Research Article
BibTex RIS Cite

Midline ve transvers laparotomi sonrası yara iyileşmesinde oluşan kollajen türleri farklı mıdır? Farelerde deneysel bir çalışma

Year 2021, Volume: 5 Issue: 4, 331 - 335, 01.04.2021

Süleyman Kargın Osman Doğru İlknur Küçükosmanoğlu

https://doi.org/10.28982/josam.840890

Abstract

Amaç: Midline ve transvers laparotomi arasındaki üstünlükler hala tartışmalıdır. Bu çalışmanın amacı, midline ve transvers kesilerden sonar yara iyileşmesinde önemi olan kollajenler ve total kolajen miktarları açısından fark olup olmadığını ortaya çıkarmaktır.
Yöntemler: Yirmi dört BALB/C faresi rastgele üç gruba (kontrol, midline ve transvers gruplar) ayrıldı. Laparotomilerden 28 gün sonar yapılan cerrahi girişimle doku örnekleri alındı. Örnekler histokimyasal inceleme ile total kolajen yoğunluğu, Tip I ve Tip III kolajen miktarları ve maturasyon indeksi açısından incelendi.
Bulgular: Transvers grupta total kollajen yoğunluğu, Tip I kollajen oranı ve maturasyon indeksi midline grubuna gore biraz daha yüksekti ve Tip III kollajen oranı transvers grupta midline grubuna gore daha düşüktü, ancak bu fark istatistiksel olarak anlamlı değildi (sırasıyla p = 0,486, p = 0,541, p = 0,336, p = 0,541).
Sonuç: Bu deneysel çalışmada total kollajen yoğunluğu ve kollajen maturasyon indeksi dikkate alındığında yara iyileşmesi üzerine transvers laparotomilerin midline laparotomilere üstünlüğünü vurgulayabilir.

