Anti-Staphylococcal Activity of Verbascum thapsus L. against Methicillin-Resistant Staphylococcus aureus
Abstract
Present study was conducted to investigate
the anti-Staphylococcal activity
of ethanol extract obtained from the leaves of Verbascum thapsus L. (Scrophulariaceae) against
Methicillin-resistant Staphylococcus
aureus (MRSA) by agar–well diffusion method and microdilution method. The
ethanol extract showed antibacterial activity against Methicillin-resistant Staphylococcus aureus (MRSA) strains (MIC <1024 µg/mL). When compared with
Methicillin and Gentamicin, the extract was more effective against MRSA
strains. It is therefore suggested that the extract obtained from V. thapsus could be used as an anti-Staphylococcal
agent.
Keywords
Verbascum thapsus L. Agar-well diffusion method microdilution method Methicillin-resistant Staphylococcus aureus
References
- 1. Millspaugh CF. American medicinal plants. Dover Publishing Inc., New York, 1974; p. 430-434.
- 2. Tyler VE, Herbs of choice: the therapeutic use of phytomedicinals. Pharmaceutical Products Press, New York, 1994; p. 92.
- 3. Turker AU, Camper ND. Biological activity of common mullein, a medicinal plant. Journal of Ethnopharmacology, 2002; 82:117-125.
- 4. Khan NH, nur-E Kamal M.S.A, Rahman M. Antibacterial activity of Euphorbia thymifolia Linn. Indian Journal of Medical Research, 1988; 87:395-397.
- 5. Javadpour MM, Juban MM, Lo WC, et al. De novo antimicrobial peptides with low mammalian cell toxicity. Journal of Medicinal Chemistry, 1996; 39:3107-3113.
- 6. Collins CM, Lyne PM. Microbiological Methods. Butterworths and Co. Ltd., London, 1987; p. 316.
- 7. Zgoda JR, Porter JR. A convenient microdilution method for screening natural products against bacteria and fungi. Pharmaceutical Biology, 2001; 39:221-225.
- 8. Clinical and Laboratory Standards Institute (CLSI). Methods for dilution antimicrobial susceptibility tests bacteria that grow aerobically, 3rd edition, approved standard. NCCLS document M100-S12, Wayne, PA, USA. 2002.
- 9. Jonathan SG, Fasidi IO. Antimicrobial activities of two Nigerian edible macrofungi, Lycoperdon pusilum and L. giganteum. African Journal of Biomedical Research, 2003; 6: 85-90.
- 10. Hartleb I, Seifert K. Sangarosaponin-D a triterpenoid saponin from Verbacsum songaricum. Phytochemistry, 1994; 35:1009-1011.
- 11. Klimek B. Hidroxycinnamoyl ester glycosides and saponins from flowers of Verbascum phlomoides. Phytochemistry, 1996; 43:1281-1284.
- 12. Skaltsounis AL, Tsitsa-Tzardis E, Demetzos C, et al. Unduloside, a new iridoid glycoside from Verbascum undulatum. Journal of Naturel Product, 1996; 59:673-675.
- 13. Youhnovski N, Dandarov K, Guggisberg A, et al. Macrocyclic spermine alcholoids from Verbascum: Isolation, structure elucidation and syntheses of the (E/Z)- isomeric pairs(S)-verbasikrine/ (S)-isoverbasikrine and (S)-verbamekrine /(S)-isoverbamekrine. Helvetica Chimica Acta, 1999; 82:1185-1194.
- 14. Elgindi MR, Mabry TJ. Phenyletanoid glycosides from Verbascum siniaticum. Asian Journal of Chemistry, 2000; 12:127-130.
- 15. Seifert KH, Jahne S, Hesse M. Verbascenine, ein macrocyclische spermin alkoloid aus Verbascum. Helvetica Chimica Acta, 1982; 65:2540-2547.
- 16. Koblikova Z, Turecek F, Ninova P, et al. Verbaskine, a macrocyclic spermin alcholoide of a novel type from Verbascum pseudonobile Stoj. et Stef. (Scrophulariaceae). Tetrahedron Letters, 1983; 24:4381-4384.
- 17. Pascual Teresa DJ, Diaz F, Grande M. Componentes del Varbascum thapsus L. I. Triterpenos. An Quím, 1978; 74:311-314.
- 18. Pascual Teresa DJ, Diaz F, Grande M. Componentes del Varbascum thapsus L. II. Aceite de las semillas. An Quím, 1978b; 78C:107-110.
