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The Effects of Selenıum on The Blood Oxıdatıve Stress Parametres in The Perıapıcal Lesıon Whıch Was Formed on Rats

Year 2021, Volume: 12 Issue: 3, 309 - 318, 25.12.2021

Nergiz Bolat Bulem Ureyen Kaya İlter İlhan Halil Aşçı Yalçın Erzurumlu

https://doi.org/10.22312/sdusbed.954377

Abstract

Objective: To investigate the effects of selenium against blood serum oxidative stress and inflammation markers after periapical lesion induction.

Material and Method: In this study, 30 adult male Sprague-Dawley rats were randomly divided into negative control, positive control and selenium groups (n=10). Periapical lesion was induced in mandibular first molars in the positive control and selenium groups. Rats in selenium group were applied to intraperitoneal selenium during 28 days. The blood taken on the day 28 was homogenized and was used in the analysis of the IL-6, total oxidant capacity (TOS), total antioxidant capacity (TAS), oxidative stress index (OSI), ischemia modified albumin (IMA), native thiol (NT) and total thiol (TT) parameters.

Results: Statistically significant difference was determined among the groups in terms of serum IL-6 level (p<0.01). The lowest serum IL-6 level was detected in the negative control group (p<0.01). Statistically significant differences were observed among the groups in terms of TAS, TOS and OSI levels. While the highest serum TAS level was in the negative control group, there was no statistical difference between the selenium and negative control groups. The highest TT and NT values among the groups were detected in the selenium group (p<0.01), and there was no statistical difference between the negative and positive control groups in terms of NT value (p=0.052). No statistically significant difference was found in terms of IMA and Disulfide values among groups (p>0.05).

Conclusions: Selenium, which is known to increase antioxidant enzyme synthesis, is thought to be effective in maintaining systemic health by playing a protective role against the increase in oxidant and inflammatory parameters in the blood as a result of local chronic infections. 

Keywords

Periapical Lesion Periapical lesion oxidative stress selenium total antioxidant status total oxidant status

