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Evaluation on Antimicrobial Activity of The Lichen Pleurosticta acetabulum

Year 2023, Volume: 11 Issue: 1, 199 - 203, 31.01.2023
https://doi.org/10.29130/dubited.1082155

Abstract

In this study, the ethanol extract of the lichen Pleurosticta acetabulum (Neck.) Elix & Lumbsch (Parmeliaceae) was investigated for their antimicrobial activity against various microorganisms by the disc diffusion method. Mycobacterium smegmatis CCM 2067, Micrococcus luteus CCM 169, Staphylococcus aureus ATCC 6538P, Proteus vulgaris ATCC 8427, Pseudomonas aeruginosa ATCC 27853, Listeria monocytogenes ATCC 15313, Klebsiella pneumoniae UC57, Bacillus cereus ATCC 7064, Escherichia coli ATCC 10536, Rhodotorula rubra DSM 70403, Candida albicans ATCC 10231 and Kluyveromyces fragilis ATCC 8608 were used as test microorgams. We have found that the ethanol extract of the lichen has a potential antimicrobial effect against all tested microorganisms, forming inhibition zones between 12.6-22.4 mm, as compared with the standard antibiotics. Notably, the extract has a strong effect against Escherichia coli and Staphylococcus aureus, especially Candida albicans. In conclusion, P. acetabulum may assist in the discovery of new antimicrobial agents that can serve as selective agents or in the preparation of new combined therapeutic drugs. However, the effect of this lichen species on more pathogenic organisms should be investigated and further and detailed pharmacological and toxicological studies should be conducted.

References

  • [1]V. Shukla, G.P. Joshi and M.S.M. Rawat, “Lichens as a potential natural source of bioactive compounds: a review,” Phytochem Rev., vol. 9, no. 2, pp. 303-314, 2010.
  • [2]B. Rankovic and M. Kosanic, “Lichen as a potential source of bioactive secondary metabolites. Lichen secondary metabolites,” Springer International Publishing Switzerland, pp. 1-26, 2015.
  • [3]N.H. Khan NH, M.S.A. nur-E Kamal and M. Rahman, “Antibacterial activity of Euphorbia thymifolia Linn,” Indian J Med Res., vol. 87, pp. 395-397, 1988.
  • [4]CLSI. Performance standards for antimicrobial disk susceptibility tests, approved standard-eleventh edition. CLSI document M02-A11, vol. 32, no. 1, Clinical and Laboratory Standards Institute, Wayne, PA, 2012.
  • [5] NCCLS. Method for antifungal disk diffusion susceptibility testing of yeasts: proposed guideline M44-P. National Committee for Clinical Laboratory Standards, Wayne, PA, 2003.
  • [6]C.H. Collins, P.M. Lyne and G.M. Grange, “Collins and Lyne Microbiological methods,” 6th ed., London, UK: Butterworth, 1989.
  • [7]J. Tomovic, M., Kosanic, S. Ristic, B. Rankoviv, T. Stanojkovic and N. Monojlovic, “Chemical composition and bioactive properties of the lichen, Pleurosticta acetabulum,” Trop J Pharm Res., vol. 16, no. 12, pp. 2977-2984, 2017.
  • [8]N. Manojlovic, B. Rankovic, M. Kosanic, P. Vasiljevic and T. Stanojkovic, “Chemical composition of three Parmelia lichens and antioxidant, antimicrobial and cytotoxic activities of some their major metabolites,” Phytomed, vol. 19, no. 13, pp. 1116-1172, 2012.
  • [9]Y. Yilmaz, A.O. Turk, T. Tay and M. Kivanc, “The antimicrobial activity of extracts of the lichen Cladonia foliaceae and its (-)-usnic acid, atronin and fumarprotocetraric acid constituents,” Z Naturforsch C, vol. 59, no. 3-4, pp. 249-254, 2004.
  • [10]V.M. Thadhani, I.M. Choudhary, S. Khan and V. Karunaratne, “Antimicrobial and toxicolojical activities of some depsides and depsidones,” J Natl Sci Found, vol. 40, pp. 43-48, 2012.
  • [11]E. Ucarkus, B. Gokalsin, N. Yildirim, B. Acikgoz, G. Ozyigitoglu and N.C. Sesal, “Antibacterial activities of extracts of lichen samples collected from Balikesir region on Escherichia coli ATCC 25922,” Journal of Turkish Society of Microbiology, vol. 47, pp. 26-32, 2017.

