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D Vitamini Reseptörü Rs1544410 Polimorfizminin Diş Çürüğü Oluşumuna Etkisi

Year 2018, Volume: 2 Issue: 1, 1 - 5, 29.06.2018

Abstract

D vitamininin kas ve kemik gelişimindeki etkilerinden dolayı
ağız ve diş sağlığı açısından önemli etkileri bulunmaktadır. Ağız
hijyenini belirten en önemli ölçeklerden biri DMF-T (Çürük, kayıp,
dolgulu dişler) indeksidir. Çalışmamızda D vitamini reseptörü
(VDR) üzeirnde bulunan rs1544410 polimorfizmi ile oral hijyen
indeksi olan DMF-T arasındaki ilişkisi araştırılmıştır. Çalışmamızda
11’i kız, 13’ü erkek, toplamda 24 sporcuda katılmıştır. Çalışmamıza
gönüllü olarak katılan atletlerin DNA eldesi periferik kandan
ticari kit kullanılarak elde edilmiştir. Sporcuların genotiplerinin
belirlenmesi için gerçek zamanlı polimeraz zincir reaksiyonu (GZPZR
) metodu kullanılmıştır. Bireylerin diş çürük analizlerinin
değerlendirilmesinde de DMF-T indeksinden yararlanılmıştır.
rs1544410 polimorfizmi için elde edilen bulgulara göre çalışmaya
katılan atletlerin AA, AG, GG genotipleri sırasıyla 8 (%33),
13 (%54), 3(%12) GG olarak bulunmuştur. Allelik dağılımlar
incelendiğinde A alleli çalışma grubunda %60, G alleli %40
oranında bulunmuştur. rs1544410 polimorfizminde DMFT analizi
ile genotipler karşılaştırıldığında AA genotipli bireylerde 0-1 arası 2
sporcu (%25) , 2-4 arası 4 sporcu (%50), 5-10 arası 2 sporcu (%25)
bulunurken, 10 ve üzeri değerde hiçbir sporcuya rastlanılmamıştır.
AG genotipli bireylerde ise 0-1, 2-4, 5-10, 10 ve üzeri değerlere
sahip birey sayıları ve yüzdeleri; sırasıyla; 3 (%23), 3(%23), 5
(%38), 2 (%16) şeklinde bulunmuştur. GG genotipli bireylerde
DMFT indeksi 0-1 arası değere sahip olan 2 sporcu (%67), 2-4 arası
değere sahip olan 1 sporcuda (%33) tespit edilmiş olup, 5-10 ile 10
ve üzeri değere hiçbir bireylerde rastlanılmamıştır. Bu çalışmanın,
VDR geninin rs1544410 polimorfizmlerinin diş çürüğü oluşumuna
etkisinin araştırılacağı diğer çalışmalara veri kaynağı olabileceğini
düşünmekteyiz.

