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Sonofotodinamik terapi - Kutanöz Leishmaniasis tedavisinde yeni bir yöntem: Bir in vitro çalışma

Year 2022, Volume: 5 Issue: 3, 84 - 91, 30.12.2022
https://doi.org/10.33713/egetbd.1199582

Abstract

AMAÇ: Bu çalışmada, kurkumin aracılı sonodinamik (SDT), fotodinamik (FDT) ve sonofotodinamik (SFDT) tedavilerin Leishmania tropica (L.tropica) promastigotlarına karşı olası bir mekanizma ile antileishmanial etkinliğini araştırmayı amaçladık. SFDT, sonodinamik ve fotodinamik tedavileri birleştiren Leishmaniasis tedavisine yeni bir yaklaşımdır. Curcumin, uzun yıllardır tıbbi durumlarda da kullanılan doğal bir anti-inflamatuar ajandır. Curcumin, bu çalışmada PDT, SDT, SPDT'nin L.tropica promastigotları üzerindeki etkisini karşılaştırmak için hem sonosensitizer hem de fotosensitizör olarak kullanılmıştır.
GEREÇ VE YÖNTEM: Hücreler, farklı konsantrasyonlarda (0.25, 1,4,16 ve 64μM) curcumin ile bir saat süreyle inkübe edildi, 1 MHz frekansında 3 W/cm2 yoğunluklu ultrasona ve/veya 30 dakika süreyle 1.32 J/cm2 ışık ışımasına maruz bırakıldı. Aynı zamanda parazit hücreleri sadece ultrason ve ışık ile ve her ikisi de kurkumin varlığında veya yokluğunda SFDT için maruz bırakıldı. Hücre canlılığını değerlendirmek için XTT ve morfolojik değişiklikleri belirlemek için giemsa boyaması kullanıldı.
BULGULAR: Kurkumin ve ultrason, kurkumin ve ışık, kurkumin aracılı ultrason ve ışık kombinasyonu ile L.tropica promastigot canlılığının kontrol, ultrason-kontrol ve ışık kontrol grubuna göre azaldığı bulundu. En büyük azalmanın SPDT grubunda olduğu tespit edildi. Giemsa boyama bulguları, kurkumin aracılı SDT, PDT ve SPDT'nin L.tropica promastigotlarında atipik çeşitli morfolojik değişikliklere neden olduğunu göstermiştir. Bu sonuçlar ile SPDT'nin L.tropica promastigotları üzerinde diğer tedavilerden daha etkili olduğu bulunmuştur.
SONUÇ: Curcumin aracılı SPDT, L.tropica promastigotları için umut verici bir yaklaşım sağlayabilir.

Supporting Institution

Türkiye Bilimsel ve Teknolojik Araştırma Kurumu-TÜBİTAK 2209A Üniversite Öğrencileri Araştırma Projeleri Destekleme Programı

Project Number

1919B012112125

Thanks

Bu çalışma, Türkiye Bilimsel ve Teknolojik Araştırma Kurumu-TÜBİTAK, Proje no: 1919B012112125 tarafından mali olarak desteklenmiştir. L.tropica promastigotlarını bize sağladığı için Prof.Dr.Hatice ERTABAKLAR'a teşekkür ederiz.