Pleurosticta acetabulum Liken Türünün Antimikrobiyal Aktivitesi Üzerine Değerlendirme

Year 2023, Volume: 11 Issue: 1, 199 - 203, 31.01.2023
https://doi.org/10.29130/dubited.1082155

Abstract

Bu çalışmada, Pleurosticta acetabulum (Neck.) Elix & Lumbsch (Parmeliaceae) liken türünden elde edilen etanol ekstraktının antimikrobiyal aktivitesi, disk difüzyon yöntemi kullanılarak, Mycobacterium smegmatis CCM 2067, Micrococcus luteus CCM 169, Staphylococcus aureus ATCC 6538P, Proteus vulgaris ATCC 8427, Pseudomonas aeruginosa ATCC 27853, Listeria monocytogenes ATCC 15313, Klebsiella pneumoniae UC57, Bacillus cereus ATCC 7064, Escherichia coli ATCC 10536, Rhodotorula rubra DSM 70403, Candida albicans ATCC 10231 ve Kluyveromyces fragilis ATCC 8608 test mikroorganizmalarına karşı araştırılmıştır. Standart antibiyotikleri ile karşılaştırıldığında, elde edilen etanol ekstraktının tüm test mikroorganizmalarına karşı (12.6-22.4 mm değerleri arasında) potansiyel bir aktivite gösterdiği belirlenmiştir. Sonuç olarak, P. acetabulum seçici ajan olarak hizmet edebilecek yeni antimikrobiyal ajanların keşfedilmesinde veya yeni konbine terapotik ilaçların hazırlanmasında yardımcı olabilir. Ancak bu liken türünün daha fazla mikroorganizmalar üzerindeki etkisinin araştırılması, daha ileri ve detaylı farmakolojik ve toksikolojik araştırmaların yapılması gerekmektedir.

References

  • [1]V. Shukla, G.P. Joshi and M.S.M. Rawat, “Lichens as a potential natural source of bioactive compounds: a review,” Phytochem Rev., vol. 9, no. 2, pp. 303-314, 2010.
  • [2]B. Rankovic and M. Kosanic, “Lichen as a potential source of bioactive secondary metabolites. Lichen secondary metabolites,” Springer International Publishing Switzerland, pp. 1-26, 2015.
  • [3]N.H. Khan NH, M.S.A. nur-E Kamal and M. Rahman, “Antibacterial activity of Euphorbia thymifolia Linn,” Indian J Med Res., vol. 87, pp. 395-397, 1988.
  • [4]CLSI. Performance standards for antimicrobial disk susceptibility tests, approved standard-eleventh edition. CLSI document M02-A11, vol. 32, no. 1, Clinical and Laboratory Standards Institute, Wayne, PA, 2012.
  • [5] NCCLS. Method for antifungal disk diffusion susceptibility testing of yeasts: proposed guideline M44-P. National Committee for Clinical Laboratory Standards, Wayne, PA, 2003.
  • [6]C.H. Collins, P.M. Lyne and G.M. Grange, “Collins and Lyne Microbiological methods,” 6th ed., London, UK: Butterworth, 1989.
  • [7]J. Tomovic, M., Kosanic, S. Ristic, B. Rankoviv, T. Stanojkovic and N. Monojlovic, “Chemical composition and bioactive properties of the lichen, Pleurosticta acetabulum,” Trop J Pharm Res., vol. 16, no. 12, pp. 2977-2984, 2017.
  • [8]N. Manojlovic, B. Rankovic, M. Kosanic, P. Vasiljevic and T. Stanojkovic, “Chemical composition of three Parmelia lichens and antioxidant, antimicrobial and cytotoxic activities of some their major metabolites,” Phytomed, vol. 19, no. 13, pp. 1116-1172, 2012.
  • [9]Y. Yilmaz, A.O. Turk, T. Tay and M. Kivanc, “The antimicrobial activity of extracts of the lichen Cladonia foliaceae and its (-)-usnic acid, atronin and fumarprotocetraric acid constituents,” Z Naturforsch C, vol. 59, no. 3-4, pp. 249-254, 2004.
  • [10]V.M. Thadhani, I.M. Choudhary, S. Khan and V. Karunaratne, “Antimicrobial and toxicolojical activities of some depsides and depsidones,” J Natl Sci Found, vol. 40, pp. 43-48, 2012.
  • [11]E. Ucarkus, B. Gokalsin, N. Yildirim, B. Acikgoz, G. Ozyigitoglu and N.C. Sesal, “Antibacterial activities of extracts of lichen samples collected from Balikesir region on Escherichia coli ATCC 25922,” Journal of Turkish Society of Microbiology, vol. 47, pp. 26-32, 2017.
There are 11 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Articles
Authors