References

  • Bachrach FH and Young M. A comparison of the degree of resemblance in dental characters shown in pairs of twins of identical and fraternal types. Br Dent J. 1927;48:1293 – 304
  • Ulucan K, Pul U, Akçay, T. Diş çürüklerinin oluşumuna moleküler yaklaşım. Journal of Cell and Molecular Biology. 2010; 8(2):35-39.
  • Holick MF. Vitamin D deficiency. N Engl J Med. 2007; 357: 266-281
  • Taymans SE, Pack S, Pack E, Orban Z, Barsony J, Zhaung Z and Stratakis CA:The human vitamin Dreceptor gene (VDR) is localized to region 12cen –q12by fluorescent in situ hybridization and radiotion hybrid mapping :Genetic and physical VDR map. J Bone Miner Res.1999;14:1163-1166.
  • Norman AW. Vitamin D receptor: new assignments for an already busy receptor. Endocrinology .2006;147: 5542-5548.
  • Gunes S, Sumer P, Keles C, Kara N, Koprulu H, Bagci H, and Bek Y. Analysis of vitamin D receptor gene polymorphisms in patients with chronic periodontitis. Indian J Med Res. 2008;127:58-64.
  • Ulucan K, Akyüz S, Özbay G, Pekiner FN, Güney AI. Evaluation of Vitamin D Receptor (VDR) Gene Polymorphisms (FokI, TaqI and ApaI) in a Family with Dentinogenesis Imperfecta. Cytology and Genetics. 2013; 47(5): 282-286.
  • Qin X, Lu, Yu M Med, Qin, Aiping.et all. Vitamin D Receptor BsmІ Polymorphism and Ovarian Cancer Risk A Meta-Analysis.International Journal of Gynecological Cancer.2013; 23(7): 1178-1183.
  • Uitterlinden A.G., Fang Y., Bergink A.P, van Meurs J., Leeuwen H.,&Pols H. The role vitamin D receptor gene polymorphism in bone biyology.Moleculer and Cellular Endocrinology.2002;197:15-21
  • Denzer N, Vogt T, Reichrath J. Vitamin D receptor (VDR) polymorphisms and skin cancer: A systematic review. Dermato-Endocrinology. 2011; 3(3): 205-210.
  • Sengün A, Duran I, Erdal ME, Ozkaya M, Ozturk B and Ozer F. Vitamin D Receptır Gene Polymorhism is associated with Dental caries., 81st Gen.Sess.Of Int. Assoc. For Dental Resarch.2003.
  • Wang C, Zhao H, Xiao L, Xie, C, Fan W, Sun S, Xie B, Zhang J. Association Between Vitamin D Receptor Gene Polymorphisms and Severe Chronic Periodontitis in a Chinese Population. Journal of Periodontology. 2009;80(4):603-608.
  • Kong, Y.Y., Zheng, J.M., Zhang,J.W., Jiang, Q.Z., Yang,O., Yu,M., Z.S. The relationship between vitamin D receptor gene polymorphism and deciduous tooth decay in Chinese children. BMC Oral Health 2017; 17:111.
  • Li S, Yang MH, Zeng CA, Wu WL, Huang XF, Ji Y, Zeng JQ. Association of vitamin D receptor gene polymorphisms in Chinese patients with generalized aggressive periodontitis. J Periodontal Res. 2008;43(3):360-363.

Effect of Vitamin D Receptor rs1544410 Polymorphism on Tooth Decays

Year 2018, Volume: 2 Issue: 1, 1 - 5, 29.06.2018

Abstract

Due to the effects of vitamin D on muscle and bone development,
there are significant effects on oral and dental health. One of the
important markers of oral health is the index (DMF-T), which
reports the number of caries, missing filled teeth. In our study,
the relationship between rs1544410 polymorphism on D vitamine
receptor (VDR) and the number of teeth affected by caries and
results was investigated. A total of 24 athletes, 11 girls and 13
boys, participated in the study. The DNA samples of the athletes
were isolated from buccal cells by using a commercially avaible
DNA isolation Kit. The real-time polymerase chain reaction (RTPCR)
method was used to determine the genotypes of the athletes.
The DMF-T index was used to identify individuals with caries,
missing filled teeth. Respective number and percentages of AA,
AG, GG genotypes were found as 8 (33%), 13 (54%) and 3 (12%)
GG, for rs1544410 polymorphism. When allele distributions were
examined, A allelle was found to be as 60%, and G allelle as 40%.
When genotypes were compared with DMF-T, 2 athletes were
(25%) between 0-1, 4 athletes (50%) between 2-4, 2 athletes (25%)
were between 5-10 , and none of the athletes had above 10 for the
individuals with AA genotypes. For AG genotypes, the respective
numbers and percentages of individuals with values 0-1, 2-4, 5-10,
10 and above are 3 (23%), 3 (23%), 5 (38%) and 2 (16%). For GG
genotypes, the DMFT index was found in 2 athletes with a score of
0-1 (67%) and in 1 (33%) with a score of 2-4. None of the athletes
with GG gonotypes had 5-10 or 10 and above score for DMF-T.
We suggest that this study may be an informative data for further
studies to investigate the effect of rs1544410 polymorphisms on
the formation of tooth decays.

