Research Article
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Determination of Some Important Viruses in Cowpea (Vigna unguiculata L.) Seeds from Isparta and Antalya Provinces

Year 2021, Volume: 12 Issue: Ek (Suppl.) 1, 486 - 490, 31.12.2021
https://doi.org/10.29048/makufebed.884867

Abstract

This study was carried out to detect the presence of seed borne viruses in cowpea seeds collected from Isparta and Antalya provinces during 2016-2017. The seed samples were tested by Double Sandwich Enzyme Linked Immunosorbent Assay (DAS-ELISA) for Bean common mosaic virus (BCMV) and Cowpea mild mottle virus (CpMMV). Based on the results, the seed samples were infected with BCMV and CpMMV. Infection ratio for BCMV and CpMMV was determined as 22.22% and 11.11% respectively. The mixed infection of both viruses with a rate of 2.22% was detected in only two of the samples tested. Our knowledge CpMMV was firstly reported at cowpea plants in Turkey.

Project Number

2209A

References

  • Abdullahi, I., Ikotun, T., Winter, S., Thottappilly, G., Atiri, G.I. (2001). Investigation on seed transmission of Cucumber mosaic virus in cowpea. African Crop Science Journal 9(4): 677-684.
  • Bariana, H.S., Shannon, A.L., Chu, P.W.G., Waterhouse, P.M. (1994). Detection of five seedborne legume viruses in one sensitive multiplex polymerase chain reaction test. Phytopathology 84: 1201-1205.
  • Çulal-Kılıç, H., Yardımcı, N., Açıkyürek, S., Uzal, A. (2015). Detection of BCMV, AMV and CMV by DAS-ELISA and Immunocapture-RT-PCR in bean growing areas in The West Mediterranean Region, Turkey. Fresenius Environmental Bulletin 24(5): 1752-1756.
  • Çulal-Kılıç, H., Özdoğan, H.M., Vural, M.C. (2020). Isparta ili börülce tohumlarında Soybean Mosaic Virus. Fen ve matematik bilimlerinde akademik çalışmalar. III. Bölüm, Livre De Lyon Basımevi, Fransa, 53-60.
  • Fidan, U., Yorgancı, U. (1990). Investigation on the detection and seed transmission of the virus diseases occuring on pulse crops in Aegean Region. Journal of Turkish Phytopathology 19(1): 1-5.
  • Gülümser, A. (2016). Dünyada ve Türkiye’de yemeklik dane baklagillerin durumu. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi, 25 (Özel sayı-1): 292-298.
  • Habib, S. A., El-Atta, O.K., El-Hammady, M., Awad, M. (1981). Interaction between Bean common mosaic virus and Bean yellow mosaic virus in relation to morphological characters of bean plants. Ain Shams University, Faculty of Agriculture Research Bulletin 1606: 1-14.
  • Hao, N.B., Albrechtsen, S.E., Nicolaisen, M. (2003). Detection and identification of the blackeye cowpea mosaic strain of Bean common mosaic virus in seeds of Vigna unguiculata spp. from North Vietnam. Australasian Plant Pathology 32: 505-509.
  • Huang, X., Bie, Z. (2010). Cinnamic acid-inhibited ribulose-1.5-bisphosphate carboxylase activitiy is mediated through decrease spermine and changes in the ratio of polamines in cowpea. Journal of Plant Physiology 167: 47-53.
  • Hughes, J.A., Shoyinka, S.A. (2003). Overview of viruses of legumes other than groundnut in Africa,” in Plant Virology in Sub-Sahara Africa, Hughes, J.A. and Odu J. (eds.), Proceedings of a conference organized by IITA Ibadan, Nigeria, International Institute of Tropical Agriculture, 553–568.
  • Kısa, D. (2010). Tuz stresinin börülce (Vigna unguiculata L. ) yağ asiti içeriğine etkisinin araştırılması. Gaziosman paşa Üniversitesi, Fen Bilimleri Enstitüsü, Biyoloji Anabilim Dalı. Yüksek Lisans Tezi, 69s.
  • Mali, V.R., Thottappilly, G. (1986). Virus diseases of cowpea in the tropics. In Review of tropical plant diseases, Raychsudhri, S.P. Verma, J.P. (eds.), Today and Tomorrow Publishers, New Delhi, India.
  • Ojuederie, O.B., Odu, B.O., Ilori, C.O. (2009). Serological detection of seed borne viruses in cowpea regenerated germplasm using protein A sandwich enzyme linked immunorsorbent assay. African Crop Science Journal, 17(3): 125 – 132.
  • Omar, R.A., Mehiar, F.F., Zayed, E.A., Deit, A.A. (1985). Biological studies onsome seed-borne viruses and their effect on vegetative growth and yield component of the host plant. Acta Phytopathologica Acadiae Scientiarum Hungaricae, 20(3): 80-85.
  • Paylan, İ.C., Erkan, S. (2013.) Bazı Sebze tohumlarındaki viral etmenlerin saptanması ve yaygınlık oranlarının belirlenmesi. Ege Üniversitesi, Ziraat Fakültesi Dergisi, 50(3): 231-240.
  • Salem, N.M., Ehlers, J.D., Roberts, P.A., Ng, J.C.K. (2010). Biological and molecular diagnosis of seedborne viruses in cowpea germplasm of geographically diverse sub-Saharan Origins. Plant Pathology 59: 773-784.
  • Şehirali, S. (1988). Yemeklik Dane Baklagiller, Ankara Üniversitesi Ziraat Fakültesi Yayınları, Ankara.
  • Tavasoli, M., Shahraeen, N., Ghorbani, Sh., Hashemi, S.H. (2007). Identification of cowpea mild mottle virus-CPMMV in soybean from Iran. Proceedings of the 4th Iranian Virology Congress: Medical University of Tehran, Iran, 342.
  • TUIK (2018). Bitkisel Üretim İstatistikleri. http://www.tuik.gov.tr (Erişim Tarihi: 22.05.2018).
  • Yılmaz, M.A., Baloğlu, S., Özaslan, M., Güldür, M.E. (1995). GAP bölgesinde kültür bitkilerinde belirlenen virüsler. GAP Bölgesi Bitki Koruma Sorunları ve Çözüm Önerileri Sempozyumu: 27-29 Nisan, 1995, Şanlıurfa, Türkiye, Kongre Kitabı, 241- 250.
  • Yılmaz M.A., Özaslan, D. (1989). Determination of cowpea aphid borne mosaic virus by enzyme linked immunosorbent assay on cowpea and bean seeds. Doğa, Türk Tarım ve Ormancılık Dergisi, 13(3a): 870-873.

