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Molecular epidemiology of Blastocystis

Year 2015, Volume: 42 Issue: 4, 541 - 546, 08.01.2016

Fadime Eroğlu

Abstract

Blastocystis pathogenicity and classification was newly illuminated with molecular genetic studies and recently the parasite was found in the focus of many researchers. Several molecular methods such as; polymerase chain reaction (PCR), PCR-restriction fragment length polymorphism, random amplified polymorphic DNA, real-time polymerase chain reaction and DNA sequencing analyses can be used in genotyping of Blastocystis. Blastocystis parasites may cause diarrhea, abdominal pain, bloating, gas, irritability, anorexia, cramps, vomiting, dehydration, insomnia, nausea, loss of appetite, weight loss, fatigue symptoms and also could be asymptomatic cases. In this review, it was aimed to summarize the associations between Blastocystis subtypes and pathogenicity.

Key words: Blastocystis, subtype, pathogenicity

Keywords

Blastocystis alt tip patojenite

References

  • Yapıcı F, Sönmez Tamer G, Arısoy ES. Çoçuklarda bağırsak parazitlerinin dağılımı ve bununla ilişkili etmenler. Turkiye Parazitol Derg 2008;32:346-350.
  • Kurt Ö. Blastosistis: Dünya’daki son çalışmalar üzerinden geleceğe
  • bakış. 18.Ulusal Parazitoloji Kongresi; 29 Eylül-5 Ekim; Denizli-Türkiye 2013;31.
  • Pektaş B, Gökmen AA, İnci A, et al. Bir eğitim araştırma hastanesinde üç yıllık bağırsak parazitlerinin dağılımı: retrospektif
  • bir çalışma. J Clin Exp Invest 2015;6:269-273.
  • Alfellani MA, Stensvold CR, Vidal-Lapiedra A, et al. Variable geographic distribution of Blastocystis subtypes and its potential
  • implications. Acta Trop 2013;126:11-18.
  • Ertuğ S, Malatyalı E, Ertabaklar H, et al. Aydın ilinde elde edilen Blastocystis izolatlarının alt tip dağılımı ve klinik semptomların değerlendirilmesi. Microbiyol Bul 2015;49:98-104.
  • Tan KS. New insights on classification, identification, and clinical relevance of Blastocystis spp. Clin Microbiol Rev 2008;21:639-665.
  • Malatyalı E, Ozçelik S. Blastocystis spp.’nin insandan izolasyonu
  • ve besiyerinde farklı evrim şekillerinin izlenmesi. Turkiye Parazitol Derg 2011;35:19-22.
  • Dogruman Al F. Blastosistis enfeksiyonlarının günümüzde ilişkilendirildiği klinik tablolar, bağırsak mikrobiyon projesinin
  • getirdikleri 18. Ulusal Parazitoloji Kongresi DenizliTürkiye 2013;32.
  • Adıyaman Korkmaz G, Dogruman Al F, et al. Dışkı örneklerinde
  • Blastocystis spp. varlığının mikroskobik, kültür ve moleküler yöntemlerle araştırılması. Microbiyol Bul 2015;49:85-97.
  • Stenzel DJ, Boreham PF. Blastocystis hominis revisited. Clinical
  • Microbiol Rev 1996;9:563-584.
  • Silberman JD, Sogin ML, Leipe DD, et al. Human parasite finds taxonomic home. Nature 1996;4:380(6573):398.
  • İnceboz T, Usluca S. Blastocystis hominis bağırsak hastalığı için potansiyel bir tehlike olabilir mi? DEÜ Tıp Fak Derg 2009;23:37-45.
  • Nagel R, Traub RJ, Kwan M, et al. Blastocystis specific serum
  • immunoglobulin in patients with irritable bowel sydrome (IBS) versus healthy controls. Parasit Vectors 2015;15:8:453.
  • Stensvold CR. Blastocystis: genetic diversity and molecular
  • methods for diagnosis and epidemiology. Trop Parasitol 2013;3:26-34.
  • https://tr.wikipedia.org/wiki/polimeraz_zincir_tepkimesi
  • Yoshikawa H, Wu Z, Kımata I, et al. Polymerase chain reactionbased genotype classification among human Blastocystis
  • hominis populations isolated from different countries. Parasitol
  • Res 2004;92:22-29.
  • Öz Aydın S. RAPD (Rastgele arttırılmış polimorfik DNA elirleyicileri
  • ve bitki sistematiği) Dumlupınar Üniversitesi Fen Bilimleri Enstitüsü Dergisi 2004:113-130.
  • Yoshikawa H, Nagano I, Wu Z, et al. Genomic polymorphism among Blastocystis hominis strains and development of subtype-pecific diagnostic primers. Mol Cell Probes 1998;153-159.
  • www.ncbi.nlm.nih.gov/probe/docs/techrflp
  • Hoevers J, Holman P, Logan K, et al. Restriction fragmentlength
  • polymorphism analysis of small-subunit rRNA genes of Blastocystis hominis isolates from geographically diverse human hosts. Parasitol Res 2000;86:57-61.
