. Skrzydlewska E, Sulkowski S,Koda M, Zalewski B, et al. Lipid peroxidation and antioxidant status in colorectal cancer. World Journal of Gastroenterology 2005;11(3):403-406.
  • Referans9. Skrzydlewska E, Kozuszko B, Sulkowska M, et al. Antioxidant potential in esophageal, stomach and colorectal cancers. Hepatogastroenterology 2003; 50(49): 126-31.
  • Referans10. Li F, Yang XW, Krausz KW, Nichols RG, et al. Modulation of Colon Cancer. Nutmeg J. Proteome Res. 2015;14: 1937−1946.
  • Referans11. Sedef Hande Enfez. Kemoterapinin Kolon-Kanseri Ve Meme-KanserindeVEGF Yüzeylerinde İnvivo Ve İnvitro İncelenmesi, Yüksek Lisans Tezi, Ankara Üniversitesi Biyoteknoloji Enstitüsü, 2010.
  • Referans12. Giustarini D, Dalle-Donne I, Lorenzini S, et al. Age-Related Influence on Thiol, Disulfide, and Protein-Mixed Disulfide Levels in Human Plasma.The Journals of Gerontology: Series A,61(10): 1030–1038, 2016. Referans13. BelenichevIF, Gorbacheva SV, Demchenko AV. Et al. The thiol-disulfide balance and the nitric oxide system in the brain tissue of rats subjected to experimental acute impairment of cerebral blood flow: The therapeutic effects of nootropic drugs. Neurochemical Journal, 2014; 8( 1): 24–27.
  • Referans14. Sedlak J, Lindsay RH. Estimation of total, proteinbound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem 1968; 25(1): 192-205.
  • Referans15. Davies MJ, Fu S, Wang H, et al. Stable markers of oxidant damage to proteins and their application in the study of human disease. Free Radic Biol Med 1999; 27(11-12): 1151-63.
  • Referans16. Cerutti PA. 1985, Prooxidant states and tumor promotion. Science; 227:375-81.
  • Referans17. Link A, Balaguer F, Shen Y, et al. Fecal MicroRNAs as Novel Biomarkers for Colon Cancer Screening. Cancer Epidemiol Biomarkers Prev; 19(7) July 2010.
  • Referans18. Ogata-Kawata H, Izumiya M, Kurioka D. et al. Circulating Exosomal microRNAs as Biomarkers of Colon Cancer. 2014:9 (4): e92921.
  • Referans19. Erel, O. and Neselioglu, SA. Novel and Automated Assay for Thiol/Disulfide Homeostasis. Clinical Biochemistry 2014; 47, 326-332. Referans20. Chianeh,YR., Prabhu,K. 2014, Protein Thiols as an Indicator of Oxidative Stress Oksidatif, Archives Medical Review Journal. 2014; 23(3): 443-456.Referans21. Dirican N, Dirican A, Şen O, Aynalı A, et al. Thiol/disulfide homeostasis: A prognostic biomarker for patients with advanced non-small cell lung cancer? Redox Report 2016; 21(5).
  • Referans22. Hizal M, Sendur MAN, Bilgin B. et al. Evaluation of dynamic serum thiol/disulfide homeostasis in locally advanced and metastatic gastric cancer. Journal of Oncological Sciences 4(2018) 1-4.
There are 19 citations in total.

Details

Primary Language English
Journal Section ORIGINAL ARTICLE
Authors

Ayşe Özdemir 0000-0003-2639-7344

Utku Dönem Dilli This is me

Dalyan Özdemir This is me 0000-0003-2639-7344

Salim Neşelioğlu

Özcan Erel

Publication Date June 13, 2019
Acceptance Date January 11, 2019
Published in Issue Year 2019 Volume: 24 Issue: 2

Cite

Vancouver Özdemir A, Dilli UD, Özdemir D, Neşelioğlu S, Erel Ö. The Level of Thiol-Disulfide on Colorectal Cancer. Anatolian Clin. 2019;24(2):137-41.

13151 This Journal licensed under a CC BY-NC (Creative Commons Attribution-NonCommercial 4.0) International License.