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Total fertilization failure in intracytoplasmic sperm injection: a retrospective comparative study

Year 2023, Volume: 48 Issue: 1, 168 - 176, 31.03.2023
https://doi.org/10.17826/cumj.1221276

Abstract

Purpose: The aim of this study was to evaluate total fertilization failure (TFF), embryo transfers following TFF cycles, and recurrent TFF, and to define the factors that might cause TFF.
Materials and Methods: Cycles that resulted in TFF (group 1, n=109), embryo transfers after TFF cycles (group 2, n=30), and cases of recurrent TFF (group 3, n=15) were evaluated retrospectively.
Results: Peak estradiol was higher in group 2 than other groups, and the rate of sperm morphology below 4% was lower in group 2 when compared to groups 1 and 3. The total numbers of retrieved oocytes (5.3±4.1 vs 10.2±1.5 (Odds Ratio=1.639; 95% CI 1.267-2.122)) and MII oocytes (2.9±2.2 vs 6.8±1.8 (Odds Ratio=2.218; 95% CI 1.529-3.216)) were significantly higher in embryo transfer cycles when compared to previous TFF cycles. Retrieved and MII oocytes counts were higher in group 2 with a median fertilization rate of 46.42% when compared to groups 1 and 3.
Conclusion: With more retrieved and MII oocytes, normal sperm morphology can increase the fertilization rate of ICSI cycles following earlier TFF.

References

  • Inal HA, Kahyaoglu I, Turkkani A, Tuzluoglu D, Yilmaz N. Retrospective comparison of intracytoplasmic sperm injection outcomes of sperm retrieved from a testicular biopsy and freshly ejaculated semen in oligozoospermia. Rev Int Androl. 2018;16:131-6.
  • Kahyaoglu I, Demir B, Turkkanı A, Cınar O, Dilbaz S, Dilbaz B, Mollamahmutoglu L. Total fertilization failure: is it the end of the story? J Assist Reprod Genet. 2014;31:1155-60.
  • Goksan Pabuccu E, Sinem Caglar G, Dogus Demirkiran O, Pabuccu R. Uncommon but devastating event: total fertilisation failure following intracytoplasmic sperm injection. Andrologia. 2016;48:164-70.
  • Inal ZO, Inal HA, Gorkem U. Sexual function and depressive symptoms in primary infertile women with vitamin D deficiency undergoing IVF treatment. Taiwan J Obstet Gynecol. 2020;59:91-8.
  • Yilmaz N, Kahyaoglu İ, İnal HA, Görkem Ü, Devran A, Mollamahmutoglu L. Negative life events have detrimental effects on in-vitro fertlization outcome. Hum Fertil (Camb). 2015;18:220-4.
  • Inal HA, Ozturk Inal Z, Mermer S, Aksoy E, Bayraktar AM, Can U. Investigation of serum vitamin D and ischaemia-modified albumin levels in infertile Turkish men. Andrologia. 2020;52:e13507.
  • Sfontouris IA, Nastri CO, Lima ML, Tahmasbpourmarzouni E, Raine-Fenning N, Martins WP. Artificial oocyte activation to improve reproductive outcomes in women with previous fertilization failure: a systematic review and meta-analysis of RCTs. Hum Reprod. 2015;30:1831-41.
  • Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology. The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. Hum Reprod. 2011;26:1270-83.
  • Bonte D, Ferrer-Buitrago M, Dhaenens L, Popovic M, Thys V, De Croo I et al. Assisted oocyte activation significantly increases fertilization and pregnancy outcome in patients with low and total failed fertilization after intracytoplasmic sperm injection: a 17-year retrospective study. Fertil Steril. 2019;112:266-74.
  • Gross KX, Hotaling JM. Improving outcomes of after fertilization failure with intracytoplasmic sperm injection: the role of differentiating oocyte-related and sperm-related oocyte activation deficiency. Fertil Steril. 2020;114:497.
  • Cassuto NG, Bouret D, Plouchart JM, Jellad S, Vanderzwalmen P, Balet R et al. A new real-time morphology classification for human spermatozoa: a link for fertilization and improved embryo quality. Fertil Steril. 2009;92:1616–25.
  • Inal ZO, Inal HA, Aksoy E, Kucukkendirci H. Spermiogram test results of patients presenting to our IVF Center due to infertility. J Clin Anal Med 2017;8:365-9.
  • Ebner T, Shebl O, Oppelt P, Mayer RB. Some reflections on intracytoplasmic morphologically selected sperm injection. Int J Fertil Steril. 2014;8:105-12.
  • Melie NA, Adeniyi OA, Igbineweka OM, Ajayi RA. Predictive value of the number of oocytes retrieved at ultrasound-directed follicular aspiration with regard to fertilization rates and pregnancy outcome in intracytoplasmic sperm injection treatment cycles. Fertil Steril. 2003;80:1376–9.
  • Xia P. Intracytoplasmic sperm injection: correlation of oocyte grade based on polar body, perivitelline space and cytoplasmic inclusions with fertilization rate and embryo quality. Hum Reprod. 1997;12:1750–5.
  • Ferrer-Buitrago M, Bonte D, De Sutter P, Leybaert L, Heindryckx B. Single Ca2+ transients vs oscillatory Ca2+ signaling for assisted oocyte activation: limitations and benefits. Reproduction. 2018;155:105-19.
  • Polyzos NP, Blockeel C, Verpoest W, De Vos M, Stoop D, Vloeberghs V et al. Live birth rates following natural cycle IVF in women with poor ovarian response according to the Bologna criteria. Hum Reprod. 2012;27:3481–6.