Başaran Dülger 0000-0002-3184-2652

Gorkem Dulger 0000-0002-1506-1549

Bahar Gedik

Seyhan Oran 0000-0001-8196-2065

Publication Date January 31, 2023
Published in Issue Year 2023 Volume: 11 Issue: 1

Cite

APA Dülger, B., Dulger, G., Gedik, B., Oran, S. (2023). Evaluation on Antimicrobial Activity of The Lichen Pleurosticta acetabulum. Düzce Üniversitesi Bilim Ve Teknoloji Dergisi, 11(1), 199-203. https://doi.org/10.29130/dubited.1082155
AMA Dülger B, Dulger G, Gedik B, Oran S. Evaluation on Antimicrobial Activity of The Lichen Pleurosticta acetabulum. DUBİTED. January 2023;11(1):199-203. doi:10.29130/dubited.1082155
Chicago Dülger, Başaran, Gorkem Dulger, Bahar Gedik, and Seyhan Oran. “Evaluation on Antimicrobial Activity of The Lichen Pleurosticta Acetabulum”. Düzce Üniversitesi Bilim Ve Teknoloji Dergisi 11, no. 1 (January 2023): 199-203. https://doi.org/10.29130/dubited.1082155.
EndNote Dülger B, Dulger G, Gedik B, Oran S (January 1, 2023) Evaluation on Antimicrobial Activity of The Lichen Pleurosticta acetabulum. Düzce Üniversitesi Bilim ve Teknoloji Dergisi 11 1 199–203.
IEEE B. Dülger, G. Dulger, B. Gedik, and S. Oran, “Evaluation on Antimicrobial Activity of The Lichen Pleurosticta acetabulum”, DUBİTED, vol. 11, no. 1, pp. 199–203, 2023, doi: 10.29130/dubited.1082155.
ISNAD Dülger, Başaran et al. “Evaluation on Antimicrobial Activity of The Lichen Pleurosticta Acetabulum”. Düzce Üniversitesi Bilim ve Teknoloji Dergisi 11/1 (January 2023), 199-203. https://doi.org/10.29130/dubited.1082155.
JAMA Dülger B, Dulger G, Gedik B, Oran S. Evaluation on Antimicrobial Activity of The Lichen Pleurosticta acetabulum. DUBİTED. 2023;11:199–203.
MLA Dülger, Başaran et al. “Evaluation on Antimicrobial Activity of The Lichen Pleurosticta Acetabulum”. Düzce Üniversitesi Bilim Ve Teknoloji Dergisi, vol. 11, no. 1, 2023, pp. 199-03, doi:10.29130/dubited.1082155.
Vancouver Dülger B, Dulger G, Gedik B, Oran S. Evaluation on Antimicrobial Activity of The Lichen Pleurosticta acetabulum. DUBİTED. 2023;11(1):199-203.