References

  • Bachrach FH and Young M. A comparison of the degree of resemblance in dental characters shown in pairs of twins of identical and fraternal types. Br Dent J. 1927;48:1293 – 304
  • Ulucan K, Pul U, Akçay, T. Diş çürüklerinin oluşumuna moleküler yaklaşım. Journal of Cell and Molecular Biology. 2010; 8(2):35-39.
  • Holick MF. Vitamin D deficiency. N Engl J Med. 2007; 357: 266-281
  • Taymans SE, Pack S, Pack E, Orban Z, Barsony J, Zhaung Z and Stratakis CA:The human vitamin Dreceptor gene (VDR) is localized to region 12cen –q12by fluorescent in situ hybridization and radiotion hybrid mapping :Genetic and physical VDR map. J Bone Miner Res.1999;14:1163-1166.
  • Norman AW. Vitamin D receptor: new assignments for an already busy receptor. Endocrinology .2006;147: 5542-5548.
  • Gunes S, Sumer P, Keles C, Kara N, Koprulu H, Bagci H, and Bek Y. Analysis of vitamin D receptor gene polymorphisms in patients with chronic periodontitis. Indian J Med Res. 2008;127:58-64.
  • Ulucan K, Akyüz S, Özbay G, Pekiner FN, Güney AI. Evaluation of Vitamin D Receptor (VDR) Gene Polymorphisms (FokI, TaqI and ApaI) in a Family with Dentinogenesis Imperfecta. Cytology and Genetics. 2013; 47(5): 282-286.
  • Qin X, Lu, Yu M Med, Qin, Aiping.et all. Vitamin D Receptor BsmІ Polymorphism and Ovarian Cancer Risk A Meta-Analysis.International Journal of Gynecological Cancer.2013; 23(7): 1178-1183.
  • Uitterlinden A.G., Fang Y., Bergink A.P, van Meurs J., Leeuwen H.,&Pols H. The role vitamin D receptor gene polymorphism in bone biyology.Moleculer and Cellular Endocrinology.2002;197:15-21
  • Denzer N, Vogt T, Reichrath J. Vitamin D receptor (VDR) polymorphisms and skin cancer: A systematic review. Dermato-Endocrinology. 2011; 3(3): 205-210.
  • Sengün A, Duran I, Erdal ME, Ozkaya M, Ozturk B and Ozer F. Vitamin D Receptır Gene Polymorhism is associated with Dental caries., 81st Gen.Sess.Of Int. Assoc. For Dental Resarch.2003.
  • Wang C, Zhao H, Xiao L, Xie, C, Fan W, Sun S, Xie B, Zhang J. Association Between Vitamin D Receptor Gene Polymorphisms and Severe Chronic Periodontitis in a Chinese Population. Journal of Periodontology. 2009;80(4):603-608.
  • Kong, Y.Y., Zheng, J.M., Zhang,J.W., Jiang, Q.Z., Yang,O., Yu,M., Z.S. The relationship between vitamin D receptor gene polymorphism and deciduous tooth decay in Chinese children. BMC Oral Health 2017; 17:111.
  • Li S, Yang MH, Zeng CA, Wu WL, Huang XF, Ji Y, Zeng JQ. Association of vitamin D receptor gene polymorphisms in Chinese patients with generalized aggressive periodontitis. J Periodontal Res. 2008;43(3):360-363.
There are 14 citations in total.

Details

Primary Language Turkish
Subjects Dentistry
Journal Section Articles
Authors

Başak Funda Eken This is me

Canan Sercan This is me

Hazal Gezmiş This is me

Özlem Moufti Chousen This is me

Deniz Kıraç This is me

Serap Akyüz This is me

Korkut Ulucan

Publication Date June 29, 2018
Published in Issue Year 2018 Volume: 2 Issue: 1

Cite

APA Eken, B. F., Sercan, C., Gezmiş, H., Moufti Chousen, Ö., et al. (2018). D Vitamini Reseptörü Rs1544410 Polimorfizminin Diş Çürüğü Oluşumuna Etkisi. European Journal of Research in Dentistry, 2(1), 1-5.