Supporting Institution

BAP

Project Number

8040

References

  • [1] Garlet GP (2010) Destructive and protective roles of cytokines in periodontitis: a re-appraisal from host defense and tissue destruction viewpoints. J Dent Res 89:1349–1363.
  • [2] Reiner S.L.: Development in motion: helper T cells at work. Cell 2007; 129: pp. 33-36.
  • [3] Martinho F.C., Chiesa W.M., Leite F.R., et. al.: Correlation between clinical/radiographic features and inflammatory cytokine networks produced by macrophages stimulated with endodontic content. J Endod 2012; 38: pp. 740-745.
  • [4] Araujo-Pires AC, Francisconi CF, Biguetti CC, et al. Simultaneous analysis of T helper subsets (Th1, Th2, Th9, Th17, Th22, Tfh, Tr1 and Tregs) markers expression in periapical lesions reveals multiple cytokine clusters accountable for lesions activity and inactivity status. Journal of Applied Oral Science 2014; 22, 336– 46.
  • [5] D'Aiuto F., Parkar M., Andreou G., et. al.: Periodontitis and systemic inflammation: control of the local infection is associated with a reduction in serum inflammatory markers. J Dent Res 2004; 83: pp. 156-160.
  • [6] Wang J, Du Y, Deng J, Wang X, Long F, He J. MicroRNA-506 Is Involved in Regulation of the Occurrence of Lipopolysaccharides (LPS)-Induced Pulpitis by Sirtuin 1 (SIRT1). Med Sci Monit. 2019 Dec 26; 25: 10008-10015.
  • [7] Inchingolo F, Marrelli M, Annibali S, et al. Influence of endodontic treatment on systemic oxidative stress. Int J Med Sci. 2014; 11: 1–69.
  • [8] Vengerfeldt V, Mändar R, Saag M, Piir A, Kullisaar T. Oxidative stress in patients with endodontic pathologies. J Pain Res. 2017;1 0: 2031-2040.
  • [9] Miller W.D.: The human mouth as a focus of infection. Dent Cosmos 1891; 33: pp. 689-713.
  • [10] Darling B.C.: Roentgen-ray indications for tooth extraction the medical roentgenologist offers an impartial survey for the physician, the dentist, and the patient. J Dent Res 1919; 1: pp. 391-412.
  • [11] Newman H.N.: Focal infection. J Dent Res 1996; 75: pp. 1912-1919.
  • [12] Cintra L.T., da Silva Facundo A.C., Prieto A.K., et. al.: Blood profile and histology in oral infections associated with diabetes. J Endod 2014; 40: pp. 1139-1144.
  • [13] Samuel RO, Gomes-Filho JE, Azuma MM, Sumida DH, Oliveira SH, Chiba FY et al. Endodontic infections increase leukocyte and lymphocyte levels in the blood. Clin Oral Investig. 2018 Apr;22 (3): 1395-401.
  • [14] Arababadi M.K., Pourfathollah A.A., Jafarzadeh A., Hassanshahi G. Serum levels of IL-10 and IL-17A in occult HBV-infected south-east Iranian patients. Hepat Mon. 2010; 10 (1): 31–35.
  • [15] Azuma Mm, Gomes-Fılho Je, Ervolıno E, Cardoso Cbm,Pıpa Cb, Kawaı T, Contı Lc, Cıntra Lta. Omega-3 Fattyacids Reduce İnflammation İn Rat Apical Periodontitis. J Endod 2018; 44: 604–608.34.
  • [16] Aksoy U, Savtekin G, Şehirli AÖ, Kermeoğlu F, Kalender A, Özkayalar H, Sayıner S, Orhan K. Effects of alpha-lipoic acid therapy on experimentally induced apical periodontitis: a biochemical, histopathological and micro-CT analysis. Int Endod J. 2019 Sep; 52(9): 1317-1326.
  • [17] Gomes C, Martinho FC, Barbosa DS et al. Increased root canal endotoxin levels are associated with chronic apical periodontitis, increased oxidative and nitrosative stress, major depression, severity of depression, and a lowered quality of life. Molecular Neurobiology 2018; 55, 2814–27.
  • [18] Patel MD, Shakir QJ, Shetty A. Interrelationship between chronic periodontitis and anemia: a 6-month follow-up study. J Indian SocPeriodontol. 2014; 18(1): 19-25.
  • [19] Thomas B, Ramesh A, Suresh S, Prasad BR. A comparative evaluation of antioxidant enzymes and selenium in the serum of periodontitis patients with diabetes mellitus type 2. Contemp Clin Dent 2013; 4:176–180.
  • [20] Atli M, Erikoglu M, Kaynak A, Esen HH, Kurban S. The effects of selenium and vitamin E on lung tissue in rats with sepsis. Clin Invest Med. 2012; 35(2): 48-54.
  • [21] Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem 2005; 38, 1103-1111.
  • [22] Bar-Or D, Lau E, Winkler JV. A novel assay for cobalt-albumin binding and its potential as a marker for miyocardial ischemia-a preliminary report. J Emerg Med 2000; 19: 311-315.
  • [23] Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clinical biochem. 2014; 47(18): 326–32.
  • [24] Arya S, Duhan J, Tewari S, Sangwan P, Ghalaut V, Aggarwal S. Healing of apical periodontitis after nonsurgical treatment in patients with type 2 diabetes. J Endod. 2017 Oct; 43(10): 1623-7.
  • [25] Pereira RF, Cintra LT, Tessarin GW, Chiba FY, Mattera MSLC, Scaramele NF, et al. Periapical lesions increase macrophage infiltration and inflammatory signaling in muscle tissue of rats. J Endod. 2017 Jun;43(6):982-8.
  • [26] Zhang J, Huang X, Lu B, Zhang C, Cai Z. Can apical periodontitis affect serum levels of CRP, IL-2, and IL-6 as well as induce pathological changes in remote organs? Clin Oral Investig. 2016 Sep; 20(7): 1617-24.
  • [27] Bashashati M., Moradi M., Sarosiek I.: Interleukin-6 in irritable bowel syndrome: a systematic review and meta-analysis of IL-6 (-G174C) and circulating IL-6 levels. Cytokine 2017; 99: pp. 132-138.
  • [28] Simpson R.J., Hammacher A., Smith D.K., et. al.: Interleukin-6: structure-function relationships. Protein Sci 1997; 6: pp. 929-955.
  • [29] Graunaite I, Lodiene G, Maciulskiene V. Pathogenesis of apical periodontitis: a literature review. J Oral Maxillofac Res. 2011; 2: e1.
  • [30] Sarıtekin E, Üreyen Kaya B, Aşcı H, Özmen Ö. Anti-inflammatory and antiresorptive functions of melatonin on experimentally induced periapical lesions. Int Endod J. 2019 Oct; 52(10): 1466-1478.
  • [31] Higuchi, A.; Inoue, H.; Kawakita, T.; Ogishima, T.; Tsubota, K. Selenium Compound Protects Corneal Epithelium against Oxidative Stress. PLoS ONE 2012; 7, e45612.
  • [32] Lippi G, Montagnana M, Salvagno GL, Guidi GC. Potential value for new diagnostic markers in the early recognition of acute coronary syndromes. CJEM 2006; 8: 27–31.
  • [33] Chen W, Zhao Y, Seefeldt T, Guan X. Determination of thiols and disulfides via HPLC quantification of 5-thio-2-nitrobenzoic acid. Journal of Pharmaceutical and Biomedical Analysis 2008; 48: 1375-1380.
  • [34] Brulisauer L, Gauthier MA, Leroux JC. Disulfide-containing parenteral delivery systems and their redox-biological fate. Journal of Controlled Release 2014; 195: 147-154.
  • [35] Sanchez-Rodriguez MA, Mendoza-Nunez VM. Oxidative stress indexes for diagnosis of health or disease in humans. Oxidative Medicine and Cellular Longevity 2019; 2019: 4128152.

