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Gundelia tournefortii L. Bitki Ekstraktlarının Antimikrobiyal Aktivitelerinin ve İn Vitro Sitotoksik Aktivitelerinin Değerlendirilmesi

Year 2019, Volume: 26 Issue: 4, 436 - 442, 01.12.2019
https://doi.org/10.17343/sdutfd.534049

Abstract

Giriş: Kenger, Asteraceae familyasından Gundelia tournefortii bitkisi, tıbbi bir bitkidir. Kenger'in çiçeklerinin, yapraklarının, tohumlarının ve köklerinin besin kaynağı olarak kullanıldığı bilinmektedir.

Materyal ve method:Bu çalışmada Sivas ilinde, taze filizleri soyularak çiğ ve pişirilerek yenilen Kenger’in gövde kısımlarının su fazı ve uçucu yağ ekstraksiyonu yapılarak, özütlerin antimikrobiyal ve in vitro antiproliferatif özellikleri bakımından değerlendirilmesi amaçlanmıştır. Sitotoksik aktiviteleri Meme Kanseri Hücre Hattı (MCF-7) ve insan endotelyal hücre hattı (HUVEC) kullanılarak araştırılmıştır. Bitki özütlerinin hücre kültürlerindeki etkileri, XTT yöntemiyle Eliza reader cihazında absorbans bulunarak elde edilmiştir. Kenger bitki özütlerinin MCF-7 hücreleri ve endotelyal hücreler üzerinde sitotoksik etkileri belirlenmiştir. 

Bulgular:Kenger bitki özütlerinin zayıf antimikrobiyal etkilerinin olduğu MIC değerleriyle ortaya konulmuştur.  

Sonuç:Sivas ilinde halk arasında şifalı olduğu bilinen, bahar ve yaz aylarında büyüyen bitki kısımları kesilip soyularak iç kısımlarının besin olarak tüketildiği, çeşitli hastalıkların tedavisinde kullanılan Kenger bitkisinin, denediğimiz hücreler üzerinde sitotoksik etkilerinin olduğu, zayıf antimikrobiyal etkisinin bulunduğu görülmektedir.   

References

  • 1. Çoban ÖE, Patır B. Use of some spices and herbs antioxidant affected in foods. Electronic Journal of Food Technologies 2010; 5(2): 7-19.
  • 2. Önde S, Vurdu H. Bitki çeşitliliği ve unutulan gen kaynakları. Tabiat ve İnsan 1988; 22(2): 27-31.
  • 3. Yücel E, Güney F, Şengün İY. The wild plants consumed as a food in Mihalıççık district (Eskişehir/Turkey) and consumption forms of these plants. Biological Diversity and Conservation 2010; 3(3): 158-175.
  • 4. Yapıcı Ü, Hoşgören H, Saya Ö. Ethnobotanic features of Kurtalan (Siirt) province. Dicle University Journal of Ziya Gökalp Faculty of Education 2009; 12: 191-196.
  • 5. Kırbağ S, Zengin F. Antimicrobial activies of some medical plants in Elazığ. Journal of Agricultural Science 2006; 16(2): 77-80.
  • 6. Demir H. Chemical composition of some wild (Polygonum cgnatum, Tragopoxgom reticulatus and Berberis vulgaris) plants collected from Erzurum. Bahçe 2006; 35(1-2): 55-60.
  • 7. Kaya İ, İncekara N, Nemli Y. Ingredients of some weeds consumed as foof in aegean region. Journal of Agricultural Science 2004; 14(1): 1-6.
  • 8. Yücel E, Tülükoğlu A. Gediz (Kütahya) çevresinde halk ilacı olarak kullanılan bitkiler. Ekoloji (Çevre Dergisi) 2000; 9(36): 12-14.
  • 9. Ertuğ F. An ethnobotanical study in central Anatolia (Turkey). Economic Botany 2000; 54(2): 155-182.
  • 10. Kunkel G. Plants for Human Consumption. 1th ed. Königstein: Koeltz Scientific Books; 1984.
  • 11. Sezik E, Yeşilada E, Honda G, Takaishi Y, Takeda Y, Tanaka T. Traditional medicine in Turkey. Folk medicine in central Anatolia. Journal of Ethnopharmacology 2001; 75(2): 95-115.
  • 12. Coruh N, Celep A S, Özgökçe F, İşcan M. Antioxidant capacities of Gundelia tournefortii L. extracts and inhibition on glutathione-S-transferase activity. Food Chemistry 2007; 100(3): 1249-1253.
  • 13. Tawaha K, Alali F Q, Gharaibeh M, Mohammad M, El-Elimat T. Antioxidant activity and total phenolic content of selected Jordanian plant species. Food Chemistry 2007; 104(4): 1372-1378.
  • 14. Jamshidzadeh A, Fereidooni F, Salehi Z, Niknahad H. Hepatoprotective activity of Gundelia tourenfortii. Journal of Ethnopharmacology 2005; 101(1): 233-237.
  • 15. Haghi G, Hatami A, Arshi R. Distribution of caffeic acid derivatives in Gundelia tournefortii L. Food Chemistry 2011; 124(3): 1029-1035.
  • 16. Wagner H, Nickl H, Aynehchi Y. Molluscicidal saponins from Gundelia tournefortii. Phytochemistry 1984; 23(11): 2505-2508. 17. Sekeroglu N, Senol F S, Orhan I E, Gulpinar A R, Kartal M, Sener B. In vitro prospective effects of various traditional herbal coffees consumed in Anatolia linked to neurodegeneration. Food Research International 2012; 45(1): 197-203.
  • 18. Eloff JN. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Med 1998; 64: 711–713.
  • 19. Franklin R, Matthew A, Alder J, Micheal N, George M, Ferraro MJ et al. Clinical and Laboratory Standards Institute, Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically, Approved Standard (9th ed.), CLSI document M07-A9. Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087, USA, 2012.
  • 20. Pfaller MA, Chaturvedi V, Espinel-Ingroff A, Ghannoum MA, Gosey LL, Odds FC et al. The National Committee for Clinical Laboratory Standards. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts, Approved Standard, (2nd ed)., NCCLS document M27- A2. CLSI, 940 West Valley Road, Suite 1400, Wayne, Pennsylvania 19087- 1898, USA, 2002.
  • 21. Kuete V. Potential of Cameroonian plants and derived products against microbial infections: a review. Planta Med.2010; 76: 1479–1491.
  • 22. Awouafack MD, McGaw LJ, Gottfried S, Mbouangouere R, Tane P, Spiteller M. and Eloff JN. Antimicrobial activity and cytotoxicity of the ethanol extract, fractions and eight compounds isolated from Eriosema robustum (Faba-ceae). BMC Complementary and Alternative Medicine 2013; 13: 289.