  • Abe N, Wu Z, Yoshikawa H. Zoonotic genotypes of Blastocystis
  • hominis detected in cattle and pigs by PCR with diagnostic primers and restriction fragment length polymorphisms analysis of the small subunit ribosomal RNA gene. Parasitol Res 2003;90:124-128.
  • Clark CG. Extensive genetic diversity in Blastocystis hominis.
  • Mol Biochem Parasitol 1997;87:79-83.
  • Jones MS2nd, Ganac RD, Hiser G, et al. Detection of Blastocystis
  • from stool samples using real-time PCR. Parasitol Res 2008;103:551-557.
  • Stensvold CR, Ahmed UN, Andersen L, et al. Development and evalutaion of a genus-specific, probe-based, internal-process-ontrolled real-time PCR assay for sensitive and specific detection of Blastocystis spp. J Clin Microbiol 2012;50:1847-1851.
  • ww.deu.edu.tr/uplodedFiles/Birimler/16928/dna_dizi_analizi.pdf
  • Scicluna SM, Tawari B, Clark CG. DNA barcoding of Blastocystis.
  • Protist 2006;157:77-85.
  • Stensvold R, Brillowska-Dabrowska A, Nielsen HV, et al. Detection
  • of Blastocystis hominis in unpreserved stool specimens by using polymerase chain reaction. J Parasitol 2006;92:1081-1087.
  • Noel C, Peyronnet C, Gerbod D, et al. Phylogenetic analysis of
  • Blastocystis isolates from different hosts based on the comparison
  • of small-subunit RRNA gene sequences. Mol Biochem Parasitol 2003;126;119-123.
  • Casero RD, Mongi F, Sanchez A, et al. Blastocystis and urticaria:
  • examination of subtypes and morphotypes in an unusual clinical manifestation. Acta Trop 2015;148:156-161.
  • Wawrzniak I, Courtine D, Osman M, et al. Draft genome sequence of the intestinal parasite Blastocystis subtype 4-isolate
  • W11. Genomic Data 2015;4:22-23.
  • Mattiucci S, Crisafi B, Gabrielli S, et al. Molecular epidemiology
  • and genetic diversity of Blastocystis infection in humans in Italy. Epidemiol Infect 2015;21:1-2.
  • Ramirez JD, Sanchez LV, Bautista DC, et al. Blastocystis subtypes
  • detected in humans and animals from Colombia. Infect Genet Evol 2014;22:223-228.
  • Malheiros A, Stensvold C, Clark CG, et al. Molecular characterization of Blastocystis obtained from members of the indigenous Tapirape ethnic group from the Brazilian Amazon region,
  • Brazil. Am J Trop Med Hyg 2011;85:1050-1053.
  • Villalobos G, Orozco-Mosqueda G, Lopez-Perez M, et al. Suitability
  • of internal transcribed spacers (ITS) as markers for the population genetic structure of Blastocystis spp. Parasit Vectors 2014;7:461.
  • Badparva E, Sadraee J, Kheirandish F. Genetic diversity of Blastocystis isolated from cattle in Khorramabed, Iran. Indishapur
  • J Microbiol 2015;8:e14810.
  • Popruk S, Udonsom R, Koompapong K. Subtype distribution
  • of Blastocystis in Thai-Myanmar Border, Thailand. Korean J Parasitol 2015;53:13-19.
  • Abu-Madi M, Aly M, Behnke JM, et al. The distribution of Blastocystis subtypes in isolates from Qatar. Parasit Vectors 2015;8:405.
  • Dagci H, Kurt Ö, Demirel M, et al. Epidemiological and diagnostic
  • features of blastocystis infection in symptomatic patients in İzmir province, Turkey. Iran J Parasitol 2014;9:519-529.
  • Tasova Y, Sahin B, Koltas S, et al. Clinical significance and frequency of Blastocystis hominis in Turkish patients with
  • hematological malignancy. Acta Med Okayama 2000;54:133-136.
  • Yan Y, Su S, Lai R, et al. Genetic variability of Blastocystis hominis soaltes in China. Parasitol Res 2006;99:597-601.
  • Dominguez-Marquez MV, Guna R, Munoz C, et al. High prevalence of subtype 4 among isolates of Blastocystis hominis
  • from symptomatic patients of a health district of Valencia (Spain) Parasitol Res 2009;105:949-955.
  • Dogruman Al F, Dagcı H, Yoshikawa H, et al. A possible link between subtype 2 and asymptomatic infections of Blastocystis
  • hominis. Parasitol Res 2008;103:685-689.
  • Pektaş B, Yıldırım A, Gökmen AA, et al. İrritabl barsak sendromlu
  • hastalarda blastocystis hominis sıklığının araştırılması. J Clin Exp Invest 2014;5:242-245.
  • Casero RD, Mongi F, Sanchez A, et al. Blastocystis and urticaria:
  • examination of subtypes and morphotypes in an unusual clinical manifestation. Acta Trop 2015;148:156-161.

