Çörek Otu (Nigella sativa) Yağının Trichomonas vaginalis’e Karşı in vitro Anti-trikomoniyaz Etkisinin Araştırılması
Abstract
Çörek Otu (Nigella sativa) Yağının Trichomonas vaginalis’e Karşı in vitro Anti-trikomoniyaz Etkisinin Araştırılması
Özet
- Amaç: Trikomoniyazın güncel tedavisinde 5-nitroimidazol türevleri (metronidazol,ornidazol) kullanılmasına rağmen, Trichomonas vaginalis türlerinin metronidazole karşı direnç geliştirdiği yaklaşık 50 yıldır bilinmektedir. Bu nedenle trikomoniyaz tedavisinde uzun süredir alternatif tedavi yöntemleri araştırılmaktadır. Bu çalışmada çörek otu yağının Trichomonas vaginalis’e karşı in vitro etkisinin araştırılması amaçlanmıştır. Gereç ve Yöntem: Çörek otu yağı ve metronidazolün T. vaginalis’e karşı etkinliğinin araştırılması işlemi 24 kuyucuklu hücre kültürü plaklarında dilüsyon yöntemiyle gerçekleştirilmiştir.Bu işlem için kısaca, T. vaginalis trofozoitleri TYM besiyeri içeren hücre kültürü plaklarına alınmıştır. Daha sonra, çörek otu yağı ve metronidazolün farklı konsantrasyonları plaklara eklenmiş ve 37°C’de inkübe edilmişlerdir. Bulgular: İnkübasyonun 48. saatinde, 250 μg/ml konsantrasyonda hareketli trofozoitlerin görülmüş olmasına rağmen 500 μg/ml, 1000 μg/ml ve 2000 μl/ml konsantrasyonlarda hareket gözlenmemiştir. 72. saatte, 250 μl/mg konsantrasyonu hariç geriye kalan tüm konsantrasyonlarda hareket görülmemesine ek olarak hücre lizisi görülmüştür. Ayrıca 72. saat sonunda minimum inhibitör konsantrasyon (MİK) 500μl/mg iken, minimum lethal konsantrasyon (MLK) 1000 μl/mg’dir. Sonuç: Bu çalışmada çörek otu yağı, trikomoniyazis tedavisi için alternatif bir terapötik ajan olarak in vitro ortamda denenmiş ve trikomoniyaz tedavisinde doğal önemli bir tedavi edici potansiyele sahip olduğu bulunmuştur. Bundan sonraki çalışmalarda daha ileri in vivo araştırmaların yapılması ve aktiviteden sorumlu olabilecek bileşenlerin tespiti planlanmaktadır.
Anahtar
Kelimeler: (3-5 Kelime): Trichomonas vaginalis, nigella sativa, anti-trikomoniyaz etki
Investigation of in vitro Anti-trichomoniasis Effect of Nigella Sativa Oil against Trichomonas vaginalis
Abstract
Objective: Although 5-nitroimidazole derivatives (metronidazole, ornidazole) are being used in the current treatment of trichomoniasis, it has been known the presence of MTZ-resistant T. vaginalis strains for almost 50 years. Therefore, alternative treatment methods are being investigated for a long time for treatment of trichomoniasis. In this study, it was aimed to investigate in vitro activity of Nigella sativa oil against Trichomonas vaginalis. Material and Methods: In vitro testing of Nigella sativa and metronidazole against T. vaginalis was performed in cell culture plate by dilution method. Briefly, T. vaginalis trophozoites were transferred to 24-well plate containing TYM medium. Thereafter, different concentrations of Nigella sativa and metronidazole were added to each well and incubated at 37°C. Findings: At 48th hour of incubation, although motile trophozoites at 250 μg/ml concentration of Nigella sativa oil were observed, immotile trophozoites were detected at 500 μg/ml, 1000 μg/ml and 2000 μl/ml concentrations of Nigella sativa oil. At 72th hour, immotile trophozoites as well as trophozoite lysis were detected in all concentration of Nigella sativa oil, except for 250 μg/ ml concentration. Also at the end of 72th hour for Nigella sativa, MIC was 500 μl/mg while MLK was 1000μl/
mg. Conclusion: In this study, Nigella sativa oil was tested in vitro as an alternative therapeutic agent for treatment of trichomoniasis and it has been found that Nigella sativa oil has a naturally important therapeutic potential for trichomoniasis treatment. In vivo tests and the isolation and identification of compounds responsible for the detected activity are planned for further investigations
Keywords:
(3-5
Words): Trichomonas vaginalis, nigella sativa, anti-trichomoniasis effect
References
- Al-Homidan, A., Al-Qarawi, A.A., Al-Waily, S.A., & Adam, S.E. (2002). Response of broiler chicks to dietary Rhazyastricta and Nigella sativa. Br Poult Sci, 43, 291–296.