Keywords

Laparotomi Kollajen Yara iyileşmesi Maturasyon indeksi

References

  • 1. Hodgson NC, Malthaner RA, Ostbye T. The search for an ideal method of abdominal fascial closure: a meta-analysis. Ann Surg. 2000;231:436-42.
  • 2. Weiland DE, Bay RC, Del Sordi S. Choosing the best abdominal closure by meta-analysis. Am J Surg. 1998;176:666-70.
  • 3. Halm JA, Lip H, Schmitz PI, Jeekel J. Incisional hernia after upper abdominal surgery: a randomised controlled trial of midline versus transverse incision. Hernia. 2009;13:275-80.
  • 4. Grantcharov TP, Rosenberg J. Vertical compared with transverse incisions in abdominal surgery. Eur J Surg. 2001;167:260-7.
  • 5. Brown SR, Goodfellow PB. Transverse verses midline incisions for abdominal surgery. Cochrane Database Syst Rev. 2005;(4):CD005199.
  • 6. Lawson L. All abdominal incisions should be transverse. J Am Coll Surg. 2014;219:846.
  • 7. Seiler CM, Deckert A, Diener MK, Knaebel HP, Weigand MA, Victor N, et al. Midline versus transverse incision in major abdominal surgery: a randomized, double-blind equivalence trial (POVATI: ISRCTN60734227). Ann Surg. 2009;249:913-20.
  • 8. Patel SV, Paskar DD, Nelson RL, Vedula SS, Steele SR. Closure methods for laparotomy incisions for preventing incisional hernias and other wound complications. Cochrane Database Syst Rev. 2017;11:CD005661.
  • 9. Lee L, Mata J, Droeser RA, Kaneva P, Liberman S, Charlebois P, et al. Incisional Hernia After Midline Versus Transverse Specimen Extraction Incision: A Randomized Trial in Patients Undergoing Laparoscopic Colectomy. Ann Surg. 2018;268:41-7.
  • 10. Wang PH, Huang BS, Horng HC, Yeh CC, Chen YJ. Wound healing. J Chin Med Assoc. 2018;81:94-101.
  • 11. Burgeson RE, Nimni ME. Collagen types. Molecular structure and tissue distribution. Clin Orthop Relat Res. 1992;(282):250-72.
  • 12. Höer JJ, Junge K, Schachtrupp A, Klinge U, Schumpelick V. Influence of laparotomy closure technique on collagen synthesis in the incisional region. Hernia. 2002;6:93-8.
  • 13. Bellón JM, Pérez-López P, Simón-Allue R, Sotomayor S, Pérez-Köhler B, Peña E, et al. New suture materials for midline laparotomy closure: an experimental study. BMC Surg. 2014;14:70.
  • 14. National Research Council (US) Institute for Laboratory Animal Research. Guide for the Care and Use of Laboratory Animals. Washington (DC): National Academies Press (US); 1996.
  • 15. Anderson JR. Suture length to wound length ratio and healing of midline laparotomy incisions. Br J Surg. 1994;81:312.
  • 16. Yur M, Çetinkaya Z, Kuloğlu T, Dabak DÖ, Aygen E, Doğru O. The effect of polypropylene mesh on different layers to the formation of collagen type I/III ratio and total collagen density in incisional hernia repair. Int Surg. 2018;5:2942-7.
  • 17. Biondo-Simões MLP, Sichciopi AA, Ioshii SO, Robes RR, Biondo-Simões R. Comparative study of fibrosis induced by Marlex®, Parietex Composite®, Vicryl® and Ultrapro® meshes1. Acta Cir Bras. 2018;33:792-8.
  • 18. Brown SR, Goodfellow PB. Transverse verses midline incisions for abdominal surgery. Cochrane Database Syst Rev. 2005;(4):CD005199.
  • 19. Höer J, Anurov M, Titkova S, Klinge U, Töns C, Ottinger A, et al. Influence of suture material and suture technique on collagen fibril diameters in midline laparotomies. Eur Surg Res. 2000;32:359-67.
  • 20. Nilsson T. The relative rate of wound healing in longitudinal and transverse laparotomy incisions. Animal experiments. Acta Chir Scand. 1982;148:251-6.
  • 21. Chalya PL, Massinde AN, Kihunrwa A, Mabula JB. Abdominal fascia closure following elective midline laparotomy: a surgical experience at a tertiary care hospital in Tanzania. BMC Res Notes. 2015;8:281.
  • 22. Inaba T, Okinaga K, Fukushima R, Iinuma H, Ogihara T, Ogawa F, et al. Prospective randomized study of two laparotomy incisions for gastrectomy: midline incision versus transverse incision. Gastric Cancer. 2004;7:167-71.
  • 23. Lawson L. All abdominal incisions should be transverse. J Am Coll Surg. 2014;219:846.
  • 24. Parkinson J, Brass A, Canova G, Brechet Y. The mechanical properties of simulated collagen fibrils. J Biomech. 1997;30:549-54.
  • 25. Birk DE, Fitch JM, Babiarz JP, Doane KJ, Linsenmayer TF. Collagen fibrillogenesis in vitro: interaction of types I and V collagen regulates fibril diameter. J Cell Sci. 1990;95 ( Pt 4):649-57.
  • 26. Caetano GF, Fronza M, Leite MN, Gomes A, Frade MA. Comparison of collagen content in skin wounds evaluated by biochemical assay and by computer-aided histomorphometric analysis. Pharm Biol. 2016;54:2555-9.
  • 27. Reddy GK, Enwemeka CS. A simplified method for the analysis of hydroxyproline in biological tissues. Clin Biochem. 1996;29:225-9.
  • 28. Ignat’eva NY, Danilov NA, Averkiev SV, ObrezkovaMV, Lunin VV. Determination of hydroxyproline in tissues and the evaluation of the collagen content of the tissues. J Anal Chem. 2007;62:51-7.
  • 29. Kliment CR, Englert JM, Crum LP, Oury TD. A novel method for accurate collagen and biochemical assessment of pulmonary tissue utilizing one animal. Int J Clin Exp Pathol. 2011;4:349-55.

Are the collagen types and density in the wound healed after midline and transverse laparotomy different? An experimental study in mice

Year 2021, Volume: 5 Issue: 4, 331 - 335, 01.04.2021

Süleyman Kargın Osman Doğru İlknur Küçükosmanoğlu

https://doi.org/10.28982/josam.840890

Abstract

Background/Aim: Whether midline or transverse incision is superior in laparotomy is still controversial. The aim of this study was to investigate the total collagen amounts in wound healing after midline and transverse incisions and reveal whether there was a difference between these two laparotomy techniques.
Methods: Twenty-four BALB/c mice were randomly divided into three groups (control, midline, and transverse groups). Mice were sacrificed 28 days after the surgical procedures. The samples were examined histochemically in terms of total collagen density, Type I and Type III collagen rate and maturation index.
Results: Total collagen density, Type I collagen rate and maturation index were slightly insignificantly higher and Type III collagen rate was insignificantly lower in the transverse incision group compared to the midline group (P=0.486, P=0.541, P=0.336 and P=0.541, respectively).
Conclusion: The total collagen density, Tip I and Tip III collagen amounts, and maturation index of wounds healed after transverse and midline laparotomy are similar according to this experimental model.

