Research Article
Treatment of Burn Wounds with a Chitosan-Based Hydrogel Dressing Containing Artemisia absinthium L.: A Comprehensive In Vivo Study
Abstract
Objective: Wormwood (Artemisia absinthium L.) is a valuable medicinal plant that has been used for the wound healing since ancient times. Hydrogel dressings are often preferred for wound care in treatment. In this study, the effects of chitosan-based hydrogel dressings containing wormwood were investigated in rats with burn wound model for the first time.
Material-Method: Certain phenolic compounds in wormwood extracts were detected by LC-MS/MS and antioxidant activities were calculated using the DPPH. The antimicrobial activity of the dressings was tested using the disk diffusion method. Tissues taken from 48 female Sprague-Dawley rats were histopathologically examined at day 3rd and 21st. Skin tissue cytokine levels were measured using ELISA. All data obtained from histopathological examination and cytokine levels were statistically evaluated.
Results: Fifteen phenolic compounds were quantitatively determined in wormwood extracts. The antioxidant activities of high-, medium-, and low-dose wormwood extracts were as 91,1% ± 0,054, 89,6% ± 0,012 and 84,1% ± 0,02, respectively. The hydrogel dressings did not show any antimicrobial activity against S. aureus (ATCC 29213) or P. aeruginosa (ATCC 27853). Granulation tissue formation, collagen increase, and regular scar appearance were higher in the all three wormwood groups. Wound contraction was completed and remodeling phase started at day 21st, especially in the high-dose wormwood treatment group. Tissue cytokine levels were determined in pg/ml in all groups.
Conclusion: It was determined that A. absinthium L. can promote wound healing through various mechanisms of action and shows immunomodulatory effects, and is appropriate for use as a wound dressing in the form of a chitosan-based hydrogel.
Keywords
Ethical Statement
This research was approved by the Düzce University Local Ethics Committe Presidency On Animal Testings with decisions number 2022/06/08.
Supporting Institution
Düzce University
Project Number
Düzce University (BAP-2021.04.03.1220)
References
- Jeschke MG, van Baar ME, Choudhry MA, Chung KK, Gibran NS, Logsetty S. Burn injury. Nat Rev Dis Prim. 2020;6(1).
- Hao D, Nourbakhsh M. Recent advances in experimental burn models. Biology (Basel). 2021;10(6).
- Markiewicz-Gospodarek A, Kozioł M, Tobiasz M, Baj J, Radzikowska-Büchner E, Przekora A. Burn wound healing: Clinical complications, medical care, treatment, and dressing types: The current state of knowledge for clinical practice. Int J Environ Res Public Health. 2022;19(3):1338.
- Johnson RM, Richard R. Partial-thickness burns: identification and management. Adv Skin Wound Care. 2003;16(4):178-187.
- Tiwari VK. Burn wound: How it differs from other wounds. Indian J Plast Surg. 2012;45(2):364-373.
- Chester DL, Papini RPG. Skin and skin substitutes in burn management. Trauma. 2004;6(2):87-99.
- Liu HF, Zhang F, Lineaweaver WC. History and advancement of burn treatments. Ann Plast Surg. 2017;78(2):S2-S8.
- Fan P, Zeng Y, Zaldivar-Silva D, Agüero L, Wang S. Chitosan-Based Hemostatic Hydrogels: The Concept, Mechanism, Application, and Prospects. Molecules. 2023;28(3).
- Paul W, Sharma CP. Chitosan and alginate wound dressings : A short review. Trends Biometerials Artif Organs. 2004;18(1):18-23.
- Dhivya S, Padma VV, Santhini E. Wound dressings - A review. Biomed. 2015;5(4):24-28.
- Goud BJ, Dwarakanath.V, B.K.Chikka S. Α Review on History, Controversy, Traditional Use, Ethnobotany, Phytochemistry and Pharmacology of Artemisia Absinthium Linn. Adv Res Eng Appl Sci. 2015;4(5):77-107.
- Koul B, Taak P. The Artemisia genus: A review on traditional uses, phytochemical constituents, pharmacological properties and germplasm conservation. J Glycomics Lipidomics. 2018;07(01).
- Lachenmeier DW. Wormwood (Artemisia absinthium L.)-A curious plant with both neurotoxic and neuroprotective properties? J Ethnopharmacol. 2010;131(1):224-227.
- Uzun E, Sariyar G, Adsersen A, et al. Traditional medicine in Sakarya province (Turkey) and antimicrobial activities of selected species. J Ethnopharmacol. 2004;95(2-3):287-296.
- Kültür Ş. Medicinal plants used in Kirklareli Province (Turkey). J Ethnopharmacol. 2007;111(2):341-364.