Year 2015, Volume: 42 Issue: 4, 541 - 546, 08.01.2016

Fadime Eroğlu

Abstract

References

  • Yapıcı F, Sönmez Tamer G, Arısoy ES. Çoçuklarda bağırsak parazitlerinin dağılımı ve bununla ilişkili etmenler. Turkiye Parazitol Derg 2008;32:346-350.
  • Kurt Ö. Blastosistis: Dünya’daki son çalışmalar üzerinden geleceğe
  • bakış. 18.Ulusal Parazitoloji Kongresi; 29 Eylül-5 Ekim; Denizli-Türkiye 2013;31.
  • Pektaş B, Gökmen AA, İnci A, et al. Bir eğitim araştırma hastanesinde üç yıllık bağırsak parazitlerinin dağılımı: retrospektif
  • bir çalışma. J Clin Exp Invest 2015;6:269-273.
  • Alfellani MA, Stensvold CR, Vidal-Lapiedra A, et al. Variable geographic distribution of Blastocystis subtypes and its potential
  • implications. Acta Trop 2013;126:11-18.
  • Ertuğ S, Malatyalı E, Ertabaklar H, et al. Aydın ilinde elde edilen Blastocystis izolatlarının alt tip dağılımı ve klinik semptomların değerlendirilmesi. Microbiyol Bul 2015;49:98-104.
  • Tan KS. New insights on classification, identification, and clinical relevance of Blastocystis spp. Clin Microbiol Rev 2008;21:639-665.
  • Malatyalı E, Ozçelik S. Blastocystis spp.’nin insandan izolasyonu
  • ve besiyerinde farklı evrim şekillerinin izlenmesi. Turkiye Parazitol Derg 2011;35:19-22.
  • Dogruman Al F. Blastosistis enfeksiyonlarının günümüzde ilişkilendirildiği klinik tablolar, bağırsak mikrobiyon projesinin
  • getirdikleri 18. Ulusal Parazitoloji Kongresi DenizliTürkiye 2013;32.
  • Adıyaman Korkmaz G, Dogruman Al F, et al. Dışkı örneklerinde
  • Blastocystis spp. varlığının mikroskobik, kültür ve moleküler yöntemlerle araştırılması. Microbiyol Bul 2015;49:85-97.
  • Stenzel DJ, Boreham PF. Blastocystis hominis revisited. Clinical
  • Microbiol Rev 1996;9:563-584.
  • Silberman JD, Sogin ML, Leipe DD, et al. Human parasite finds taxonomic home. Nature 1996;4:380(6573):398.
  • İnceboz T, Usluca S. Blastocystis hominis bağırsak hastalığı için potansiyel bir tehlike olabilir mi? DEÜ Tıp Fak Derg 2009;23:37-45.
  • Nagel R, Traub RJ, Kwan M, et al. Blastocystis specific serum
  • immunoglobulin in patients with irritable bowel sydrome (IBS) versus healthy controls. Parasit Vectors 2015;15:8:453.
  • Stensvold CR. Blastocystis: genetic diversity and molecular
  • methods for diagnosis and epidemiology. Trop Parasitol 2013;3:26-34.
  • https://tr.wikipedia.org/wiki/polimeraz_zincir_tepkimesi
  • Yoshikawa H, Wu Z, Kımata I, et al. Polymerase chain reactionbased genotype classification among human Blastocystis
  • hominis populations isolated from different countries. Parasitol
  • Res 2004;92:22-29.
  • Öz Aydın S. RAPD (Rastgele arttırılmış polimorfik DNA elirleyicileri
  • ve bitki sistematiği) Dumlupınar Üniversitesi Fen Bilimleri Enstitüsü Dergisi 2004:113-130.
  • Yoshikawa H, Nagano I, Wu Z, et al. Genomic polymorphism among Blastocystis hominis strains and development of subtype-pecific diagnostic primers. Mol Cell Probes 1998;153-159.
  • www.ncbi.nlm.nih.gov/probe/docs/techrflp
  • Hoevers J, Holman P, Logan K, et al. Restriction fragmentlength
  • polymorphism analysis of small-subunit rRNA genes of Blastocystis hominis isolates from geographically diverse human hosts. Parasitol Res 2000;86:57-61.
  • Abe N, Wu Z, Yoshikawa H. Zoonotic genotypes of Blastocystis
  • hominis detected in cattle and pigs by PCR with diagnostic primers and restriction fragment length polymorphisms analysis of the small subunit ribosomal RNA gene. Parasitol Res 2003;90:124-128.
  • Clark CG. Extensive genetic diversity in Blastocystis hominis.
  • Mol Biochem Parasitol 1997;87:79-83.
  • Jones MS2nd, Ganac RD, Hiser G, et al. Detection of Blastocystis
  • from stool samples using real-time PCR. Parasitol Res 2008;103:551-557.
