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Effect of different exposure times on the amount of residual monomer released from adhesive systems

Year 2013, Volume: 30 Issue: 1, 6 - 12, 11.03.2013

Abstract

OBJECTIVE: The aim of this study was to evaluate the amount of residual monomer released from three different adhesive systems polymerized with four different exposure times.

MATERIALS AND METHOD: The adhesives were applied onto dentin surfaces (length of an edge of 7 mm and 0.9 mm thickness) according to the manufacturer instructions, and polymerized using light curing unit (LED) for 10, 20, 40 and 60 seconds, respectively (n=5). After polymerization, each specimen was immediately immersed in lightproof glass bottle containing 75% ethanol and 25% deionized water at 37 oC. During the period of immersion, samples were removed from the bottles at 10 min, 1 h., 24 h., 7 d. and 30 d. by using micro-pipette. Residual monomers (Bis-GMA, UDMA) eluted from adhesives were analyzed with HPLC. The results were analyzed with one-way analysis of variance and Tukey HSD test.

RESULTS: There was residual monomer release at all time periods. It was observed that the increase in the polymerization time did not have an effect on residual monomer release except for Clearfil S3 Bond (Bis-GMA) and Clearfil Photo Bond (UDMA) groups.

CONCLUSION: Longer exposure times than those recommended by the manufacturers may decrease residual monomer release from some of the adhesives.

References

  • Van Meerbeek B, Van Landuyt K, De Munck J, Inoue S, Yoshida Y, Perdigão J, et al. Bonding to enamel and dentin. Summit JB, Robbins JW, Hilton TJ, Schwartz RS, eds. Fundamentals of Operative Dentistry, 3rd edn. Illinois: Quintessence Publishing; 2006. p.183-260.
  • Van Landuyt KL, Snauwaert J, De Munck J, Peumans M, Yoshida Y, Poitevin A. Systematic review of the chemical composition of contemporary dental adhesives. Biomaterials 2007;28:3757-85.
  • Sideridou ID Achilias DS. Elution Study of Unreacted Bis-GMA, TEGDMA, UDMA, and Bis-EMA from Light-Cured Dental Resins and Resin Composites Using HPLC. J Biomed Mater Res B: Appl Biomater 2005;74:617-26.
  • Rueggeberg FA, Margeson DH. The effect of oxygen inhibition on an unfilled/filled composite system. J Dent Res 1990;69:1652-8.
  • Cardoso Pde C, Loguercio AD, Vieira LC, Baratieri LN, Reis A. Effect of prolonged application times on resin-dentin bond strengths. J Adhes Dent 2005;7:143-9.
  • Nunes TG, Ceballos L, Osorio R, Toledano M. Spatially resolved photopolymerization kinetics and oxygen inhibition in dental adhesives. Biomaterials 2005;26:1809-17.
  • Reis A, Ferreira SQ, Costa TRF, Klein-Junior CA, Meier MM, Loguercio AD. Effects of increased exposure times of simplified etch-and rinse adhesives on the degradation of rezin-dentin bonds and quality of the polymer network. Eur J Oral Sci 2010;118:502-9.
  • Feng L, Carvalho R, Suh BI. Unsufficient cure under the condition of high irridiance and short irridation time. Dent Mater 2009;25:283-9.
  • Moreira F, Filho N, Souza J, Lopez L. Sorption, solubility and residuel monomers of a dental adhesive cured by different light-curing units. Braz Dent J 2010;21:432-8.
  • Hanks CT, Strawn SE, Wataha JC, Craig RG. Cytotoxic effects of resin components on cultured mammalian fibroblasts. J Dent Res 1991;70:1450-5.
  • Noda M, Wataha JC, Kaga M, Lockwooh PE, Volkmann KR, Sano H. Component of dentinal adhesives modulate heat shock protein 72 expression in heatstressed TPH-1 human monocytes at sublethal concentrations. J Dent Res 2002;81:265-9.
  • Ratanasathien S, Wataha JC, Hanks CT, Dennison JB Cytotoxic interactive effects of dentin bondingcomponents on mouse fibroblasts. J Dent Res 1995;74:1602-6.
  • Schwengberg S, Bohlen H, Kleinsasser N, Kehec K, Seiss M, Walther UI, et al. In vitro embryotoxicity assessment with dental restorative materials. J Dent 2005;33:49-55.
  • Ortengren U, Wellendorf H, Harlosson S, Ruyter IE. Water sorption and solubility of dental composites and identification of monomers released in an aqueous environment. J Oral Rehabil 2001;28:1106-15.
  • Moharamzadeh K, Van Noort R, Brook IM, Scut AM. HPLC analysis of components released from dental composites with different resin compositions using different extraction media. J Mater Sci Mater Med 2007;18:133-7.
  • Spahl W, Budzikiewicz H, Geurtsen W. Determination of leachable components from four commercial dental composites by gas and liquid chromatography/mass spectrometry. J Dent 1998;26:137-45.
  • Usümez S, Büyükyilmaz T, Karaman AI, Gündüz B. Degree of conversion of two lingual retainer adhesives cured with different light sources. Eur J Orthod 2005;27:173-9.
  • Ferracane JL. Elution of leachable components from composites. J Oral Rehabil 1994;21:441-52.
  • Ferracane JL, Condon JR. Post cure heat treatments for composites: properties and fractography. Dent Mater 1992;8:290-5.
  • Imazato S, McCabe JF, Tarumi H, Ehara A, Ebisu S. Degree of conversion of composites measured by DTA and FTIR. Dent Mater 2001;17:178-83.
  • Botsalı MS. Rezin esaslı fissur örtücülerin farklı ışık kaynakları ile polimerizasyonu sonrasında açığa çıkan artık monomer miktarının, yüzey sertliğinin ve bağlanma dayanımının araştırılması [tez]. Konya: Selçuk Üniversitesi; 2008.
  • Büyüközer T. Farklı ışık kaynakları ile polimerize edilen çeşitli adeziv sistemlerden farklı dentin kalınlıkları altında salınan artık monomerlerin yüksek basınçlı likit kromatografisi ile analizi [tez]. Konya: Selçuk Üniversitesi; 2009.
  • Schilke R, Lisson JA, Bauss O, Geurtsen W. Comparison of the number and diameter of dentinal tubules in human and bovine dentine by scanning electron microscopic investigation. Arch Oral Biol 2000;45:355-61.
  • Altıntaş S, Usumez A. Evaluation of monomer leaching from a dual cured resin cement. J Biomed Mater Res Part B: Appl Biomater 2008;86:523-9.
  • Söderholm KJ, Mariotti A. BIS-GMA--based resins in dentistry: are they safe? J Am Dent Assoc 1999;130:201-9.
  • Schmalz G. The biocompatiblity of non-amalgam dental filling materials. Eur J Oral Sci 1998;106:696-706.