- Sile I, Romane E, Reinsone S, Maurina B, Tirzite D, Dambrova M. Medicinal plants and their uses recorded in the Archives of Latvian Folklore from the 19th century. J Ethnopharmacol. 2020;249:112378.
- Syal S, Pandit V, Ashawat MS. Traditional herbs to treat acne vulgaris. Asian J Pharm Res. 2020;10(3):195.
- Yarnell E, Abascal K. Herbal medicine for acne vulgaris. Altern Complement Ther. 2006;12(6):303-309.
- Moacã EA, Pavel IZ, Danciu C, et al. Romanian wormwood (Artemisia absinthium L.): Physicochemical and nutraceutical screening. Molecules. 2019;24(17).
- Lee YJ, Thiruvengadam M, Chung IM, Nagella P. Polyphenol composition and antioxidant activity from the vegetable plant Artemisia absinthium L. Aust J Crop Sci. 2013;7(12):1921-1926.
- Koyuncu I. Evaluation of anticancer, antioxidant activity and phenolic compounds of Artemisia absinthium L. Extract. Cell Mol Biol. 2018;6(3):25-34.
- Hoseinian A, Moslemi HR, Sedaghat R. Antioxidant properties of Artemisia absinthium accelerate healing of experimental Achilles tendon injury in rabbits. Herba Pol. 2018;64(1):36-43.
- Minda D, Ghiulai R, Banciu CD, et al. Phytochemical Profile, Antioxidant and Wound Healing Potential of Three Artemisia Species: In Vitro and In Ovo Evaluation. Appl Sci. 2022;12(3):1359.
- Baykan Erel Ş, Reznicek G, Şenol SG, Karabay Yavaşoǧulu NÜ, Konyalioǧlu S, Zeybek AU. Antimicrobial and antioxidant properties of Artemisia L. species from western anatolia. Turkish J Biol. 2012;36(1):75-84.
- Erdoǧrul ÖT. Antibacterial activities of some plant extracts used in folk medicine. Pharm Biol. 2002;40(4):269-273.
- Hrytsyk RA, Kutsyk RV, Yurchyshyn OI, Struk ОА, Kireev IV, Grytsyk AR. The investigation of antimicrobial and antifungal activity of some Artemisia L. species. Pharmacia. 2021;68(1):93-100.
- Sultan MH, Zuwaiel AA, Moni SS, et al. Bioactive Principles and Potentiality of Hot Methanolic Extract of the Leaves from Artemisia absinthium L “in vitro Cytotoxicity Against Human MCF-7 Breast Cancer Cells, Antibacterial Study and Wound Healing Activity.” Curr Pharm Biotechnol. 2020;21(15):1711-1721.
- Benkhaled A, Boudjelal A, Napoli E, Baali F, Ruberto G. Phytochemical profile, antioxidant activity and wound healing properties of Artemisia absinthium essential oil. Asian Pac J Trop Biomed. 2020;10(11):496-504.
- Boudjelal A, Smeriglio A, Ginestra G, Denaro M, Trombetta D. Phytochemical profile, safety assessment and wound healing activity of artemisia absinthium L. Plants. 2020;9(12):1-14.
Year 2024,
Volume: 5 Issue: 1, 54 - 64, 29.04.2024
Abstract
Project Number
Düzce University (BAP-2021.04.03.1220)
References
- Jeschke MG, van Baar ME, Choudhry MA, Chung KK, Gibran NS, Logsetty S. Burn injury. Nat Rev Dis Prim. 2020;6(1).
- Hao D, Nourbakhsh M. Recent advances in experimental burn models. Biology (Basel). 2021;10(6).
- Markiewicz-Gospodarek A, Kozioł M, Tobiasz M, Baj J, Radzikowska-Büchner E, Przekora A. Burn wound healing: Clinical complications, medical care, treatment, and dressing types: The current state of knowledge for clinical practice. Int J Environ Res Public Health. 2022;19(3):1338.
- Johnson RM, Richard R. Partial-thickness burns: identification and management. Adv Skin Wound Care. 2003;16(4):178-187.
- Tiwari VK. Burn wound: How it differs from other wounds. Indian J Plast Surg. 2012;45(2):364-373.
- Chester DL, Papini RPG. Skin and skin substitutes in burn management. Trauma. 2004;6(2):87-99.
- Liu HF, Zhang F, Lineaweaver WC. History and advancement of burn treatments. Ann Plast Surg. 2017;78(2):S2-S8.
- Fan P, Zeng Y, Zaldivar-Silva D, Agüero L, Wang S. Chitosan-Based Hemostatic Hydrogels: The Concept, Mechanism, Application, and Prospects. Molecules. 2023;28(3).
- Paul W, Sharma CP. Chitosan and alginate wound dressings : A short review. Trends Biometerials Artif Organs. 2004;18(1):18-23.