Deney Hayvanlarında Oluşturulan Periapikal Lezyon Modelinde Selenyumun Kandaki Oksidatif Stres Parametreleri Üzerine Etkileri

Year 2021, Volume: 12 Issue: 3, 309 - 318, 25.12.2021

Nergiz Bolat Bulem Ureyen Kaya İlter İlhan Halil Aşçı Yalçın Erzurumlu

https://doi.org/10.22312/sdusbed.954377

Abstract

Amaç: Bu çalışmanın amacı selenyumun ratlarda deneysel olarak indüklenen periapikal lezyon varlığında kan inflamasyon ve oksidatif stres belirteçleri üzerine etkilerinin araştırılmasıdır.

Gereç ve Yöntem: Bu çalışmada 30 adet erişkin Sprague-Dawley cinsi erkek rat rastgele negatif kontrol, pozitif kontrol ve selenyum grubu olmak üzere 3 gruba ayrıldı. Pozitif kontrol ve selenyum grubundaki ratlarda alt çene 1. azı dişlerinde periapikal lezyon oluşumu tetiklendi. Selenyum grubundaki ratlara deney süresince (28 gün) periton içine selenyum uygulandı. Yirmi sekinci günde alınan kan örneklerinde IL-6, total oksidan (TOS), total antioksidan (TAS) kapasite, oksidatif stres indeksi (OSİ), iskemi modifiye albümin (İMA), natif tiyol (NT) ve total tiyol (TT) seviyeleri değerlendirildi.

Bulgular: Gruplar arasında serum IL-6 seviyesi açısından istatistiksel olarak anlamlı fark tespit edildi (p<0,01). Selenyum grubundaki IL-6 seviyesi pozitif kontrol grubundan daha düşüktü (p<0,01). Gruplar arasında TAS, TOS ve OSİ seviyeleri açısından istatistiksel olarak anlamlı farklılık gözlendi (p<0,01). Gruplar arasında en yüksek serum TAS seviyesi negatif kontrol grubunda tespit edilirken, selenyum grubu ile aralarında istatistiksel bir fark bulunmadı. Gruplar arasında en yüksek OSİ değeri pozitif kontrol grubunda tespit edildi (p<0,05). OSİ değeri selenyum grubunda pozitif kontrol grubuna göre anlamlı derecede düşüktü (p<0,01). Gruplar arasında en yüksek TT ve NT değerleri selenyum grubunda tespit edildi (p<0,01). Gruplar arası karşılaştırmalarda İMA ve Disülfid değerleri bakımından istatistiksel olarak anlamlı bir fark saptanmadı (p>0,05).

Sonuç: Antioksidan enzim sentezini artırdığı bilinen selenyumun periapikal lezyon gibi lokal kronik enfeksiyonlar sonucu kanda meydana gelen oksidan ve enflamatuvar parametrelerin artışına karşı koruyucu rol oynayarak sistemik sağlığın devam ettirilmesinde etkili olacağı düşünülmektedir. 