  • Stensvold CR, Ahmed UN, Andersen L, et al. Development and evalutaion of a genus-specific, probe-based, internal-process-ontrolled real-time PCR assay for sensitive and specific detection of Blastocystis spp. J Clin Microbiol 2012;50:1847-1851.
  • ww.deu.edu.tr/uplodedFiles/Birimler/16928/dna_dizi_analizi.pdf
  • Scicluna SM, Tawari B, Clark CG. DNA barcoding of Blastocystis.
  • Protist 2006;157:77-85.
  • Stensvold R, Brillowska-Dabrowska A, Nielsen HV, et al. Detection
  • of Blastocystis hominis in unpreserved stool specimens by using polymerase chain reaction. J Parasitol 2006;92:1081-1087.
  • Noel C, Peyronnet C, Gerbod D, et al. Phylogenetic analysis of
  • Blastocystis isolates from different hosts based on the comparison
  • of small-subunit RRNA gene sequences. Mol Biochem Parasitol 2003;126;119-123.
  • Casero RD, Mongi F, Sanchez A, et al. Blastocystis and urticaria:
  • examination of subtypes and morphotypes in an unusual clinical manifestation. Acta Trop 2015;148:156-161.
  • Wawrzniak I, Courtine D, Osman M, et al. Draft genome sequence of the intestinal parasite Blastocystis subtype 4-isolate
  • W11. Genomic Data 2015;4:22-23.
  • Mattiucci S, Crisafi B, Gabrielli S, et al. Molecular epidemiology
  • and genetic diversity of Blastocystis infection in humans in Italy. Epidemiol Infect 2015;21:1-2.
  • Ramirez JD, Sanchez LV, Bautista DC, et al. Blastocystis subtypes
  • detected in humans and animals from Colombia. Infect Genet Evol 2014;22:223-228.
  • Malheiros A, Stensvold C, Clark CG, et al. Molecular characterization of Blastocystis obtained from members of the indigenous Tapirape ethnic group from the Brazilian Amazon region,
  • Brazil. Am J Trop Med Hyg 2011;85:1050-1053.
  • Villalobos G, Orozco-Mosqueda G, Lopez-Perez M, et al. Suitability
  • of internal transcribed spacers (ITS) as markers for the population genetic structure of Blastocystis spp. Parasit Vectors 2014;7:461.
  • Badparva E, Sadraee J, Kheirandish F. Genetic diversity of Blastocystis isolated from cattle in Khorramabed, Iran. Indishapur
  • J Microbiol 2015;8:e14810.
  • Popruk S, Udonsom R, Koompapong K. Subtype distribution
  • of Blastocystis in Thai-Myanmar Border, Thailand. Korean J Parasitol 2015;53:13-19.
  • Abu-Madi M, Aly M, Behnke JM, et al. The distribution of Blastocystis subtypes in isolates from Qatar. Parasit Vectors 2015;8:405.
  • Dagci H, Kurt Ö, Demirel M, et al. Epidemiological and diagnostic
  • features of blastocystis infection in symptomatic patients in İzmir province, Turkey. Iran J Parasitol 2014;9:519-529.
  • Tasova Y, Sahin B, Koltas S, et al. Clinical significance and frequency of Blastocystis hominis in Turkish patients with
  • hematological malignancy. Acta Med Okayama 2000;54:133-136.
  • Yan Y, Su S, Lai R, et al. Genetic variability of Blastocystis hominis soaltes in China. Parasitol Res 2006;99:597-601.
  • Dominguez-Marquez MV, Guna R, Munoz C, et al. High prevalence of subtype 4 among isolates of Blastocystis hominis
  • from symptomatic patients of a health district of Valencia (Spain) Parasitol Res 2009;105:949-955.
  • Dogruman Al F, Dagcı H, Yoshikawa H, et al. A possible link between subtype 2 and asymptomatic infections of Blastocystis
  • hominis. Parasitol Res 2008;103:685-689.
  • Pektaş B, Yıldırım A, Gökmen AA, et al. İrritabl barsak sendromlu
  • hastalarda blastocystis hominis sıklığının araştırılması. J Clin Exp Invest 2014;5:242-245.
  • Casero RD, Mongi F, Sanchez A, et al. Blastocystis and urticaria:
  • examination of subtypes and morphotypes in an unusual clinical manifestation. Acta Trop 2015;148:156-161.
There are 78 citations in total.