İntrasitoplazmik sperm enjeksiyonunda döllenme başarısızlığı: retrospektif karşılaştırmalı bir çalışma

Year 2023, Volume: 48 Issue: 1, 168 - 176, 31.03.2023
https://doi.org/10.17826/cumj.1221276

Abstract

Amaç: Bu çalışmada total fertilizasyon başarısızlığı (TFF), TFF siklusları sonrası embriyo transferleri ve tekrarlayan TFF'yi değerlendirmek ve TFF'ye neden olabilecek faktörlerinin belirlenmesi amaçlanmıştır.
Gereç ve Yöntem: TFF ile sonuçlanan sikluslar (grup 1, n=109), TFF siklusları sonrası embriyo transferleri (grup 2, n=30) ve tekrarlayan TFF olguları (grup 3, n=15) retrospektif olarak değerlendirildi.
Bulgular: Pik estradiol grup 2'de diğer gruplara göre daha yüksekti, %4'ün altında sperm morfolojisi oranı grup 2'de grup 1 ve 3'e göre daha düşüktü. Elde edilen toplam oosit sayısı (5,3±4,1 vs 10,2±1,5 (Odds Ratio=1,639; %95 CI 1,267-2,122) ve MII oositleri (2,9±2,2 vs 6,8±1,8 (Odds Ratio=2,218; 95) % CI 1,529-3,216;) embriyo transfer sikluslarında önceki TFF sikluslarına göre anlamlı derecede yüksekti. Elde edilen ve MII oosit sayısı medyan fertilizasyon oranı %46.42 ile grup 1 ve 3'e göre grup 2'de daha yüksekti.
Sonuç: Daha fazla toplanan ve MII oositlerle, normal sperm morfolojisi, erken TFF'yi takiben ICSI sikluslarının fertilizasyon oranını artırabilir.