Keywords

Periapikal lezyon oksidatif stres selenyum total oksidan kapasite total antioksidan kapasite

Project Number

8040

References

  • [1] Garlet GP (2010) Destructive and protective roles of cytokines in periodontitis: a re-appraisal from host defense and tissue destruction viewpoints. J Dent Res 89:1349–1363.
  • [2] Reiner S.L.: Development in motion: helper T cells at work. Cell 2007; 129: pp. 33-36.
  • [3] Martinho F.C., Chiesa W.M., Leite F.R., et. al.: Correlation between clinical/radiographic features and inflammatory cytokine networks produced by macrophages stimulated with endodontic content. J Endod 2012; 38: pp. 740-745.
  • [4] Araujo-Pires AC, Francisconi CF, Biguetti CC, et al. Simultaneous analysis of T helper subsets (Th1, Th2, Th9, Th17, Th22, Tfh, Tr1 and Tregs) markers expression in periapical lesions reveals multiple cytokine clusters accountable for lesions activity and inactivity status. Journal of Applied Oral Science 2014; 22, 336– 46.
  • [5] D'Aiuto F., Parkar M., Andreou G., et. al.: Periodontitis and systemic inflammation: control of the local infection is associated with a reduction in serum inflammatory markers. J Dent Res 2004; 83: pp. 156-160.
  • [6] Wang J, Du Y, Deng J, Wang X, Long F, He J. MicroRNA-506 Is Involved in Regulation of the Occurrence of Lipopolysaccharides (LPS)-Induced Pulpitis by Sirtuin 1 (SIRT1). Med Sci Monit. 2019 Dec 26; 25: 10008-10015.
  • [7] Inchingolo F, Marrelli M, Annibali S, et al. Influence of endodontic treatment on systemic oxidative stress. Int J Med Sci. 2014; 11: 1–69.
  • [8] Vengerfeldt V, Mändar R, Saag M, Piir A, Kullisaar T. Oxidative stress in patients with endodontic pathologies. J Pain Res. 2017;1 0: 2031-2040.
  • [9] Miller W.D.: The human mouth as a focus of infection. Dent Cosmos 1891; 33: pp. 689-713.
  • [10] Darling B.C.: Roentgen-ray indications for tooth extraction the medical roentgenologist offers an impartial survey for the physician, the dentist, and the patient. J Dent Res 1919; 1: pp. 391-412.
  • [11] Newman H.N.: Focal infection. J Dent Res 1996; 75: pp. 1912-1919.
  • [12] Cintra L.T., da Silva Facundo A.C., Prieto A.K., et. al.: Blood profile and histology in oral infections associated with diabetes. J Endod 2014; 40: pp. 1139-1144.
  • [13] Samuel RO, Gomes-Filho JE, Azuma MM, Sumida DH, Oliveira SH, Chiba FY et al. Endodontic infections increase leukocyte and lymphocyte levels in the blood. Clin Oral Investig. 2018 Apr;22 (3): 1395-401.
  • [14] Arababadi M.K., Pourfathollah A.A., Jafarzadeh A., Hassanshahi G. Serum levels of IL-10 and IL-17A in occult HBV-infected south-east Iranian patients. Hepat Mon. 2010; 10 (1): 31–35.
  • [15] Azuma Mm, Gomes-Fılho Je, Ervolıno E, Cardoso Cbm,Pıpa Cb, Kawaı T, Contı Lc, Cıntra Lta. Omega-3 Fattyacids Reduce İnflammation İn Rat Apical Periodontitis. J Endod 2018; 44: 604–608.34.
  • [16] Aksoy U, Savtekin G, Şehirli AÖ, Kermeoğlu F, Kalender A, Özkayalar H, Sayıner S, Orhan K. Effects of alpha-lipoic acid therapy on experimentally induced apical periodontitis: a biochemical, histopathological and micro-CT analysis. Int Endod J. 2019 Sep; 52(9): 1317-1326.
  • [17] Gomes C, Martinho FC, Barbosa DS et al. Increased root canal endotoxin levels are associated with chronic apical periodontitis, increased oxidative and nitrosative stress, major depression, severity of depression, and a lowered quality of life. Molecular Neurobiology 2018; 55, 2814–27.
  • [18] Patel MD, Shakir QJ, Shetty A. Interrelationship between chronic periodontitis and anemia: a 6-month follow-up study. J Indian SocPeriodontol. 2014; 18(1): 19-25.