- 19. Pascual Teresa DJ, Diaz F, Grande M. Componentes del Varbascum thapsus L. III. Contribucion al estudio de las saponinas. An Quím 1980; 76:107-110.
- 20. Cowan M.M. Plant products as antimicrobial agents. Clinical Microbiology Reviews, 1999; 12:564-582.
- 21. Mehrotra R, Ahmed B, Vishwakarma RA, et al. Verbacoside. A new luteolin glycoside from Verbascum thapsus. Journal of Naturel Product, 1989; N52: 640-643.
- 22. McCutcheon AR, Ellis SM, Hancock REW, et al. Antibiotic screening of medicinal plants of the British Columbian native peoples. Journal of Ethnopharmacology, 1992; 37:213-223.
- 23. McCutcheon AR, Ellis SM, Hancock R.E.W, et al. Antifungal screening of medicinal plants of the British Columbian native peoples. Journal of Ethnopharmacology, 1994; 44:157-169.
- 24. McCutcheon, AR, Roberts TE, Gibbons E, et al. Antiviral screening of British Columbian medicinal plants. Journal of Ethnopharmacology, 1995; 49:101-110.
- 25. Gross KL, Werner PA. The Biology of Canadian weeds: Verbascum thapsus and Verbascum blattaria. Canadian Journal Plant Science, 1978; 58:401-403.
- 26. Elakkia SA, Venkatesalu V. Anti-MRSA activity of different extracts of selected Cassia species. International Journal of Pharmacy and Clinical Sciences, 2014; 4:11-17.
- 27. Lee HW, Yoon J.H, Sohn JH, et al. Detection of MecA gene in clinical isolates of Staphylococcus aureus by multiplex-PCR, and antimicrobial susceptibility of MRSA. Journal of Microbiology and Biotechnology, 2003; 13:354-359.
Methicillin-Dirençli Staphylococcus aureus Bakterilerine Karşı Verbascum thapsus L. Bitkisinin Anti-Staphylococcal Aktivitesi
Abstract
Bu çalışmada, Verbascum thapsus L. (Scrophulariaceae)
bitkisinin yapraklarından elde edilen etanol ekstraktının anti-Staphylococcal
aktivitesi Methicillin-Dirençli Staphylococcus aureus (MRSA) bakterilerine
karşı Agar-kuyu difüzyon metodu ve mikrodilüsyon metodu ile araştırılmıştır.
Etanol ekstraktı Methicillin-Dirençli Staphylococcus
aureus (MRSA) bakterilerine karşı (MIC
<1024 µg/mL) antibakteriyal bir aktivite göstermiştir. Methicillin ve
Gentamicin antibiyotikleri ile karşılaştırıldığında, ekstraktın MRSA strainlerine(suşlarına)
karşı oldukça etkili olduğu saptanmıştır. Bu nedenle bitkinin bir anti-Staphylococcal
ajan olarak kullanılabilirliği önerilmektedir. (Bu nedenle bitkinin bir
anti-Staphylococcal ajan olarak kullanılabilirliği in vitro olarak uygun
bulunmuş ve in vivo çalışmaların
yapılması gerektiği düşünülmüştür)
Keywords
Verbascum thapsus L. Agar-kuyu difüzyon metodu Mikrodilüsyon metodu Methicillin-Dirençli Staphylococcus aureus
References
- 1. Millspaugh CF. American medicinal plants. Dover Publishing Inc., New York, 1974; p. 430-434.
- 2. Tyler VE, Herbs of choice: the therapeutic use of phytomedicinals. Pharmaceutical Products Press, New York, 1994; p. 92.
- 3. Turker AU, Camper ND. Biological activity of common mullein, a medicinal plant. Journal of Ethnopharmacology, 2002; 82:117-125.
- 4. Khan NH, nur-E Kamal M.S.A, Rahman M. Antibacterial activity of Euphorbia thymifolia Linn. Indian Journal of Medical Research, 1988; 87:395-397.
- 5. Javadpour MM, Juban MM, Lo WC, et al. De novo antimicrobial peptides with low mammalian cell toxicity. Journal of Medicinal Chemistry, 1996; 39:3107-3113.
- 6. Collins CM, Lyne PM. Microbiological Methods. Butterworths and Co. Ltd., London, 1987; p. 316.
- 7. Zgoda JR, Porter JR. A convenient microdilution method for screening natural products against bacteria and fungi. Pharmaceutical Biology, 2001; 39:221-225.