References

  • 1.WHO. Leishmaniasis in high-burden countries: an epidemiological update based on data reported in 2014. Wkly Epidemiol Rec. 2016; 91: 287-96.
  • 2. WHO. Control of the leishmaniases. World Health Organ Tech Rep Ser. 2010; 949: xii-xiii, 1-186.
  • 3. Hepburn NC. Cutaneous leishmaniasis. Clin Exp Dermatol.2000; 25: 363- 70.
  • 4. Özbilgin A, Çavuş İ, Yıldırım A, Kaya T, Ertabaklar H. Evaluation of In vitro and In vivo Drug Efficacy Over Leishmania tropica: A Pilot Study. Turkiye Parazitol Derg. 2018; 42: 11-9.
  • 5. Berman J. Current treatment approaches to leishmaniasis. Curr Opin Infect Dis. 2003; 16: 397-401.
  • 6. Kennedy J. High-intensity focused ultrasound in the treatment of solid tumours. Nat Rev Cancer. 2005; 5:321-327.
  • 7. Nikfarjam M, Christophi C. Interstitial laser thermotherapy for liver tumours. Br J Surg. 2003; 90:1033- 1047. 8.Shavkuta BS, Gerasimov MY, Minaev NV,Kuznetsova DS, Dudenkova VV, Mushkova IA, Malyugin BE, Kotova SL, Timashev PS, Kostenev SV. Highly effective 525 nm femtosecond laser crosslinking of collagen and strengthening of a human donor cornea. Laser Phys Lett. 2018; 15:015602.
  • 9.Saito M, Lida T, Nagayama D. Photodynamic therapy with verteporfin for age-related macular degeneration or polypoidal choroidal vasculopathy: comparison of the presence of serous retinal pigment epithelial detachment. Br J Ophthalmol.2008; 92: 1642-1647.
  • 10. Dolmans D, Fukumura D, Jain R. Photodynamic therapy for cancer Nat Rev Cancer,.2003; 3:380-387.
  • 11. Nesi-Reis V, Lera-Nonose D, Oyama J, Silva-Lalucci MPP, Demarchi IG, Aristides SMA, Teixeira JJV, Silveira TGV, Lonardoni MVC. Contribution of Photodynamic Therapy in Wound Healing: A Systematic Review. Photodiagnosis Photodyn Ther. 2017; 21:294-305.
  • 12. Wen X, Li Y, Hamblin MR. Photodynamic therapy in dermatology beyond non-melanoma cancer: An update. Photodiagnosis Photodyn Ther. 2017; 19:140-152.
  • 13. Bashkatov AN, Genina EA, Kochubey VI, Tuchin VV. Optical properties of human skin, subcutaneous and mucous tissues in the wavelength range from 400 to 2000 nm. J Phys D Appl Phys. 2005; 38:2543- 2555.
  • 14. Ju D, Yamaguchi F, Zhan G, Higuchi T, Asakura T, Morita A, Orimo H, Hu S. Hyperthermotherapy enhances antitumor effect of 5-aminolevulinic acid-mediated sonodynamic therapy with activation of caspase-dependent apoptotic pathway in human glioma. Tumor Biol. 2016;37:10415-10426.
  • 15.Liu F, Wu L, Chen L, Qi X, Ge Y, Shen S. Ultrasound-guided tumor sonodynamic therapy based on sonosensitizer liposome. Chem Lett. 2016;45:1304-1306.
  • 16.Wan GY, Liu Y, Chen BW, Liu YY, Wang YS, Zhang N. Recent advances of sonodynamic therapy in cancer treatment. Cancer Biol Med. 2016;13:325-338.
  • 17. Jin ZH, Miyoshi N, Ishiguro K, Umemura S, Kawabata K, Yumita N, Sakata I, Takaoka K, Udagawa T, Nakajima S, Tajiri H, Ueda K, Fukuda M, Kumakiri M. Combination Effect of Photodynamic and Sonodynamic Therapy on Experimental Skin Squamous Cell Carcinoma in C3H/HeN Mice. J Dermatol. 2000; 27:294-306.
  • 18. Tserkovsky DA, Alexandrova EN, Chalau, VN, Istomin YP .Effects of Combined Sonodynamic and Photodynamic Therapies with Photolon on a Glioma C6 Tumor Model. Exp Oncol. 2012; 34:332-335.
  • 19. Zhou H, Beevers CS and Huang S. The targets of curcumin. Curr Drug Targets. 2011; 12: 332-347.
  • 20. Nose M, Koide T, Ogihara Y, Yabu Y, Ohta N, Trypanocidal Effects of Curcumin in Vitro Biol. Pharm. Bull. 1998;21: 643-645.