Evaluation of Antimicrobial Activities and In Vitro Cytotoxic Activities of Gundelia tournefortii L. Plant Extracts

Year 2019, Volume: 26 Issue: 4, 436 - 442, 01.12.2019
https://doi.org/10.17343/sdutfd.534049

Abstract

Introduction:Kenger, Gundelia tournefortiiplant from Asteraceae family, is a medicinal plant. Flowers, leaves, seeds and roots of Kenger have been reported to be used as a source of food. 

Material ve methods:In this study, it was aimed to evaluate the antimicrobial and in vitroantiproliferative properties of Kenger which fresh sprouts are peeled and eated cooked or uncooked in Sivas. The water phase and essential oil extraction of the body parts of Kenger were aimed to be evaluated in terms of their antimicrobial and in vitroantiproliferative properties.

Results:Cytotoxic activities were investigated using the Breast Cancer Cell Line (MCF-7) and human endothelial cell line (HUVEC). The effects of plant extracts on cell cultures were determined by the absorbance of Eliza reader by XTT method. Kenger plant extracts have been shown to have weak antimicrobial effects with MIC values. 

Conclusions:In the province of Sivas, growing parts of the plant in the spring and summer are cut and peeled into the inner parts of the nutrients. Kenger, which is used in the treatment of various diseases, has cytotoxic effects on the cells we tested and has a weak antimicrobial effect.