References

  • Inal HA, Kahyaoglu I, Turkkani A, Tuzluoglu D, Yilmaz N. Retrospective comparison of intracytoplasmic sperm injection outcomes of sperm retrieved from a testicular biopsy and freshly ejaculated semen in oligozoospermia. Rev Int Androl. 2018;16:131-6.
  • Kahyaoglu I, Demir B, Turkkanı A, Cınar O, Dilbaz S, Dilbaz B, Mollamahmutoglu L. Total fertilization failure: is it the end of the story? J Assist Reprod Genet. 2014;31:1155-60.
  • Goksan Pabuccu E, Sinem Caglar G, Dogus Demirkiran O, Pabuccu R. Uncommon but devastating event: total fertilisation failure following intracytoplasmic sperm injection. Andrologia. 2016;48:164-70.
  • Inal ZO, Inal HA, Gorkem U. Sexual function and depressive symptoms in primary infertile women with vitamin D deficiency undergoing IVF treatment. Taiwan J Obstet Gynecol. 2020;59:91-8.
  • Yilmaz N, Kahyaoglu İ, İnal HA, Görkem Ü, Devran A, Mollamahmutoglu L. Negative life events have detrimental effects on in-vitro fertlization outcome. Hum Fertil (Camb). 2015;18:220-4.
  • Inal HA, Ozturk Inal Z, Mermer S, Aksoy E, Bayraktar AM, Can U. Investigation of serum vitamin D and ischaemia-modified albumin levels in infertile Turkish men. Andrologia. 2020;52:e13507.
  • Sfontouris IA, Nastri CO, Lima ML, Tahmasbpourmarzouni E, Raine-Fenning N, Martins WP. Artificial oocyte activation to improve reproductive outcomes in women with previous fertilization failure: a systematic review and meta-analysis of RCTs. Hum Reprod. 2015;30:1831-41.
  • Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology. The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. Hum Reprod. 2011;26:1270-83.
  • Bonte D, Ferrer-Buitrago M, Dhaenens L, Popovic M, Thys V, De Croo I et al. Assisted oocyte activation significantly increases fertilization and pregnancy outcome in patients with low and total failed fertilization after intracytoplasmic sperm injection: a 17-year retrospective study. Fertil Steril. 2019;112:266-74.
  • Gross KX, Hotaling JM. Improving outcomes of after fertilization failure with intracytoplasmic sperm injection: the role of differentiating oocyte-related and sperm-related oocyte activation deficiency. Fertil Steril. 2020;114:497.
  • Cassuto NG, Bouret D, Plouchart JM, Jellad S, Vanderzwalmen P, Balet R et al. A new real-time morphology classification for human spermatozoa: a link for fertilization and improved embryo quality. Fertil Steril. 2009;92:1616–25.
  • Inal ZO, Inal HA, Aksoy E, Kucukkendirci H. Spermiogram test results of patients presenting to our IVF Center due to infertility. J Clin Anal Med 2017;8:365-9.
  • Ebner T, Shebl O, Oppelt P, Mayer RB. Some reflections on intracytoplasmic morphologically selected sperm injection. Int J Fertil Steril. 2014;8:105-12.
  • Melie NA, Adeniyi OA, Igbineweka OM, Ajayi RA. Predictive value of the number of oocytes retrieved at ultrasound-directed follicular aspiration with regard to fertilization rates and pregnancy outcome in intracytoplasmic sperm injection treatment cycles. Fertil Steril. 2003;80:1376–9.
  • Xia P. Intracytoplasmic sperm injection: correlation of oocyte grade based on polar body, perivitelline space and cytoplasmic inclusions with fertilization rate and embryo quality. Hum Reprod. 1997;12:1750–5.
  • Ferrer-Buitrago M, Bonte D, De Sutter P, Leybaert L, Heindryckx B. Single Ca2+ transients vs oscillatory Ca2+ signaling for assisted oocyte activation: limitations and benefits. Reproduction. 2018;155:105-19.
  • Polyzos NP, Blockeel C, Verpoest W, De Vos M, Stoop D, Vloeberghs V et al. Live birth rates following natural cycle IVF in women with poor ovarian response according to the Bologna criteria. Hum Reprod. 2012;27:3481–6.
There are 17 citations in total.

Details

Primary Language English
Subjects Clinical Sciences
Journal Section Research
Authors

Hasan İnal 0000-0002-8361-7908

Zeynep Ozturk Inal 0000-0002-8766-2079

Publication Date March 31, 2023
Acceptance Date February 1, 2023
Published in Issue Year 2023 Volume: 48 Issue: 1

Cite

MLA İnal, Hasan and Zeynep Ozturk Inal. “Total Fertilization Failure in Intracytoplasmic Sperm Injection: A Retrospective Comparative Study”. Cukurova Medical Journal, vol. 48, no. 1, 2023, pp. 168-76, doi:10.17826/cumj.1221276.