  • 21. Rasmussen HB, Christensen SB, Kvist LP, Karazmi A. A simple and efficient separation of the curcumins, the antiprotozoal constituents of Curcuma longa. Planta Med. 2000; 66:396-398.
  • 22. Gomes C, Alegrio LV, De Lima M, Leon LL, Araujo C. Synthetic derivatives of curcumin and their activity against Leishmania amazonensis. Arzneimittelforschung. 2001; 52:120-124.
  • 23. Changtam C, de Koning HP, Ibrahim H, Sajid MS, Gould MK, Suksamrarn A. Curcuminoid analogs with potent activity against Trypanosoma and Leishmania species. Eur J Med Chem. 2010;45:941-956.
  • 24. Ayala ETP, De Sousa FAD, Filho JDV, Garcia MR, De Boni L, Bagnato VS, Pratavieira. Photodynamic and Sonodynamic Therapy with Protoporphyrin IX: In Vitro and In Vivo Studies. Ultrasound Med Biol. 2021;47:1032-1044
  • 25. Sadanala KC, Chaturvedi PK, Seo YM, Kim JM, Jo YS, Lee YK, Ahn WS. Sono-photodynamic combination therapy: a review on sensitizers. Anticancer Res. 2014;34:4657-4664.
  • 26.Ozlem-Caliskan S, Ozen H, Kaya I, Ilıkcı-Sagkan R, Ertabaklar H. An In Vitro Study on Sonodynamic Therapy of Leishmania tropica Using Curcumin. Mikrobiyol Bul. 2022;56:706-721.
  • 27.Pereira AHC, Marcolino LMC, Pinto JG, Ferreira-Strixino J. Evaluation of the photodynamic therapy with curcumin on L.braziliensis and L.major amastigotes. Antibiotics. 2021; 10: 634.
  • 28.De Melo PBG, Besegato JF, Bernardi ACA, Bagnato VS , Rastelli ANS. Can sono-photodynamic therapy enhance the antibacterial effect of curcumin against Streptococcus mutans biofilm? Laser Phys Lett. 2021;18:105601
  • 29. Zongfang W, Chengcheng L, Yiming Z, Min H, Dandan M, Zhang P, Xue Y, Li X. Photomagnetic nanoparticles in dual-modality imaging and photo-sonodynamic activity against bacteria. J Chem Eng. 2019; 356:811-818.
  • 30. Bhavya ML, Hebbar HU. Sono-photodynamic inactivation of Escherichia coli and Staphylococcus aureus in orange juice. Ultrason Sonochem. 2019;57:108-115.
  • 31. Zhang J , Zheng P , Li J, Yang Y, Zeng S, Qiu J, Lin S. Curcumin-Mediated Sono-Photodynamic Treatment Inactivates Listeria monocytogenes via ROS-Induced Physical Disruption and Oxidative Damage. Foods. 2022, 11:808
  • 32. Niavarzi S, Pourhajibagher M, Khedmat S, Ghabraei S, Chiniforush N, Ba-hador A. Effect of ultrasonic activation on the efficacy of antimicrobial photody- namic therapy: evaluation of penetration depth of photosensitizer and elimination of Enterococcus faecalis biofilms. Photodiagn Photodyn Ther. 2019; 27:362-366.
  • 33. Pourhajibagher M, Rokn RA, Barikani HR, Bahador A. Photo-sonodynamic antimicrobial chemotherapy via chitosan nanoparticles-indocyanine green against polymicrobial periopathogenic biofilms: ex vivo study on dental implants, Photodiagn Photodyn Ther. 2020; 31:101834.
  • 34. Trendowski M. The promise of sonodynamic therapy .Cancer Metastasis Rev. 2014;33:143-160.
  • 35. Abd El-Kaream SA, Abd Elsamie GH, Abd-Alkareem AS. Sono-photodynamic modality for cancer treatment using bio-degradable bio-conjugated sonnelux nanocomposite in tumor-bearing mice: activated cancer therapy using light and ultrasound. Biochem Biophys Res Commun. 2018;503: 1075-1086.
  • 36. Chen HJ, Zhou XB ,Wang AL, Zheng BY, Yeh CK, Huang JD. Synthesis and biological characterization of novel rose bengal derivatives with improved amphiphilicity for sono-photodynamic therapy Eur J Med Chem. 2018; 145:86-95.