  • [19] Thomas B, Ramesh A, Suresh S, Prasad BR. A comparative evaluation of antioxidant enzymes and selenium in the serum of periodontitis patients with diabetes mellitus type 2. Contemp Clin Dent 2013; 4:176–180.
  • [20] Atli M, Erikoglu M, Kaynak A, Esen HH, Kurban S. The effects of selenium and vitamin E on lung tissue in rats with sepsis. Clin Invest Med. 2012; 35(2): 48-54.
  • [21] Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem 2005; 38, 1103-1111.
  • [22] Bar-Or D, Lau E, Winkler JV. A novel assay for cobalt-albumin binding and its potential as a marker for miyocardial ischemia-a preliminary report. J Emerg Med 2000; 19: 311-315.
  • [23] Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clinical biochem. 2014; 47(18): 326–32.
  • [24] Arya S, Duhan J, Tewari S, Sangwan P, Ghalaut V, Aggarwal S. Healing of apical periodontitis after nonsurgical treatment in patients with type 2 diabetes. J Endod. 2017 Oct; 43(10): 1623-7.
  • [25] Pereira RF, Cintra LT, Tessarin GW, Chiba FY, Mattera MSLC, Scaramele NF, et al. Periapical lesions increase macrophage infiltration and inflammatory signaling in muscle tissue of rats. J Endod. 2017 Jun;43(6):982-8.
  • [26] Zhang J, Huang X, Lu B, Zhang C, Cai Z. Can apical periodontitis affect serum levels of CRP, IL-2, and IL-6 as well as induce pathological changes in remote organs? Clin Oral Investig. 2016 Sep; 20(7): 1617-24.
  • [27] Bashashati M., Moradi M., Sarosiek I.: Interleukin-6 in irritable bowel syndrome: a systematic review and meta-analysis of IL-6 (-G174C) and circulating IL-6 levels. Cytokine 2017; 99: pp. 132-138.
  • [28] Simpson R.J., Hammacher A., Smith D.K., et. al.: Interleukin-6: structure-function relationships. Protein Sci 1997; 6: pp. 929-955.
  • [29] Graunaite I, Lodiene G, Maciulskiene V. Pathogenesis of apical periodontitis: a literature review. J Oral Maxillofac Res. 2011; 2: e1.
  • [30] Sarıtekin E, Üreyen Kaya B, Aşcı H, Özmen Ö. Anti-inflammatory and antiresorptive functions of melatonin on experimentally induced periapical lesions. Int Endod J. 2019 Oct; 52(10): 1466-1478.
  • [31] Higuchi, A.; Inoue, H.; Kawakita, T.; Ogishima, T.; Tsubota, K. Selenium Compound Protects Corneal Epithelium against Oxidative Stress. PLoS ONE 2012; 7, e45612.
  • [32] Lippi G, Montagnana M, Salvagno GL, Guidi GC. Potential value for new diagnostic markers in the early recognition of acute coronary syndromes. CJEM 2006; 8: 27–31.
  • [33] Chen W, Zhao Y, Seefeldt T, Guan X. Determination of thiols and disulfides via HPLC quantification of 5-thio-2-nitrobenzoic acid. Journal of Pharmaceutical and Biomedical Analysis 2008; 48: 1375-1380.
  • [34] Brulisauer L, Gauthier MA, Leroux JC. Disulfide-containing parenteral delivery systems and their redox-biological fate. Journal of Controlled Release 2014; 195: 147-154.
  • [35] Sanchez-Rodriguez MA, Mendoza-Nunez VM. Oxidative stress indexes for diagnosis of health or disease in humans. Oxidative Medicine and Cellular Longevity 2019; 2019: 4128152.
There are 35 citations in total.

Details

Primary Language Turkish
Subjects Health Care Administration
Journal Section Araştırma Articlesi
Authors

Nergiz Bolat 0000-0003-0469-6466

Bulem Ureyen Kaya 0000-0002-8295-4347

İlter İlhan 0000-0003-3739-9580

Halil Aşçı 0000-0002-1545-035X

Yalçın Erzurumlu 0000-0001-6835-4436

Project Number 8040
Publication Date December 25, 2021
Submission Date June 18, 2021
Published in Issue Year 2021 Volume: 12 Issue: 3

Cite

Vancouver Bolat N, Ureyen Kaya B, İlhan İ, Aşçı H, Erzurumlu Y. Deney Hayvanlarında Oluşturulan Periapikal Lezyon Modelinde Selenyumun Kandaki Oksidatif Stres Parametreleri Üzerine Etkileri. Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi. 2021;12(3):309-18.
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