Isparta ve Antalya İllerindeki Börülce (Vigna unguiculata L.) Tohumlarında Bazı Önemli Virüs Hastalıklarının Belirlenmesi

Year 2021, Volume: 12 Issue: Ek (Suppl.) 1, 486 - 490, 31.12.2021
https://doi.org/10.29048/makufebed.884867

Abstract

Bu çalışma Isparta ve Antalya illerinden toplanan börülce tohum örneklerinde Fasulye adi mozayik virüsü (Bean common mosaic virus: BCMV) ve Börülce hafif benek virüsü (Cowpea mild mottle virus: CpMMV)’nün belirlenmesi amacı ile 2016-2017 yıllarında yürütülmüştür. Tohum örnekleri BCMV ve CpMMV için Double Sandwich Enzyme Linked Immunosorbent Assay (DAS-ELISA) yöntemi ile analiz edilmiştir. Elde edilen sonuçlara göre tohum örneklerinin BCMV ve CpMMV ile enfekteli olduğu belirlenmiş ve börülce tohum örneklerinde BCMV ve CpMMV etmenlerinin bulunma oranlarının sırasıyla %22.22 ve %11.11 olduğu saptanmıştır. Örneklerin sadece 2 adetinde %2.22 oranında her iki virüsün karışık enfeksiyonu tespit edilmiştir. Bu çalışma ile Türkiye’de CpMMV etmeni börülcede serolojik olarak ilk kez belirlenmiştir.

Supporting Institution

TUBİTAK

Project Number

2209A

Thanks

Bu çalışma ‘TÜBİTAK 2209 A-Üniversite Öğrencileri Yurt İçi / Yurt Dışı Araştırma Projeleri Destekleme Programı’ kapsamında desteklenmiştir. Maddi olanak sağlayan TÜBİTAK’a teşekkür ederiz.