Sonophotodynamic therapy-a new method in the treatment of Cutaneous Leishmaniasis: An in vitro study

Year 2022, Volume: 5 Issue: 3, 84 - 91, 30.12.2022
https://doi.org/10.33713/egetbd.1199582

Abstract

OBJECTİVE: In this study, we aimed to examine of antileishmanial effect of curcumin-mediated sonodynamic (SDT), photodynamic (PDT), and sonophotodynamic (SPDT) therapies with a potential mechanism against the Leishmania tropica (L.tropica) promastigotes. SPDT is a new treatment modality for Leishmaniasis that combines photodynamic and sonodynamic therapies. Curcumin is a natural antiinflammatory agent that has been used for treating medical conditions for many years. Curcumin was used in this study both as a sonosensitizer and photosensitizer to compare the effect of PDT, SDT, SPDT on L.tropica promastigotes.
MATERIALS AND METHODS: The cells were incubated with different concentrations (0.25, 1,4,16 and 64μM) of curcumin for 1 hour, were exposed to 3 W/cm2 intensity ultrasound for 1MHz frequency and/or subjected to 1,32 J/cm2 light irradiation for 30 minutes. Also, parasite cells were exposed for SPDT with ultrasound and light only and both in the presence or absence of curcumin. XTT was used to evaluate cell viability and giemsa staining was used to determine morphological changes.
RESULTS: With the combination of curcumin and ultrasound, curcumin and light, curcumin mediated ultrasound and light,L.tropica promastigote viability was found to be decreased compared to the control, ultrasound-control and light-control group. The greatest reduction was found in the SPDT group. Giemsa staining findings showed that curcumin-mediated SDT, PDT and SPDT induced several morphological alterations in L.tropica promastigotes atypical. These results showed that SPDT is more effective than other therapies on L.tropica promastigotes
CONCLUSIONS: Curcumin-mediated SPDT may provide a promising approach for L.tropica promastigotes.

