Objectives
The use of liquid chromatography with tandem mass spectrometry (LC-MS/MS) device has increased significantly in toxicology validation laboratories in recent years. The maintenance cost of this expensive equipment is high as well as the cost of consumables. Reduction of the matrix effect and preparation more clear samples are very important for the validation of methods in clinical laboratories.
The primary goal is to create a cleaner urine sample preparation technique to reduce the cost of maintenance of the LC-MS/MS device without affecting test results.
Methods
We prepared the patients’ urine in two different ways; routine urine preparation method and used our centrifuged method (14000 rpm, 10 minutes) for routine illicit substance use. The standard material used to determine whether there was a statistical difference in the urine sample with both different methods was added to both urine samples.
Results
Our findings showed that there was no statistical difference between the results of both methods for detection of illicit substance use. There was no difference between the high and low quantities of the 14 illicit substances measured and the centrifuged method and routine urine preparation methods (p<0.05). However, the urine sample obtained by our newly developed centrifuged method was cleaner, lucid and homogeneous.
Conclusion
Preliminary studies show that although the new centrifuged method is time-consuming, it can be reliable. The long-term use of this centrifugal method can reduce the costs of device maintenance, repair and consumption. So initial findings suggest that column costs and replacement processes may be positively affected.
Primary Language | English |
---|---|
Subjects | Biochemistry and Cell Biology (Other) |
Journal Section | Research Articles |
Authors | |
Publication Date | September 16, 2022 |
Submission Date | November 9, 2020 |
Published in Issue | Year 2022 Volume: 3 Issue: 2 |
The content published in NTMS is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.