Which one to choose for anti-cytokeratin immunohistochemistry in small gastric biopsies: Bleached Periodic acid schiff- Alcian blue (PAB) or May Grunwald Giemsa (MGG) sections?
Abstract
Endoscopic biopsies are commonly used for diagnosis in gastrointestinal tractus diseases. The dimensions of the biopsy samples taken during endoscopic examination may sometimes not allow additional examinations or the tissues may become smaller during routine technical procedures. In gastric endoscopic biopsies, hematoxylin&eosin (H&E) staining, Periodic acid schiff- Alcian blue (PAB) histochemistry for intestinal metaplasia, and giemsa histochemistry for Helicobacter pylori are used routinely in many in laboratories. In cases where tumor is suspected, immunohistochemical staining is performed in addition to these stainings. The most commonly performed staining is anti-cytokeratin.
For various reasons, additional staining applied to materials with reduced tissue size may not yield results. In this case, the patient may need to undergo an endoscopic examination again for definitive diagnosis. To avoid or at least minimize this situation, this study is planned to evaluate the diagnostic usability of restaining with anti-cytokeratin on bleached PAB and MGG sections that were performed for routine examination.
Keywords
Anti-cytokeratin Bleaching Restaining Periodic acid schiff- Alcian blue May Grunwald Giemsa
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References
- 1. Wissowzky A. Ueber das Eosin als reagenz auf Hämoglobin und die Bildung von Blutgefässen und Blutkörperchen bei Säugetier und Hühnerembryonen. Arch Mikrosk Anat 1876;13:479-496.
- 2. Riva MA, Manzoni M, Isimbaldi G, Cesana G, Pagni F. Histochemistry: historical development and current use in pathology. Biotech Histochem. 2014 Feb;89(2):81-90.
- 3. Donald Van Hecke. Routine Immunohistochemical Staining Today: Choices to Make, Challenges to Take. Journal of Histotechnology 25:1,45-54.
- 4. Marrack J. Nature of antibodies. Nature. 1934;133:292–3
- 5. Bodey B. The significance of immunohistochemistry in the diagnosis and therapy of neoplasms. Expert Opin Biol Ther. 2002 Apr;2(4):371-93.
- 6. Jaffer S, Bleiweiss IJ. Beyond hematoxylin and eosin--the role of immunohistochemistry in surgical pathology. Cancer Invest. 2004;22(3):445-65.
- 7. Roskams T. The role of immunohistochemistry in diagnosis. Clin Liver Dis. 2002 May;6(2):571-89, x.
- 8. Leong AS, Wright J. The contribution of immunohistochemical staining in tumour diagnosis. Histopathology. 1987;11:1295–305.
- 9. Taylor CR, Levenson RM. Quantification of immunohistochemistry—issues concerning methods, utility and semiquantitative assessment II. Histopathology. 2006 Oct;49(4):411-24.
- 10. Dardik M, Epstein JI. Efficacy of restaining prostate needle biopsies with high-molecular weight cytokeratin. Hum Pathol. 2000 Sep;31(9):1155-61.
- 11. Cavusoglu T, Kılıc KD, Buhur A, Adali Y, Yigitturk G, Erbas O, Uyanikgil Y. Immunohistochemical restaining of hematoxylin and eosin stained slides: a histotechnical study. FNG& Bilim Dergisi 2016;2(4):260-5.
- 12. Hinton JP, Dvorak K, Roberts E, French WJ, Grubbs JC, Cress AE, et al. A Method to Reuse Archived H&E Stained Histology Slides for a Multiplex Protein Biomarker Analysis. Methods Protoc. 2019 Nov 15;2(4):86.
- 13. Ozawa A, Sakaue M. New decolorization method produces more information from tissue sections stained with hematoxylin and eosin stain and masson-trichrome stain. Ann Anat. 2020 Jan;227:151431.
Abstract
Keywords
Anti-cytokeratin Bleaching Restaining Periodic acid schiff- Alcian blue May Grunwald Giemsa
Project Number
-
References
- 1. Wissowzky A. Ueber das Eosin als reagenz auf Hämoglobin und die Bildung von Blutgefässen und Blutkörperchen bei Säugetier und Hühnerembryonen. Arch Mikrosk Anat 1876;13:479-496.
- 2. Riva MA, Manzoni M, Isimbaldi G, Cesana G, Pagni F. Histochemistry: historical development and current use in pathology. Biotech Histochem. 2014 Feb;89(2):81-90.
- 3. Donald Van Hecke. Routine Immunohistochemical Staining Today: Choices to Make, Challenges to Take. Journal of Histotechnology 25:1,45-54.
- 4. Marrack J. Nature of antibodies. Nature. 1934;133:292–3
- 5. Bodey B. The significance of immunohistochemistry in the diagnosis and therapy of neoplasms. Expert Opin Biol Ther. 2002 Apr;2(4):371-93.
- 6. Jaffer S, Bleiweiss IJ. Beyond hematoxylin and eosin--the role of immunohistochemistry in surgical pathology. Cancer Invest. 2004;22(3):445-65.
- 7. Roskams T. The role of immunohistochemistry in diagnosis. Clin Liver Dis. 2002 May;6(2):571-89, x.
- 8. Leong AS, Wright J. The contribution of immunohistochemical staining in tumour diagnosis. Histopathology. 1987;11:1295–305.
- 9. Taylor CR, Levenson RM. Quantification of immunohistochemistry—issues concerning methods, utility and semiquantitative assessment II. Histopathology. 2006 Oct;49(4):411-24.
- 10. Dardik M, Epstein JI. Efficacy of restaining prostate needle biopsies with high-molecular weight cytokeratin. Hum Pathol. 2000 Sep;31(9):1155-61.
- 11. Cavusoglu T, Kılıc KD, Buhur A, Adali Y, Yigitturk G, Erbas O, Uyanikgil Y. Immunohistochemical restaining of hematoxylin and eosin stained slides: a histotechnical study. FNG& Bilim Dergisi 2016;2(4):260-5.
- 12. Hinton JP, Dvorak K, Roberts E, French WJ, Grubbs JC, Cress AE, et al. A Method to Reuse Archived H&E Stained Histology Slides for a Multiplex Protein Biomarker Analysis. Methods Protoc. 2019 Nov 15;2(4):86.
- 13. Ozawa A, Sakaue M. New decolorization method produces more information from tissue sections stained with hematoxylin and eosin stain and masson-trichrome stain. Ann Anat. 2020 Jan;227:151431.
Details
| Primary Language | English |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Research Article |
| Authors | |
| Project Number | - |
| Early Pub Date | August 1, 2023 |
| Publication Date | July 19, 2023 |
| Submission Date | December 20, 2022 |
| Acceptance Date | February 9, 2023 |
| Published in Issue | Year 2023 Volume: 40 Issue: 2 |
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