Project Number

1919B012112125

References

  • 1.WHO. Leishmaniasis in high-burden countries: an epidemiological update based on data reported in 2014. Wkly Epidemiol Rec. 2016; 91: 287-96.
  • 2. WHO. Control of the leishmaniases. World Health Organ Tech Rep Ser. 2010; 949: xii-xiii, 1-186.
  • 3. Hepburn NC. Cutaneous leishmaniasis. Clin Exp Dermatol.2000; 25: 363- 70.
  • 4. Özbilgin A, Çavuş İ, Yıldırım A, Kaya T, Ertabaklar H. Evaluation of In vitro and In vivo Drug Efficacy Over Leishmania tropica: A Pilot Study. Turkiye Parazitol Derg. 2018; 42: 11-9.
  • 5. Berman J. Current treatment approaches to leishmaniasis. Curr Opin Infect Dis. 2003; 16: 397-401.
  • 6. Kennedy J. High-intensity focused ultrasound in the treatment of solid tumours. Nat Rev Cancer. 2005; 5:321-327.
  • 7. Nikfarjam M, Christophi C. Interstitial laser thermotherapy for liver tumours. Br J Surg. 2003; 90:1033- 1047. 8.Shavkuta BS, Gerasimov MY, Minaev NV,Kuznetsova DS, Dudenkova VV, Mushkova IA, Malyugin BE, Kotova SL, Timashev PS, Kostenev SV. Highly effective 525 nm femtosecond laser crosslinking of collagen and strengthening of a human donor cornea. Laser Phys Lett. 2018; 15:015602.
  • 9.Saito M, Lida T, Nagayama D. Photodynamic therapy with verteporfin for age-related macular degeneration or polypoidal choroidal vasculopathy: comparison of the presence of serous retinal pigment epithelial detachment. Br J Ophthalmol.2008; 92: 1642-1647.
  • 10. Dolmans D, Fukumura D, Jain R. Photodynamic therapy for cancer Nat Rev Cancer,.2003; 3:380-387.
  • 11. Nesi-Reis V, Lera-Nonose D, Oyama J, Silva-Lalucci MPP, Demarchi IG, Aristides SMA, Teixeira JJV, Silveira TGV, Lonardoni MVC. Contribution of Photodynamic Therapy in Wound Healing: A Systematic Review. Photodiagnosis Photodyn Ther. 2017; 21:294-305.
  • 12. Wen X, Li Y, Hamblin MR. Photodynamic therapy in dermatology beyond non-melanoma cancer: An update. Photodiagnosis Photodyn Ther. 2017; 19:140-152.
  • 13. Bashkatov AN, Genina EA, Kochubey VI, Tuchin VV. Optical properties of human skin, subcutaneous and mucous tissues in the wavelength range from 400 to 2000 nm. J Phys D Appl Phys. 2005; 38:2543- 2555.
  • 14. Ju D, Yamaguchi F, Zhan G, Higuchi T, Asakura T, Morita A, Orimo H, Hu S. Hyperthermotherapy enhances antitumor effect of 5-aminolevulinic acid-mediated sonodynamic therapy with activation of caspase-dependent apoptotic pathway in human glioma. Tumor Biol. 2016;37:10415-10426.
  • 15.Liu F, Wu L, Chen L, Qi X, Ge Y, Shen S. Ultrasound-guided tumor sonodynamic therapy based on sonosensitizer liposome. Chem Lett. 2016;45:1304-1306.
  • 16.Wan GY, Liu Y, Chen BW, Liu YY, Wang YS, Zhang N. Recent advances of sonodynamic therapy in cancer treatment. Cancer Biol Med. 2016;13:325-338.
  • 17. Jin ZH, Miyoshi N, Ishiguro K, Umemura S, Kawabata K, Yumita N, Sakata I, Takaoka K, Udagawa T, Nakajima S, Tajiri H, Ueda K, Fukuda M, Kumakiri M. Combination Effect of Photodynamic and Sonodynamic Therapy on Experimental Skin Squamous Cell Carcinoma in C3H/HeN Mice. J Dermatol. 2000; 27:294-306.
  • 18. Tserkovsky DA, Alexandrova EN, Chalau, VN, Istomin YP .Effects of Combined Sonodynamic and Photodynamic Therapies with Photolon on a Glioma C6 Tumor Model. Exp Oncol. 2012; 34:332-335.
  • 19. Zhou H, Beevers CS and Huang S. The targets of curcumin. Curr Drug Targets. 2011; 12: 332-347.
  • 20. Nose M, Koide T, Ogihara Y, Yabu Y, Ohta N, Trypanocidal Effects of Curcumin in Vitro Biol. Pharm. Bull. 1998;21: 643-645.
  • 21. Rasmussen HB, Christensen SB, Kvist LP, Karazmi A. A simple and efficient separation of the curcumins, the antiprotozoal constituents of Curcuma longa. Planta Med. 2000; 66:396-398.