References

  • 1. Çoban ÖE, Patır B. Use of some spices and herbs antioxidant affected in foods. Electronic Journal of Food Technologies 2010; 5(2): 7-19.
  • 2. Önde S, Vurdu H. Bitki çeşitliliği ve unutulan gen kaynakları. Tabiat ve İnsan 1988; 22(2): 27-31.
  • 3. Yücel E, Güney F, Şengün İY. The wild plants consumed as a food in Mihalıççık district (Eskişehir/Turkey) and consumption forms of these plants. Biological Diversity and Conservation 2010; 3(3): 158-175.
  • 4. Yapıcı Ü, Hoşgören H, Saya Ö. Ethnobotanic features of Kurtalan (Siirt) province. Dicle University Journal of Ziya Gökalp Faculty of Education 2009; 12: 191-196.
  • 5. Kırbağ S, Zengin F. Antimicrobial activies of some medical plants in Elazığ. Journal of Agricultural Science 2006; 16(2): 77-80.
  • 6. Demir H. Chemical composition of some wild (Polygonum cgnatum, Tragopoxgom reticulatus and Berberis vulgaris) plants collected from Erzurum. Bahçe 2006; 35(1-2): 55-60.
  • 7. Kaya İ, İncekara N, Nemli Y. Ingredients of some weeds consumed as foof in aegean region. Journal of Agricultural Science 2004; 14(1): 1-6.
  • 8. Yücel E, Tülükoğlu A. Gediz (Kütahya) çevresinde halk ilacı olarak kullanılan bitkiler. Ekoloji (Çevre Dergisi) 2000; 9(36): 12-14.
  • 9. Ertuğ F. An ethnobotanical study in central Anatolia (Turkey). Economic Botany 2000; 54(2): 155-182.
  • 10. Kunkel G. Plants for Human Consumption. 1th ed. Königstein: Koeltz Scientific Books; 1984.
  • 11. Sezik E, Yeşilada E, Honda G, Takaishi Y, Takeda Y, Tanaka T. Traditional medicine in Turkey. Folk medicine in central Anatolia. Journal of Ethnopharmacology 2001; 75(2): 95-115.
  • 12. Coruh N, Celep A S, Özgökçe F, İşcan M. Antioxidant capacities of Gundelia tournefortii L. extracts and inhibition on glutathione-S-transferase activity. Food Chemistry 2007; 100(3): 1249-1253.
  • 13. Tawaha K, Alali F Q, Gharaibeh M, Mohammad M, El-Elimat T. Antioxidant activity and total phenolic content of selected Jordanian plant species. Food Chemistry 2007; 104(4): 1372-1378.
  • 14. Jamshidzadeh A, Fereidooni F, Salehi Z, Niknahad H. Hepatoprotective activity of Gundelia tourenfortii. Journal of Ethnopharmacology 2005; 101(1): 233-237.
  • 15. Haghi G, Hatami A, Arshi R. Distribution of caffeic acid derivatives in Gundelia tournefortii L. Food Chemistry 2011; 124(3): 1029-1035.
  • 16. Wagner H, Nickl H, Aynehchi Y. Molluscicidal saponins from Gundelia tournefortii. Phytochemistry 1984; 23(11): 2505-2508. 17. Sekeroglu N, Senol F S, Orhan I E, Gulpinar A R, Kartal M, Sener B. In vitro prospective effects of various traditional herbal coffees consumed in Anatolia linked to neurodegeneration. Food Research International 2012; 45(1): 197-203.
  • 18. Eloff JN. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Med 1998; 64: 711–713.
  • 19. Franklin R, Matthew A, Alder J, Micheal N, George M, Ferraro MJ et al. Clinical and Laboratory Standards Institute, Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically, Approved Standard (9th ed.), CLSI document M07-A9. Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087, USA, 2012.
  • 20. Pfaller MA, Chaturvedi V, Espinel-Ingroff A, Ghannoum MA, Gosey LL, Odds FC et al. The National Committee for Clinical Laboratory Standards. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts, Approved Standard, (2nd ed)., NCCLS document M27- A2. CLSI, 940 West Valley Road, Suite 1400, Wayne, Pennsylvania 19087- 1898, USA, 2002.
  • 21. Kuete V. Potential of Cameroonian plants and derived products against microbial infections: a review. Planta Med.2010; 76: 1479–1491.
  • 22. Awouafack MD, McGaw LJ, Gottfried S, Mbouangouere R, Tane P, Spiteller M. and Eloff JN. Antimicrobial activity and cytotoxicity of the ethanol extract, fractions and eight compounds isolated from Eriosema robustum (Faba-ceae). BMC Complementary and Alternative Medicine 2013; 13: 289.
There are 21 citations in total.

Details

Primary Language English
Subjects Clinical Sciences
Journal Section Research Articles
Authors

Betül Özaltun This is me

Taner Daştan

Publication Date December 1, 2019
Submission Date March 1, 2019
Acceptance Date May 3, 2019
Published in Issue Year 2019 Volume: 26 Issue: 4

Cite

Vancouver Özaltun B, Daştan T. Evaluation of Antimicrobial Activities and In Vitro Cytotoxic Activities of Gundelia tournefortii L. Plant Extracts. Med J SDU. 2019;26(4):436-42.

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