Farklı polimerizasyon sürelerinin adeziv sistemlerden salınan artık monomer miktarına etkisi

Year 2013, Volume: 30 Issue: 1, 6 - 12, 11.03.2013

Abstract

AMAÇ: Bu çalışmanın amacı, dört farklı polimerizasyon süresinde polimerize edilen üç farklı adeziv sistemin yapılarından salınan artık monomer miktarını tespit etmektir.

GEREÇ VE YÖNTEM: Adeziv sistemler, üretici firmaların talimatlarına uygun olarak kalınlığı 0.9 mm, bir kenar uzunluğu7 mm olan dentin kesitleri üzerine uygulandı. Uygulama sonrası adeziv materyaller 10 sn., 20 sn., 40 sn. ve 60 sn. olmak üzere dört farklı sürede LED ışık cihazı kullanılarak polimerize edildi. Üzerlerine adeziv materyal uygulanarak polimerize edilen dentin kesitleri içlerinde %75’lik etanol/su çözeltisi bulunan amber rengi cam şişelere alındı. Bu çözeltilerden 10. dk., 1. sa., 1. gün, 7. gün ve 30. gün olarak belirlenen zaman periyotlarında mikropipet kullanılarak örnekler alındı. Adezivlerden salınan artık monomer (BisGMA, UDMA) miktarları HPLC cihazı kullanılarak analiz edildi. Grupların genel ve ikili karşılaştırmalarında tek yönlü varyans analizi ve Tukey HSD testi kullanıldı.

BULGULAR: Çalışmada incelenen materyallerden tüm zaman periyotlarında artık monomer salımının gerçekleştiği gözlendi. Polimerizasyon süresinin arttırılmasıyla Clearfil S3 Bond (Bis-GMA) ve Clearfil Photo Bond (UDMA) adeziv materyallerden salınan toplam monomer miktarlarının azaldığı, polimerizasyon süresini arttırmanın diğer materyallerden salınan toplam monomer miktarını etkilemediği gözlendi.