- Aminou, H.A., Alam-Eldin, Y.H., & Hashem, H.A. (2016). Effect of Nigella sativa alcoholic extract and oil, as well as Phaseolus vulgaris (kidney bean) lectin on the ultrastructure of Trichomonas vaginalis trophozoites. J Parasit Dis, 40(3), 707-713.
- Badary, O.A., Al-Shabanah, O.A., Nagi, M.N., Al-Bekairi, A.M., & Elmazar, M.A. (1998). Acute and subchronic toxicity of thymoquinone in mice. Drug Dev Res, 44, 56–61.
- Bishara, S.A., & Masoud, S.I. (1992). Effect of Nigella sativa extract on experimental giardiasis. New Egypt J Med, 7, 1–3.
- El Wakil HS. (2007). Evaluation of in vitro effect of Nigella sativa aqueous extract on Blastocystis hominis isolates. J Egypt Soc Parasitol, 37(3), 801–813.
- Gökmen, A.A., Girginkardeşler, N., Kilimcioğlu, A.A., Şirin, M.C., & Özbilgin, A. (2016). Trichomonas vaginalis’in Metronidazol, Ornidazolve Proton Pompa İnhibitörleri Pantoprazol ve Esomeprazole Karşı İn Vitro Duyarlılığı. Mikrobiyol Bul, 50(1), 133-139.
- Inceboz, T., Inceboz, U., & Öztürk, S. (2004). Comparative In vitro Cytotoxic Effects of Ornidazole, Metronidazole and Ciprofloxacin against Trichomonas vaginalis Trophozoites. J Chemother, 16(5), 459-62.
- Kaya, M.S., Kara, M., & Özbek, H. (2003). Çörek Otu (Nigella Sativa) Tohumunun İnsan Hücresel Bağışıklık Sisteminin CD3+, CD4+, CD8+ Hücreleri Ve Toplam Lökosit Sayisi Üzerine Etkileri. Genel Tıp Derg, 13(3), 109-112.
- Mona Abd El-Fattah, M.A., Mahmoud, A., Aminou, H.A.K., & Hashem, H.A. (2016). Are the fatty acids responsible for the higher effect of oiland alcoholic extract of Nigella sativa over its aqueous extracton Trichomonas vaginalis trophozoites. J Parasit Dis, 40(1), 22–31.
- Özcel MA, Zeyrek FY. Trikomoniyaz. In: Özcel’in Tıbbi Paraziter Hastalıkları. Özcel A, Özbel Y, Ak M, eds. Türkiye Parazitoloji Derneği Yayın No: 22. İzmir, 2007; s. 431-447.
- Shaaban, M.T., El Silk, S.E., & Tayel, M.A. (2011). Efficiency of some plant extracts, carbohydrates and inorganic salts as anti-adhesio nagents against the adhesion of Staphylococcus strains to HEp-2cells. Life Sci J, 8, 1172–1182.