- Dhivya S, Padma VV, Santhini E. Wound dressings - A review. Biomed. 2015;5(4):24-28.
- Goud BJ, Dwarakanath.V, B.K.Chikka S. Α Review on History, Controversy, Traditional Use, Ethnobotany, Phytochemistry and Pharmacology of Artemisia Absinthium Linn. Adv Res Eng Appl Sci. 2015;4(5):77-107.
- Koul B, Taak P. The Artemisia genus: A review on traditional uses, phytochemical constituents, pharmacological properties and germplasm conservation. J Glycomics Lipidomics. 2018;07(01).
- Lachenmeier DW. Wormwood (Artemisia absinthium L.)-A curious plant with both neurotoxic and neuroprotective properties? J Ethnopharmacol. 2010;131(1):224-227.
- Uzun E, Sariyar G, Adsersen A, et al. Traditional medicine in Sakarya province (Turkey) and antimicrobial activities of selected species. J Ethnopharmacol. 2004;95(2-3):287-296.
- Kültür Ş. Medicinal plants used in Kirklareli Province (Turkey). J Ethnopharmacol. 2007;111(2):341-364.
- Sile I, Romane E, Reinsone S, Maurina B, Tirzite D, Dambrova M. Medicinal plants and their uses recorded in the Archives of Latvian Folklore from the 19th century. J Ethnopharmacol. 2020;249:112378.
- Syal S, Pandit V, Ashawat MS. Traditional herbs to treat acne vulgaris. Asian J Pharm Res. 2020;10(3):195.
- Yarnell E, Abascal K. Herbal medicine for acne vulgaris. Altern Complement Ther. 2006;12(6):303-309.
- Moacã EA, Pavel IZ, Danciu C, et al. Romanian wormwood (Artemisia absinthium L.): Physicochemical and nutraceutical screening. Molecules. 2019;24(17).
- Lee YJ, Thiruvengadam M, Chung IM, Nagella P. Polyphenol composition and antioxidant activity from the vegetable plant Artemisia absinthium L. Aust J Crop Sci. 2013;7(12):1921-1926.
- Koyuncu I. Evaluation of anticancer, antioxidant activity and phenolic compounds of Artemisia absinthium L. Extract. Cell Mol Biol. 2018;6(3):25-34.
- Hoseinian A, Moslemi HR, Sedaghat R. Antioxidant properties of Artemisia absinthium accelerate healing of experimental Achilles tendon injury in rabbits. Herba Pol. 2018;64(1):36-43.
- Minda D, Ghiulai R, Banciu CD, et al. Phytochemical Profile, Antioxidant and Wound Healing Potential of Three Artemisia Species: In Vitro and In Ovo Evaluation. Appl Sci. 2022;12(3):1359.
- Baykan Erel Ş, Reznicek G, Şenol SG, Karabay Yavaşoǧulu NÜ, Konyalioǧlu S, Zeybek AU. Antimicrobial and antioxidant properties of Artemisia L. species from western anatolia. Turkish J Biol. 2012;36(1):75-84.
- Erdoǧrul ÖT. Antibacterial activities of some plant extracts used in folk medicine. Pharm Biol. 2002;40(4):269-273.
- Hrytsyk RA, Kutsyk RV, Yurchyshyn OI, Struk ОА, Kireev IV, Grytsyk AR. The investigation of antimicrobial and antifungal activity of some Artemisia L. species. Pharmacia. 2021;68(1):93-100.
- Sultan MH, Zuwaiel AA, Moni SS, et al. Bioactive Principles and Potentiality of Hot Methanolic Extract of the Leaves from Artemisia absinthium L “in vitro Cytotoxicity Against Human MCF-7 Breast Cancer Cells, Antibacterial Study and Wound Healing Activity.” Curr Pharm Biotechnol. 2020;21(15):1711-1721.
- Benkhaled A, Boudjelal A, Napoli E, Baali F, Ruberto G. Phytochemical profile, antioxidant activity and wound healing properties of Artemisia absinthium essential oil. Asian Pac J Trop Biomed. 2020;10(11):496-504.
- Boudjelal A, Smeriglio A, Ginestra G, Denaro M, Trombetta D. Phytochemical profile, safety assessment and wound healing activity of artemisia absinthium L. Plants. 2020;9(12):1-14.
There are 29 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Traditional, Complementary and Integrative Medicine (Other) |
| Journal Section | Research Articles |
| Authors | |
| Project Number | Düzce University (BAP-2021.04.03.1220) |
| Publication Date | April 29, 2024 |
| Submission Date | February 20, 2024 |
| Acceptance Date | April 16, 2024 |
| Published in Issue | Year 2024 Volume: 5 Issue: 1 |
Cite
This journal is licensed under a Creative Commons Attribution 4.0 International License.