Research Article
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Year 2022, Volume: 14 Issue: 2, 24 - 29, 29.12.2022
https://doi.org/10.56484/iamr.1162647

Abstract

References

  • 1- Sıegel Rl, Mıller Kd, Jemal A. Cancer statistics,. CA Cancer J Clin, 2016; 66:7–30.
  • 2- Das V, Kalıta J, Pal M. Predictive and prognostic biomarkers in colorectal cancer: A systematic review of recent advances and challenges. Biomedicine & Pharmacotherapy, (2017);87:8–19.
  • 3- Kehoe SI, Jayarao BM, Heinrichs AJ. A Survey of Bovine Colostrum Composition and Colostrum Management Practices on Pennsylvania Dairy farms. Journal of Medical Sci. 2007;90(9):4108–4116.
  • 4- Kleinsmith Ba. Bovıne Colostrum As A Resource For The Powerful Antıoxıdant Glutathıone. Journal of Medical Sci. 2009;13-25.
  • 5- Przybylska J, Albera E, Kankofer M. Antioxidants in Bovine Colostrum. Reprod Domest Anim. 2007;42(4):402–419.
  • 6- Kelly GS. Bovine Colostrums: A Review of Clinical Uses. Journal of Medical Sci. 2003;8(4):378–394.
  • 7- Lascelles AK. The İmmune System on The Ruminant Mammary Gland and its Role in The Control of Mastitis. Journal of Medical Sci. 1979;62(1):154–167.
  • 8- Argüello A, Castro N, Batista M, Moreno-Indias I, Morales-delaNuez A, Sanchez-Macias D. Chitotriosidase Activity in Goat Blood and Colostrum. Journal of Medical Sci. 2008;91(5):2067–2070.
  • 9- Erdem H, Atasever Ş. Yeni Doğan Buzağılarda Kolostrumun Önemi. Ondokuzmayıs Üniv Zir Fak Derg. 2005(2):79-84.
  • 10- Kittaneh M, Montero AJ, Glück S. Molecular Profiling for Breast Cancer: A Comprehensive Review. 2013;(5):61–70.
  • 11- Rossi A, Caracciolo V, Russo G, Reiss K, Giordano A. Medulloblastoma: from Molecular Pathology to Therapy. National Library of Medicine. 2008;14(4):971-986. 12- Karag C. Peyniraltı Suyu Proteinlerinin Fonksiyonel Özellikleri ve Sağlık Üzerine Etkileri. Ege Üniversitesi Ziraat Fakültesi Derg. 2004;41(2):197–207. 13- Bounous G, Papenburg R, Kongshavn PA, Gold P, Fleiszer D. Dietary Whey Protein İnhibits The Development of Dimethylhydrazine İnduced Malignancy. Journal of Medical Sci. 1988;11(3):213–227.
  • 14- Fakharany E.M, Abu-serie M, Litus E.A, Permyakov S.E, Uversky V.N, Redwan E.M. The Use of Human, Cow, and Cmel Milk Albumins in Anticancer Comlexes with Oleic Asid. The Protein Journal, 2018;(37):203-215.
  • 15- McIntosh GH, Regester GO, Le Leu RK, Royle PJ. Smithers GW Dairy proteins protect against dimethylhydrazineinduced intestinal cancers in rats. Journal of Nutrition. 1995;(125):809–816.
  • 16- Karagözlü C, Bayerer M. Peyniraltı Suyu Proteinlerinin Fonksiyonel Özellikleri Ve Sağlık Üzerine Etkileri. Ege Üniv. Ziraat Fak. Dergisi, 2004;41(2):197-207.

Cytoxic Effect Levels Of Sheep Whey Protein in Colorectal Adenocarcinoma Cell Line (Caco-2)

Year 2022, Volume: 14 Issue: 2, 24 - 29, 29.12.2022
https://doi.org/10.56484/iamr.1162647

Abstract

Abstract
Objective: It was aimed to determine the antitumor and antiproliferative activity of whey protein (whey) obtained from sheep colostrum isolation on colorectal cancer cells (Caco-2).
Method: Colostrum was separated into fractions as whey proteins using the isoelectric point. Before cell culture was studied, whey protein was sterilized by a membrane filter. Antitumoral activity levels of different concentrations of lyophilized proteins were measured by MTT viability test on cells and IC50 values were determined.
Results: Sheep whey proteins were incubated with Caco-2 cells for 24, 48 and 72 hours with serial dilutions starting with 3200 µg/ml and decreasing. When incubated with sheep whey Caco-2 cells, cell viability: 66.34% at 24 h; IC50 value of 8.414 µg/ml; IC50 value of 46.61% at 48 h was 5,838 µg/ml; The IC50 value of 4.61% at 72 h was found to be 4,741 µg/ml.
Conclusion: It was concluded that whey proteins obtained from sheep colostrum isolation have apoptotic and anticarcinogenic effects on Caco-2 cancer cell line and significantly inhibit the growth of tumor cells. We think that the results of this study will lead to studies to be carried out in vitro.