SONUÇ: Üretici firmalar tarafından tavsiye edilen polimerizasyon süresinin arttırılmasıyla bazı adezivlerden salınan monomer miktarı azalabilmektedir.

References

  • Van Meerbeek B, Van Landuyt K, De Munck J, Inoue S, Yoshida Y, Perdigão J, et al. Bonding to enamel and dentin. Summit JB, Robbins JW, Hilton TJ, Schwartz RS, eds. Fundamentals of Operative Dentistry, 3rd edn. Illinois: Quintessence Publishing; 2006. p.183-260.
  • Van Landuyt KL, Snauwaert J, De Munck J, Peumans M, Yoshida Y, Poitevin A. Systematic review of the chemical composition of contemporary dental adhesives. Biomaterials 2007;28:3757-85.
  • Sideridou ID Achilias DS. Elution Study of Unreacted Bis-GMA, TEGDMA, UDMA, and Bis-EMA from Light-Cured Dental Resins and Resin Composites Using HPLC. J Biomed Mater Res B: Appl Biomater 2005;74:617-26.
  • Rueggeberg FA, Margeson DH. The effect of oxygen inhibition on an unfilled/filled composite system. J Dent Res 1990;69:1652-8.
  • Cardoso Pde C, Loguercio AD, Vieira LC, Baratieri LN, Reis A. Effect of prolonged application times on resin-dentin bond strengths. J Adhes Dent 2005;7:143-9.
  • Nunes TG, Ceballos L, Osorio R, Toledano M. Spatially resolved photopolymerization kinetics and oxygen inhibition in dental adhesives. Biomaterials 2005;26:1809-17.
  • Reis A, Ferreira SQ, Costa TRF, Klein-Junior CA, Meier MM, Loguercio AD. Effects of increased exposure times of simplified etch-and rinse adhesives on the degradation of rezin-dentin bonds and quality of the polymer network. Eur J Oral Sci 2010;118:502-9.
  • Feng L, Carvalho R, Suh BI. Unsufficient cure under the condition of high irridiance and short irridation time. Dent Mater 2009;25:283-9.
  • Moreira F, Filho N, Souza J, Lopez L. Sorption, solubility and residuel monomers of a dental adhesive cured by different light-curing units. Braz Dent J 2010;21:432-8.
  • Hanks CT, Strawn SE, Wataha JC, Craig RG. Cytotoxic effects of resin components on cultured mammalian fibroblasts. J Dent Res 1991;70:1450-5.
  • Noda M, Wataha JC, Kaga M, Lockwooh PE, Volkmann KR, Sano H. Component of dentinal adhesives modulate heat shock protein 72 expression in heatstressed TPH-1 human monocytes at sublethal concentrations. J Dent Res 2002;81:265-9.
  • Ratanasathien S, Wataha JC, Hanks CT, Dennison JB Cytotoxic interactive effects of dentin bondingcomponents on mouse fibroblasts. J Dent Res 1995;74:1602-6.
  • Schwengberg S, Bohlen H, Kleinsasser N, Kehec K, Seiss M, Walther UI, et al. In vitro embryotoxicity assessment with dental restorative materials. J Dent 2005;33:49-55.
  • Ortengren U, Wellendorf H, Harlosson S, Ruyter IE. Water sorption and solubility of dental composites and identification of monomers released in an aqueous environment. J Oral Rehabil 2001;28:1106-15.
  • Moharamzadeh K, Van Noort R, Brook IM, Scut AM. HPLC analysis of components released from dental composites with different resin compositions using different extraction media. J Mater Sci Mater Med 2007;18:133-7.
  • Spahl W, Budzikiewicz H, Geurtsen W. Determination of leachable components from four commercial dental composites by gas and liquid chromatography/mass spectrometry. J Dent 1998;26:137-45.
  • Usümez S, Büyükyilmaz T, Karaman AI, Gündüz B. Degree of conversion of two lingual retainer adhesives cured with different light sources. Eur J Orthod 2005;27:173-9.
  • Ferracane JL. Elution of leachable components from composites. J Oral Rehabil 1994;21:441-52.
  • Ferracane JL, Condon JR. Post cure heat treatments for composites: properties and fractography. Dent Mater 1992;8:290-5.
  • Imazato S, McCabe JF, Tarumi H, Ehara A, Ebisu S. Degree of conversion of composites measured by DTA and FTIR. Dent Mater 2001;17:178-83.
  • Botsalı MS. Rezin esaslı fissur örtücülerin farklı ışık kaynakları ile polimerizasyonu sonrasında açığa çıkan artık monomer miktarının, yüzey sertliğinin ve bağlanma dayanımının araştırılması [tez]. Konya: Selçuk Üniversitesi; 2008.
  • Büyüközer T. Farklı ışık kaynakları ile polimerize edilen çeşitli adeziv sistemlerden farklı dentin kalınlıkları altında salınan artık monomerlerin yüksek basınçlı likit kromatografisi ile analizi [tez]. Konya: Selçuk Üniversitesi; 2009.
  • Schilke R, Lisson JA, Bauss O, Geurtsen W. Comparison of the number and diameter of dentinal tubules in human and bovine dentine by scanning electron microscopic investigation. Arch Oral Biol 2000;45:355-61.
  • Altıntaş S, Usumez A. Evaluation of monomer leaching from a dual cured resin cement. J Biomed Mater Res Part B: Appl Biomater 2008;86:523-9.
  • Söderholm KJ, Mariotti A. BIS-GMA--based resins in dentistry: are they safe? J Am Dent Assoc 1999;130:201-9.
  • Schmalz G. The biocompatiblity of non-amalgam dental filling materials. Eur J Oral Sci 1998;106:696-706.
There are 26 citations in total.