Details

Primary Language Turkish
Journal Section Research Articles
Authors

Fadime Eroğlu

Publication Date January 8, 2016
Submission Date January 8, 2016
Published in Issue Year 2015 Volume: 42 Issue: 4

Cite

APA Eroğlu, F. (2016). Molecular epidemiology of Blastocystis. Dicle Tıp Dergisi, 42(4), 541-546. https://doi.org/10.5798/diclemedj.0921.2015.04.0627
AMA Eroğlu F. Molecular epidemiology of Blastocystis. diclemedj. January 2016;42(4):541-546. doi:10.5798/diclemedj.0921.2015.04.0627
Chicago Eroğlu, Fadime. “Molecular Epidemiology of Blastocystis”. Dicle Tıp Dergisi 42, no. 4 (January 2016): 541-46. https://doi.org/10.5798/diclemedj.0921.2015.04.0627.
EndNote Eroğlu F (January 1, 2016) Molecular epidemiology of Blastocystis. Dicle Tıp Dergisi 42 4 541–546.
IEEE F. Eroğlu, “Molecular epidemiology of Blastocystis”, diclemedj, vol. 42, no. 4, pp. 541–546, 2016, doi: 10.5798/diclemedj.0921.2015.04.0627.
ISNAD Eroğlu, Fadime. “Molecular Epidemiology of Blastocystis”. Dicle Tıp Dergisi 42/4 (January 2016), 541-546. https://doi.org/10.5798/diclemedj.0921.2015.04.0627.
JAMA Eroğlu F. Molecular epidemiology of Blastocystis. diclemedj. 2016;42:541–546.
MLA Eroğlu, Fadime. “Molecular Epidemiology of Blastocystis”. Dicle Tıp Dergisi, vol. 42, no. 4, 2016, pp. 541-6, doi:10.5798/diclemedj.0921.2015.04.0627.
Vancouver Eroğlu F. Molecular epidemiology of Blastocystis. diclemedj. 2016;42(4):541-6.