References

  • 1- Sıegel Rl, Mıller Kd, Jemal A. Cancer statistics,. CA Cancer J Clin, 2016; 66:7–30.
  • 2- Das V, Kalıta J, Pal M. Predictive and prognostic biomarkers in colorectal cancer: A systematic review of recent advances and challenges. Biomedicine & Pharmacotherapy, (2017);87:8–19.
  • 3- Kehoe SI, Jayarao BM, Heinrichs AJ. A Survey of Bovine Colostrum Composition and Colostrum Management Practices on Pennsylvania Dairy farms. Journal of Medical Sci. 2007;90(9):4108–4116.
  • 4- Kleinsmith Ba. Bovıne Colostrum As A Resource For The Powerful Antıoxıdant Glutathıone. Journal of Medical Sci. 2009;13-25.
  • 5- Przybylska J, Albera E, Kankofer M. Antioxidants in Bovine Colostrum. Reprod Domest Anim. 2007;42(4):402–419.
  • 6- Kelly GS. Bovine Colostrums: A Review of Clinical Uses. Journal of Medical Sci. 2003;8(4):378–394.
  • 7- Lascelles AK. The İmmune System on The Ruminant Mammary Gland and its Role in The Control of Mastitis. Journal of Medical Sci. 1979;62(1):154–167.
  • 8- Argüello A, Castro N, Batista M, Moreno-Indias I, Morales-delaNuez A, Sanchez-Macias D. Chitotriosidase Activity in Goat Blood and Colostrum. Journal of Medical Sci. 2008;91(5):2067–2070.
  • 9- Erdem H, Atasever Ş. Yeni Doğan Buzağılarda Kolostrumun Önemi. Ondokuzmayıs Üniv Zir Fak Derg. 2005(2):79-84.
  • 10- Kittaneh M, Montero AJ, Glück S. Molecular Profiling for Breast Cancer: A Comprehensive Review. 2013;(5):61–70.
  • 11- Rossi A, Caracciolo V, Russo G, Reiss K, Giordano A. Medulloblastoma: from Molecular Pathology to Therapy. National Library of Medicine. 2008;14(4):971-986. 12- Karag C. Peyniraltı Suyu Proteinlerinin Fonksiyonel Özellikleri ve Sağlık Üzerine Etkileri. Ege Üniversitesi Ziraat Fakültesi Derg. 2004;41(2):197–207. 13- Bounous G, Papenburg R, Kongshavn PA, Gold P, Fleiszer D. Dietary Whey Protein İnhibits The Development of Dimethylhydrazine İnduced Malignancy. Journal of Medical Sci. 1988;11(3):213–227.
  • 14- Fakharany E.M, Abu-serie M, Litus E.A, Permyakov S.E, Uversky V.N, Redwan E.M. The Use of Human, Cow, and Cmel Milk Albumins in Anticancer Comlexes with Oleic Asid. The Protein Journal, 2018;(37):203-215.
  • 15- McIntosh GH, Regester GO, Le Leu RK, Royle PJ. Smithers GW Dairy proteins protect against dimethylhydrazineinduced intestinal cancers in rats. Journal of Nutrition. 1995;(125):809–816.
  • 16- Karagözlü C, Bayerer M. Peyniraltı Suyu Proteinlerinin Fonksiyonel Özellikleri Ve Sağlık Üzerine Etkileri. Ege Üniv. Ziraat Fak. Dergisi, 2004;41(2):197-207.
There are 14 citations in total.

Details

Primary Language English
Subjects Clinical Sciences
Journal Section Original Research Paper
Authors

İlhan Sabancılar 0000-0002-0773-2752

Publication Date December 29, 2022
Published in Issue Year 2022 Volume: 14 Issue: 2

Cite

APA Sabancılar, İ. (2022). Cytoxic Effect Levels Of Sheep Whey Protein in Colorectal Adenocarcinoma Cell Line (Caco-2). International Archives of Medical Research, 14(2), 24-29. https://doi.org/10.56484/iamr.1162647
AMA Sabancılar İ. Cytoxic Effect Levels Of Sheep Whey Protein in Colorectal Adenocarcinoma Cell Line (Caco-2). IAMR. December 2022;14(2):24-29. doi:10.56484/iamr.1162647
Chicago Sabancılar, İlhan. “Cytoxic Effect Levels Of Sheep Whey Protein in Colorectal Adenocarcinoma Cell Line (Caco-2)”. International Archives of Medical Research 14, no. 2 (December 2022): 24-29. https://doi.org/10.56484/iamr.1162647.
EndNote Sabancılar İ (December 1, 2022) Cytoxic Effect Levels Of Sheep Whey Protein in Colorectal Adenocarcinoma Cell Line (Caco-2). International Archives of Medical Research 14 2 24–29.
IEEE İ. Sabancılar, “Cytoxic Effect Levels Of Sheep Whey Protein in Colorectal Adenocarcinoma Cell Line (Caco-2)”, IAMR, vol. 14, no. 2, pp. 24–29, 2022, doi: 10.56484/iamr.1162647.
ISNAD Sabancılar, İlhan. “Cytoxic Effect Levels Of Sheep Whey Protein in Colorectal Adenocarcinoma Cell Line (Caco-2)”. International Archives of Medical Research 14/2 (December 2022), 24-29. https://doi.org/10.56484/iamr.1162647.
JAMA Sabancılar İ. Cytoxic Effect Levels Of Sheep Whey Protein in Colorectal Adenocarcinoma Cell Line (Caco-2). IAMR. 2022;14:24–29.
MLA Sabancılar, İlhan. “Cytoxic Effect Levels Of Sheep Whey Protein in Colorectal Adenocarcinoma Cell Line (Caco-2)”. International Archives of Medical Research, vol. 14, no. 2, 2022, pp. 24-29, doi:10.56484/iamr.1162647.
Vancouver Sabancılar İ. Cytoxic Effect Levels Of Sheep Whey Protein in Colorectal Adenocarcinoma Cell Line (Caco-2). IAMR. 2022;14(2):24-9.

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