  • 22. Gomes C, Alegrio LV, De Lima M, Leon LL, Araujo C. Synthetic derivatives of curcumin and their activity against Leishmania amazonensis. Arzneimittelforschung. 2001; 52:120-124.
  • 23. Changtam C, de Koning HP, Ibrahim H, Sajid MS, Gould MK, Suksamrarn A. Curcuminoid analogs with potent activity against Trypanosoma and Leishmania species. Eur J Med Chem. 2010;45:941-956.
  • 24. Ayala ETP, De Sousa FAD, Filho JDV, Garcia MR, De Boni L, Bagnato VS, Pratavieira. Photodynamic and Sonodynamic Therapy with Protoporphyrin IX: In Vitro and In Vivo Studies. Ultrasound Med Biol. 2021;47:1032-1044
  • 25. Sadanala KC, Chaturvedi PK, Seo YM, Kim JM, Jo YS, Lee YK, Ahn WS. Sono-photodynamic combination therapy: a review on sensitizers. Anticancer Res. 2014;34:4657-4664.
  • 26.Ozlem-Caliskan S, Ozen H, Kaya I, Ilıkcı-Sagkan R, Ertabaklar H. An In Vitro Study on Sonodynamic Therapy of Leishmania tropica Using Curcumin. Mikrobiyol Bul. 2022;56:706-721.
  • 27.Pereira AHC, Marcolino LMC, Pinto JG, Ferreira-Strixino J. Evaluation of the photodynamic therapy with curcumin on L.braziliensis and L.major amastigotes. Antibiotics. 2021; 10: 634.
  • 28.De Melo PBG, Besegato JF, Bernardi ACA, Bagnato VS , Rastelli ANS. Can sono-photodynamic therapy enhance the antibacterial effect of curcumin against Streptococcus mutans biofilm? Laser Phys Lett. 2021;18:105601
  • 29. Zongfang W, Chengcheng L, Yiming Z, Min H, Dandan M, Zhang P, Xue Y, Li X. Photomagnetic nanoparticles in dual-modality imaging and photo-sonodynamic activity against bacteria. J Chem Eng. 2019; 356:811-818.
  • 30. Bhavya ML, Hebbar HU. Sono-photodynamic inactivation of Escherichia coli and Staphylococcus aureus in orange juice. Ultrason Sonochem. 2019;57:108-115.
  • 31. Zhang J , Zheng P , Li J, Yang Y, Zeng S, Qiu J, Lin S. Curcumin-Mediated Sono-Photodynamic Treatment Inactivates Listeria monocytogenes via ROS-Induced Physical Disruption and Oxidative Damage. Foods. 2022, 11:808
  • 32. Niavarzi S, Pourhajibagher M, Khedmat S, Ghabraei S, Chiniforush N, Ba-hador A. Effect of ultrasonic activation on the efficacy of antimicrobial photody- namic therapy: evaluation of penetration depth of photosensitizer and elimination of Enterococcus faecalis biofilms. Photodiagn Photodyn Ther. 2019; 27:362-366.
  • 33. Pourhajibagher M, Rokn RA, Barikani HR, Bahador A. Photo-sonodynamic antimicrobial chemotherapy via chitosan nanoparticles-indocyanine green against polymicrobial periopathogenic biofilms: ex vivo study on dental implants, Photodiagn Photodyn Ther. 2020; 31:101834.
  • 34. Trendowski M. The promise of sonodynamic therapy .Cancer Metastasis Rev. 2014;33:143-160.
  • 35. Abd El-Kaream SA, Abd Elsamie GH, Abd-Alkareem AS. Sono-photodynamic modality for cancer treatment using bio-degradable bio-conjugated sonnelux nanocomposite in tumor-bearing mice: activated cancer therapy using light and ultrasound. Biochem Biophys Res Commun. 2018;503: 1075-1086.
  • 36. Chen HJ, Zhou XB ,Wang AL, Zheng BY, Yeh CK, Huang JD. Synthesis and biological characterization of novel rose bengal derivatives with improved amphiphilicity for sono-photodynamic therapy Eur J Med Chem. 2018; 145:86-95.
There are 35 citations in total.

Details

Primary Language English
Subjects Clinical Sciences
Journal Section Original Investigation
Authors

Serçin Özlem Çalışkan 0000-0001-8464-5487

Hayriye Tanem Yavaşal 0000-0001-6038-2758

Project Number 1919B012112125
Early Pub Date December 30, 2022
Publication Date December 30, 2022
Acceptance Date November 21, 2022
Published in Issue Year 2022 Volume: 5 Issue: 3

Cite

EndNote Özlem Çalışkan S, Yavaşal HT (December 1, 2022) Sonophotodynamic therapy-a new method in the treatment of Cutaneous Leishmaniasis: An in vitro study. Ege Tıp Bilimleri Dergisi 5 3 84–91.

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