Details

Primary Language Turkish
Subjects Health Care Administration
Journal Section Original Research Article
Authors

Mustafa Altunsoy

Murat Selim Botsalı This is me

Gonca Tosun This is me

Ahmet Yaşar This is me

Publication Date March 11, 2013
Published in Issue Year 2013 Volume: 30 Issue: 1

Cite

APA Altunsoy, M., Botsalı, M. S., Tosun, G., Yaşar, A. (2013). Farklı polimerizasyon sürelerinin adeziv sistemlerden salınan artık monomer miktarına etkisi. Acta Odontologica Turcica, 30(1), 6-12.
AMA Altunsoy M, Botsalı MS, Tosun G, Yaşar A. Farklı polimerizasyon sürelerinin adeziv sistemlerden salınan artık monomer miktarına etkisi. Acta Odontol Turc. April 2013;30(1):6-12.
Chicago Altunsoy, Mustafa, Murat Selim Botsalı, Gonca Tosun, and Ahmet Yaşar. “Farklı Polimerizasyon sürelerinin Adeziv Sistemlerden salınan artık Monomer miktarına Etkisi”. Acta Odontologica Turcica 30, no. 1 (April 2013): 6-12.
EndNote Altunsoy M, Botsalı MS, Tosun G, Yaşar A (April 1, 2013) Farklı polimerizasyon sürelerinin adeziv sistemlerden salınan artık monomer miktarına etkisi. Acta Odontologica Turcica 30 1 6–12.
IEEE M. Altunsoy, M. S. Botsalı, G. Tosun, and A. Yaşar, “Farklı polimerizasyon sürelerinin adeziv sistemlerden salınan artık monomer miktarına etkisi”, Acta Odontol Turc, vol. 30, no. 1, pp. 6–12, 2013.
ISNAD Altunsoy, Mustafa et al. “Farklı Polimerizasyon sürelerinin Adeziv Sistemlerden salınan artık Monomer miktarına Etkisi”. Acta Odontologica Turcica 30/1 (April 2013), 6-12.
JAMA Altunsoy M, Botsalı MS, Tosun G, Yaşar A. Farklı polimerizasyon sürelerinin adeziv sistemlerden salınan artık monomer miktarına etkisi. Acta Odontol Turc. 2013;30:6–12.
MLA Altunsoy, Mustafa et al. “Farklı Polimerizasyon sürelerinin Adeziv Sistemlerden salınan artık Monomer miktarına Etkisi”. Acta Odontologica Turcica, vol. 30, no. 1, 2013, pp. 6-12.
Vancouver Altunsoy M, Botsalı MS, Tosun G, Yaşar A. Farklı polimerizasyon sürelerinin adeziv sistemlerden salınan artık monomer miktarına etkisi. Acta Odontol Turc. 2013;30(1):6-12.