Keywords

Laparotomy collagen wound healing maturation index

References

  • 1. Hodgson NC, Malthaner RA, Ostbye T. The search for an ideal method of abdominal fascial closure: a meta-analysis. Ann Surg. 2000;231:436-42.
  • 2. Weiland DE, Bay RC, Del Sordi S. Choosing the best abdominal closure by meta-analysis. Am J Surg. 1998;176:666-70.
  • 3. Halm JA, Lip H, Schmitz PI, Jeekel J. Incisional hernia after upper abdominal surgery: a randomised controlled trial of midline versus transverse incision. Hernia. 2009;13:275-80.
  • 4. Grantcharov TP, Rosenberg J. Vertical compared with transverse incisions in abdominal surgery. Eur J Surg. 2001;167:260-7.
  • 5. Brown SR, Goodfellow PB. Transverse verses midline incisions for abdominal surgery. Cochrane Database Syst Rev. 2005;(4):CD005199.
  • 6. Lawson L. All abdominal incisions should be transverse. J Am Coll Surg. 2014;219:846.
  • 7. Seiler CM, Deckert A, Diener MK, Knaebel HP, Weigand MA, Victor N, et al. Midline versus transverse incision in major abdominal surgery: a randomized, double-blind equivalence trial (POVATI: ISRCTN60734227). Ann Surg. 2009;249:913-20.
  • 8. Patel SV, Paskar DD, Nelson RL, Vedula SS, Steele SR. Closure methods for laparotomy incisions for preventing incisional hernias and other wound complications. Cochrane Database Syst Rev. 2017;11:CD005661.
  • 9. Lee L, Mata J, Droeser RA, Kaneva P, Liberman S, Charlebois P, et al. Incisional Hernia After Midline Versus Transverse Specimen Extraction Incision: A Randomized Trial in Patients Undergoing Laparoscopic Colectomy. Ann Surg. 2018;268:41-7.
  • 10. Wang PH, Huang BS, Horng HC, Yeh CC, Chen YJ. Wound healing. J Chin Med Assoc. 2018;81:94-101.
  • 11. Burgeson RE, Nimni ME. Collagen types. Molecular structure and tissue distribution. Clin Orthop Relat Res. 1992;(282):250-72.
  • 12. Höer JJ, Junge K, Schachtrupp A, Klinge U, Schumpelick V. Influence of laparotomy closure technique on collagen synthesis in the incisional region. Hernia. 2002;6:93-8.
  • 13. Bellón JM, Pérez-López P, Simón-Allue R, Sotomayor S, Pérez-Köhler B, Peña E, et al. New suture materials for midline laparotomy closure: an experimental study. BMC Surg. 2014;14:70.
  • 14. National Research Council (US) Institute for Laboratory Animal Research. Guide for the Care and Use of Laboratory Animals. Washington (DC): National Academies Press (US); 1996.
  • 15. Anderson JR. Suture length to wound length ratio and healing of midline laparotomy incisions. Br J Surg. 1994;81:312.
  • 16. Yur M, Çetinkaya Z, Kuloğlu T, Dabak DÖ, Aygen E, Doğru O. The effect of polypropylene mesh on different layers to the formation of collagen type I/III ratio and total collagen density in incisional hernia repair. Int Surg. 2018;5:2942-7.
  • 17. Biondo-Simões MLP, Sichciopi AA, Ioshii SO, Robes RR, Biondo-Simões R. Comparative study of fibrosis induced by Marlex®, Parietex Composite®, Vicryl® and Ultrapro® meshes1. Acta Cir Bras. 2018;33:792-8.
  • 18. Brown SR, Goodfellow PB. Transverse verses midline incisions for abdominal surgery. Cochrane Database Syst Rev. 2005;(4):CD005199.
  • 19. Höer J, Anurov M, Titkova S, Klinge U, Töns C, Ottinger A, et al. Influence of suture material and suture technique on collagen fibril diameters in midline laparotomies. Eur Surg Res. 2000;32:359-67.
  • 20. Nilsson T. The relative rate of wound healing in longitudinal and transverse laparotomy incisions. Animal experiments. Acta Chir Scand. 1982;148:251-6.
  • 21. Chalya PL, Massinde AN, Kihunrwa A, Mabula JB. Abdominal fascia closure following elective midline laparotomy: a surgical experience at a tertiary care hospital in Tanzania. BMC Res Notes. 2015;8:281.
  • 22. Inaba T, Okinaga K, Fukushima R, Iinuma H, Ogihara T, Ogawa F, et al. Prospective randomized study of two laparotomy incisions for gastrectomy: midline incision versus transverse incision. Gastric Cancer. 2004;7:167-71.
  • 23. Lawson L. All abdominal incisions should be transverse. J Am Coll Surg. 2014;219:846.
  • 24. Parkinson J, Brass A, Canova G, Brechet Y. The mechanical properties of simulated collagen fibrils. J Biomech. 1997;30:549-54.
  • 25. Birk DE, Fitch JM, Babiarz JP, Doane KJ, Linsenmayer TF. Collagen fibrillogenesis in vitro: interaction of types I and V collagen regulates fibril diameter. J Cell Sci. 1990;95 ( Pt 4):649-57.
  • 26. Caetano GF, Fronza M, Leite MN, Gomes A, Frade MA. Comparison of collagen content in skin wounds evaluated by biochemical assay and by computer-aided histomorphometric analysis. Pharm Biol. 2016;54:2555-9.
  • 27. Reddy GK, Enwemeka CS. A simplified method for the analysis of hydroxyproline in biological tissues. Clin Biochem. 1996;29:225-9.
  • 28. Ignat’eva NY, Danilov NA, Averkiev SV, ObrezkovaMV, Lunin VV. Determination of hydroxyproline in tissues and the evaluation of the collagen content of the tissues. J Anal Chem. 2007;62:51-7.
  • 29. Kliment CR, Englert JM, Crum LP, Oury TD. A novel method for accurate collagen and biochemical assessment of pulmonary tissue utilizing one animal. Int J Clin Exp Pathol. 2011;4:349-55.
There are 29 citations in total.