- 8. Clinical and Laboratory Standards Institute (CLSI). Methods for dilution antimicrobial susceptibility tests bacteria that grow aerobically, 3rd edition, approved standard. NCCLS document M100-S12, Wayne, PA, USA. 2002.
- 9. Jonathan SG, Fasidi IO. Antimicrobial activities of two Nigerian edible macrofungi, Lycoperdon pusilum and L. giganteum. African Journal of Biomedical Research, 2003; 6: 85-90.
- 10. Hartleb I, Seifert K. Sangarosaponin-D a triterpenoid saponin from Verbacsum songaricum. Phytochemistry, 1994; 35:1009-1011.
- 11. Klimek B. Hidroxycinnamoyl ester glycosides and saponins from flowers of Verbascum phlomoides. Phytochemistry, 1996; 43:1281-1284.
- 12. Skaltsounis AL, Tsitsa-Tzardis E, Demetzos C, et al. Unduloside, a new iridoid glycoside from Verbascum undulatum. Journal of Naturel Product, 1996; 59:673-675.
- 13. Youhnovski N, Dandarov K, Guggisberg A, et al. Macrocyclic spermine alcholoids from Verbascum: Isolation, structure elucidation and syntheses of the (E/Z)- isomeric pairs(S)-verbasikrine/ (S)-isoverbasikrine and (S)-verbamekrine /(S)-isoverbamekrine. Helvetica Chimica Acta, 1999; 82:1185-1194.
- 14. Elgindi MR, Mabry TJ. Phenyletanoid glycosides from Verbascum siniaticum. Asian Journal of Chemistry, 2000; 12:127-130.
- 15. Seifert KH, Jahne S, Hesse M. Verbascenine, ein macrocyclische spermin alkoloid aus Verbascum. Helvetica Chimica Acta, 1982; 65:2540-2547.
- 16. Koblikova Z, Turecek F, Ninova P, et al. Verbaskine, a macrocyclic spermin alcholoide of a novel type from Verbascum pseudonobile Stoj. et Stef. (Scrophulariaceae). Tetrahedron Letters, 1983; 24:4381-4384.
- 17. Pascual Teresa DJ, Diaz F, Grande M. Componentes del Varbascum thapsus L. I. Triterpenos. An Quím, 1978; 74:311-314.
- 18. Pascual Teresa DJ, Diaz F, Grande M. Componentes del Varbascum thapsus L. II. Aceite de las semillas. An Quím, 1978b; 78C:107-110.
- 19. Pascual Teresa DJ, Diaz F, Grande M. Componentes del Varbascum thapsus L. III. Contribucion al estudio de las saponinas. An Quím 1980; 76:107-110.
- 20. Cowan M.M. Plant products as antimicrobial agents. Clinical Microbiology Reviews, 1999; 12:564-582.
- 21. Mehrotra R, Ahmed B, Vishwakarma RA, et al. Verbacoside. A new luteolin glycoside from Verbascum thapsus. Journal of Naturel Product, 1989; N52: 640-643.
- 22. McCutcheon AR, Ellis SM, Hancock REW, et al. Antibiotic screening of medicinal plants of the British Columbian native peoples. Journal of Ethnopharmacology, 1992; 37:213-223.
- 23. McCutcheon AR, Ellis SM, Hancock R.E.W, et al. Antifungal screening of medicinal plants of the British Columbian native peoples. Journal of Ethnopharmacology, 1994; 44:157-169.
- 24. McCutcheon, AR, Roberts TE, Gibbons E, et al. Antiviral screening of British Columbian medicinal plants. Journal of Ethnopharmacology, 1995; 49:101-110.
- 25. Gross KL, Werner PA. The Biology of Canadian weeds: Verbascum thapsus and Verbascum blattaria. Canadian Journal Plant Science, 1978; 58:401-403.
- 26. Elakkia SA, Venkatesalu V. Anti-MRSA activity of different extracts of selected Cassia species. International Journal of Pharmacy and Clinical Sciences, 2014; 4:11-17.
- 27. Lee HW, Yoon J.H, Sohn JH, et al. Detection of MecA gene in clinical isolates of Staphylococcus aureus by multiplex-PCR, and antimicrobial susceptibility of MRSA. Journal of Microbiology and Biotechnology, 2003; 13:354-359.
Details
| Subjects | Health Care Administration |
|---|---|
| Journal Section | Articles |
| Authors | |
| Publication Date | March 23, 2017 |
| Published in Issue | Year 2017 Volume: 9 Issue: 1 |