- Tennekoon, K., Jeevathayaparan, S., & Kurukulasooriya, A. (1991). (Karunanayake. Possible hepatotoxicity of Nigella sativa seeds and Dregea volubilis leaves. J Ethnopharmacol, 31, 283–289.
Abstract
References
- Al-Homidan, A., Al-Qarawi, A.A., Al-Waily, S.A., & Adam, S.E. (2002). Response of broiler chicks to dietary Rhazyastricta and Nigella sativa. Br Poult Sci, 43, 291–296.
- Aminou, H.A., Alam-Eldin, Y.H., & Hashem, H.A. (2016). Effect of Nigella sativa alcoholic extract and oil, as well as Phaseolus vulgaris (kidney bean) lectin on the ultrastructure of Trichomonas vaginalis trophozoites. J Parasit Dis, 40(3), 707-713.
- Badary, O.A., Al-Shabanah, O.A., Nagi, M.N., Al-Bekairi, A.M., & Elmazar, M.A. (1998). Acute and subchronic toxicity of thymoquinone in mice. Drug Dev Res, 44, 56–61.
- Bishara, S.A., & Masoud, S.I. (1992). Effect of Nigella sativa extract on experimental giardiasis. New Egypt J Med, 7, 1–3.
- El Wakil HS. (2007). Evaluation of in vitro effect of Nigella sativa aqueous extract on Blastocystis hominis isolates. J Egypt Soc Parasitol, 37(3), 801–813.
- Gökmen, A.A., Girginkardeşler, N., Kilimcioğlu, A.A., Şirin, M.C., & Özbilgin, A. (2016). Trichomonas vaginalis’in Metronidazol, Ornidazolve Proton Pompa İnhibitörleri Pantoprazol ve Esomeprazole Karşı İn Vitro Duyarlılığı. Mikrobiyol Bul, 50(1), 133-139.
- Inceboz, T., Inceboz, U., & Öztürk, S. (2004). Comparative In vitro Cytotoxic Effects of Ornidazole, Metronidazole and Ciprofloxacin against Trichomonas vaginalis Trophozoites. J Chemother, 16(5), 459-62.
- Kaya, M.S., Kara, M., & Özbek, H. (2003). Çörek Otu (Nigella Sativa) Tohumunun İnsan Hücresel Bağışıklık Sisteminin CD3+, CD4+, CD8+ Hücreleri Ve Toplam Lökosit Sayisi Üzerine Etkileri. Genel Tıp Derg, 13(3), 109-112.
- Mona Abd El-Fattah, M.A., Mahmoud, A., Aminou, H.A.K., & Hashem, H.A. (2016). Are the fatty acids responsible for the higher effect of oiland alcoholic extract of Nigella sativa over its aqueous extracton Trichomonas vaginalis trophozoites. J Parasit Dis, 40(1), 22–31.
- Özcel MA, Zeyrek FY. Trikomoniyaz. In: Özcel’in Tıbbi Paraziter Hastalıkları. Özcel A, Özbel Y, Ak M, eds. Türkiye Parazitoloji Derneği Yayın No: 22. İzmir, 2007; s. 431-447.
- Shaaban, M.T., El Silk, S.E., & Tayel, M.A. (2011). Efficiency of some plant extracts, carbohydrates and inorganic salts as anti-adhesio nagents against the adhesion of Staphylococcus strains to HEp-2cells. Life Sci J, 8, 1172–1182.
- Tennekoon, K., Jeevathayaparan, S., & Kurukulasooriya, A. (1991). (Karunanayake. Possible hepatotoxicity of Nigella sativa seeds and Dregea volubilis leaves. J Ethnopharmacol, 31, 283–289.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Araştırma Makaleleri |
| Authors | |
| Publication Date | October 5, 2018 |
| Submission Date | February 5, 2018 |
| Published in Issue | Year 2018 Volume: 3 Issue: 3 |
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