References

  • Abdullahi, I., Ikotun, T., Winter, S., Thottappilly, G., Atiri, G.I. (2001). Investigation on seed transmission of Cucumber mosaic virus in cowpea. African Crop Science Journal 9(4): 677-684.
  • Bariana, H.S., Shannon, A.L., Chu, P.W.G., Waterhouse, P.M. (1994). Detection of five seedborne legume viruses in one sensitive multiplex polymerase chain reaction test. Phytopathology 84: 1201-1205.
  • Çulal-Kılıç, H., Yardımcı, N., Açıkyürek, S., Uzal, A. (2015). Detection of BCMV, AMV and CMV by DAS-ELISA and Immunocapture-RT-PCR in bean growing areas in The West Mediterranean Region, Turkey. Fresenius Environmental Bulletin 24(5): 1752-1756.
  • Çulal-Kılıç, H., Özdoğan, H.M., Vural, M.C. (2020). Isparta ili börülce tohumlarında Soybean Mosaic Virus. Fen ve matematik bilimlerinde akademik çalışmalar. III. Bölüm, Livre De Lyon Basımevi, Fransa, 53-60.
  • Fidan, U., Yorgancı, U. (1990). Investigation on the detection and seed transmission of the virus diseases occuring on pulse crops in Aegean Region. Journal of Turkish Phytopathology 19(1): 1-5.
  • Gülümser, A. (2016). Dünyada ve Türkiye’de yemeklik dane baklagillerin durumu. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi, 25 (Özel sayı-1): 292-298.
  • Habib, S. A., El-Atta, O.K., El-Hammady, M., Awad, M. (1981). Interaction between Bean common mosaic virus and Bean yellow mosaic virus in relation to morphological characters of bean plants. Ain Shams University, Faculty of Agriculture Research Bulletin 1606: 1-14.
  • Hao, N.B., Albrechtsen, S.E., Nicolaisen, M. (2003). Detection and identification of the blackeye cowpea mosaic strain of Bean common mosaic virus in seeds of Vigna unguiculata spp. from North Vietnam. Australasian Plant Pathology 32: 505-509.
  • Huang, X., Bie, Z. (2010). Cinnamic acid-inhibited ribulose-1.5-bisphosphate carboxylase activitiy is mediated through decrease spermine and changes in the ratio of polamines in cowpea. Journal of Plant Physiology 167: 47-53.
  • Hughes, J.A., Shoyinka, S.A. (2003). Overview of viruses of legumes other than groundnut in Africa,” in Plant Virology in Sub-Sahara Africa, Hughes, J.A. and Odu J. (eds.), Proceedings of a conference organized by IITA Ibadan, Nigeria, International Institute of Tropical Agriculture, 553–568.
  • Kısa, D. (2010). Tuz stresinin börülce (Vigna unguiculata L. ) yağ asiti içeriğine etkisinin araştırılması. Gaziosman paşa Üniversitesi, Fen Bilimleri Enstitüsü, Biyoloji Anabilim Dalı. Yüksek Lisans Tezi, 69s.
  • Mali, V.R., Thottappilly, G. (1986). Virus diseases of cowpea in the tropics. In Review of tropical plant diseases, Raychsudhri, S.P. Verma, J.P. (eds.), Today and Tomorrow Publishers, New Delhi, India.
  • Ojuederie, O.B., Odu, B.O., Ilori, C.O. (2009). Serological detection of seed borne viruses in cowpea regenerated germplasm using protein A sandwich enzyme linked immunorsorbent assay. African Crop Science Journal, 17(3): 125 – 132.
  • Omar, R.A., Mehiar, F.F., Zayed, E.A., Deit, A.A. (1985). Biological studies onsome seed-borne viruses and their effect on vegetative growth and yield component of the host plant. Acta Phytopathologica Acadiae Scientiarum Hungaricae, 20(3): 80-85.
  • Paylan, İ.C., Erkan, S. (2013.) Bazı Sebze tohumlarındaki viral etmenlerin saptanması ve yaygınlık oranlarının belirlenmesi. Ege Üniversitesi, Ziraat Fakültesi Dergisi, 50(3): 231-240.
  • Salem, N.M., Ehlers, J.D., Roberts, P.A., Ng, J.C.K. (2010). Biological and molecular diagnosis of seedborne viruses in cowpea germplasm of geographically diverse sub-Saharan Origins. Plant Pathology 59: 773-784.
  • Şehirali, S. (1988). Yemeklik Dane Baklagiller, Ankara Üniversitesi Ziraat Fakültesi Yayınları, Ankara.
  • Tavasoli, M., Shahraeen, N., Ghorbani, Sh., Hashemi, S.H. (2007). Identification of cowpea mild mottle virus-CPMMV in soybean from Iran. Proceedings of the 4th Iranian Virology Congress: Medical University of Tehran, Iran, 342.
  • TUIK (2018). Bitkisel Üretim İstatistikleri. http://www.tuik.gov.tr (Erişim Tarihi: 22.05.2018).
  • Yılmaz, M.A., Baloğlu, S., Özaslan, M., Güldür, M.E. (1995). GAP bölgesinde kültür bitkilerinde belirlenen virüsler. GAP Bölgesi Bitki Koruma Sorunları ve Çözüm Önerileri Sempozyumu: 27-29 Nisan, 1995, Şanlıurfa, Türkiye, Kongre Kitabı, 241- 250.
  • Yılmaz M.A., Özaslan, D. (1989). Determination of cowpea aphid borne mosaic virus by enzyme linked immunosorbent assay on cowpea and bean seeds. Doğa, Türk Tarım ve Ormancılık Dergisi, 13(3a): 870-873.
There are 21 citations in total.

Details

Primary Language Turkish
Subjects Agricultural, Veterinary and Food Sciences
Journal Section Research Paper
Authors

Handan Çulal Kılıç 0000-0003-4020-9442

Leyla Doğutaş This is me 0000-0003-0039-6319

Ebru Yılan This is me 0000-0002-4142-4032

Project Number 2209A
Publication Date December 31, 2021
Acceptance Date December 7, 2021
Published in Issue Year 2021 Volume: 12 Issue: Ek (Suppl.) 1

Cite

APA Çulal Kılıç, H., Doğutaş, L., & Yılan, E. (2021). Isparta ve Antalya İllerindeki Börülce (Vigna unguiculata L.) Tohumlarında Bazı Önemli Virüs Hastalıklarının Belirlenmesi. Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 12(Ek (Suppl.) 1), 486-490. https://doi.org/10.29048/makufebed.884867