Details

Primary Language English
Subjects Surgery
Journal Section Research article
Authors

Süleyman Kargın 0000-0003-4597-8654

Osman Doğru 0000-0002-8761-3904

İlknur Küçükosmanoğlu 0000-0002-5181-6152

Publication Date April 1, 2021
Published in Issue Year 2021 Volume: 5 Issue: 4

Cite

APA Kargın, S., Doğru, O., & Küçükosmanoğlu, İ. (2021). Are the collagen types and density in the wound healed after midline and transverse laparotomy different? An experimental study in mice. Journal of Surgery and Medicine, 5(4), 331-335. https://doi.org/10.28982/josam.840890
AMA Kargın S, Doğru O, Küçükosmanoğlu İ. Are the collagen types and density in the wound healed after midline and transverse laparotomy different? An experimental study in mice. J Surg Med. April 2021;5(4):331-335. doi:10.28982/josam.840890
Chicago Kargın, Süleyman, Osman Doğru, and İlknur Küçükosmanoğlu. “Are the Collagen Types and Density in the Wound Healed After Midline and Transverse Laparotomy Different? An Experimental Study in Mice”. Journal of Surgery and Medicine 5, no. 4 (April 2021): 331-35. https://doi.org/10.28982/josam.840890.
EndNote Kargın S, Doğru O, Küçükosmanoğlu İ (April 1, 2021) Are the collagen types and density in the wound healed after midline and transverse laparotomy different? An experimental study in mice. Journal of Surgery and Medicine 5 4 331–335.
IEEE S. Kargın, O. Doğru, and İ. Küçükosmanoğlu, “Are the collagen types and density in the wound healed after midline and transverse laparotomy different? An experimental study in mice”, J Surg Med, vol. 5, no. 4, pp. 331–335, 2021, doi: 10.28982/josam.840890.
ISNAD Kargın, Süleyman et al. “Are the Collagen Types and Density in the Wound Healed After Midline and Transverse Laparotomy Different? An Experimental Study in Mice”. Journal of Surgery and Medicine 5/4 (April 2021), 331-335. https://doi.org/10.28982/josam.840890.
JAMA Kargın S, Doğru O, Küçükosmanoğlu İ. Are the collagen types and density in the wound healed after midline and transverse laparotomy different? An experimental study in mice. J Surg Med. 2021;5:331–335.
MLA Kargın, Süleyman et al. “Are the Collagen Types and Density in the Wound Healed After Midline and Transverse Laparotomy Different? An Experimental Study in Mice”. Journal of Surgery and Medicine, vol. 5, no. 4, 2021, pp. 331-5, doi:10.28982/josam.840890.
Vancouver Kargın S, Doğru O, Küçükosmanoğlu İ. Are the collagen types and density in the wound healed after midline and transverse laparotomy different? An experimental study in mice. J Surg Med. 2021;5(4):331-5.
 Download Cover Image