Can dynamic thiol-disulfide homeostasis be an effective marker in the diagnosis of nodular goiter and thyroid cancer?
Abstract
Aim: The oxidative stress has an important role in thyroid pathologies by nature of thyroid gland. Dynamic thiol-disulfide homeostasis is one of the markers of oxidative stress and its counterpart antioxidants in the body. In our study, the dynamic thiol-disulfide homeostasis was investigated in cases underwent surgery due to thyroid cancer or nodular goiter.
Material and Methods: The study included patients who underwent thyroidectomy in General Surgery Department of Keçiören Teaching and Research Hospital between 01.03.2017 and 01.06.2017. The patients were assigned into groups according to postoperative histopathological examination: group 1 included patients with benign lesion in histopathology report and group 2 included patients with malignant lesions in histopathology report. The patients who had no pathology in sonography and did not undergo surgery were assigned into group 3 as controls. In all patients, venous blood samples were drawn to evaluate dynamic thiol-disulfide homeostasis before surgery.
Results: In the study, 98 cases underwent bilateral total thyroidectomy; 77 of which had benign disease and 21 of which had malignant disease. Native thiol values (µmol/L) were 317.4±4.2, 349.9±7.9 and 299.9±7.9 (p=0) while total thiol values (µmol/L) were 353.5±4.8, 386.5±9.5 and 332.6±8.3 (p=0) and disulfide values (µmol/L) were 18.4±0.5, 20.5±0.7 and 16.7±0.6 (p=0) in group 1 (benign disease), group 2 (malignant disease) and group 3 (controls), respectively. In addition disulfide: native thiol was 5.8±0.1, 5.9±0.2 and 5.7±0.2 (p=0.8) while disulfide: total thiol was 5.2±0.1, 5.4±0.2 and 5.1±0.2 and native thiol: total thiol was 89.9±0.5, 90.7±0.5 and 90.5±1.5 (p=0.4) in group 1, 2 and 3, respectively.
Conclusion: The dynamic thiol-disulfide homeostasis can be used as a marked in the thyroid diseases; however, further studies with larger sample are needed.
Keywords
References
- Paschke R , Hegedüs L, Alexander E, Valcavi R, Papini E, Gharib H. Thyroid nodule guidelines: agreement, disagreement and need for future research. Nat Rev Endocrinol. 2011 Jun;7(6):354-61. Doi: 10.1038/nrendo.2011.1. Epub 2011 Mar
- Kochman J , Jakubczyk K, Bargiel P, Janda-Milczarek K. The Influence of Oxidative Stress on Thyroid Diseases. Antioxidants (Basel). 2021 Sep 10;10(9):1442. doi: 10.3390/antiox10091442.
- El Hassani, R.A.; Buffet, C.; Leboulleux, S.; Dupuy, C. Oxidative stress in thyroid carcinomas: Biological and clinical significance. Endocr.-Relat. Cancer 2019, 26, R131–R143.
- ALKURT, E. G., TURHAN, S., ŞAHİN, F., & TUTAN, M. B. (2022). Papiller Tiroid Kanserli Hastalarda Preoperatif Santral Lenf Nodu Metastazının Öngörücüsü Olarak Glikoz-Lenfosit Oranı. Turkish Journal of Clinics and Laboratory, 13(4), 534-539.
- Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clinical Biochemistry. 2014;47(18):326–332. doi: 10.1016/j.clinbiochem.2014.09.026.
- Ünsal, A., Yavuz, A., Buluş, H., Aydın, A., Alışık, M., Erel, Ö., & Aydın Sr, A. (2022). Comparison of Thiol-Disulfide Hemostasis With Inflammatory Markers in the Inflammatory Process of Acute Appendicitis. Cureus, 14(9).
- Song, Y.; Driessens, N.; Costa, M.; De Deken, X.; Detours, V.; Corvilain, B.; Maenhaut, C.; Miot, F.; Van Sande, J.; Many, M.-C.; et al. Roles of Hydrogen Peroxide in Thyroid Physiology and Disease. J. Clin. Endocrinol. Metab. 2007
- Paschke, R. Molecular pathogenesis of nodular goiter. Langenbecks Arch. Surg. 396, 1127–1136 (2011).
- Muzza, M.; Pogliaghi, G.; Colombo, C.; Carbone, E.; Cirello, V.; Palazzo, S.; Frattini, F.; Gentilini, D.; Gazzano, G.; Persani, L.; et al. Oxidative Stress Correlates with More Aggressive Features in Thyroid Cancer. Cancers 2022, 14, 5857
- Sugawara M, Kita T, Lee ED, Takamatsu J, Hagen GA, Kuma K, Medeiros-Neto GA. Deficiency of superoxide dismutase in endemic goiter tissue. J Clin Endocr Metab. 1988;67:1156-61.
- Durak I, Bayram F, Kavutcu M, Canbolat O, Oztürk HS. Impaired enzymatic antioxidant defence mechanism in cancerous human thyroid tissues. J Endocrinol Invest. 1996;19:312-5
- Akinci, M.; Kosova, F.; Çetin, B.; Sepici, A.; Altan, N.; Aslan, S.; Cetin, A.; Çetin, B. Oxidant/antioxidant balance in patients with thyroid cancer. Acta Cir. Bras. 2008, 23, 551–554.
- Bilginer MC,Tam AA, Fakı S, Güler BY, Erel Ö,Kılınç İ , Özdemir D, Topaloğlu O, Ersoy R, Çakır B. Association of thiol/disulphide homeostasis with Bethesda classification of thyroid nodules and thyroid cancer. Turk J Med Sci. 2022; 52(4): 990–996. doi: 10.55730/1300-0144.5400
Dinamik tiyol-disülfür homeostazisi nodüler guatr ve tiroid kanseri tanısında etkili bir belirteç olabilir mi?
Abstract
Amaç: Troid glandı doğası gereği troid patolojilerinde oxidatif stresin rolü önemlidir. Dinamik thiol-disulfit dengesi vücuttaki oksidatif stres ve onun dengeleyicisi antioksidanların göstergelerinden birisidir. Çalışmamızda Troid kanseri veya nodüler guatr nedeniyle opere edilen olgularda Dinamik thiol disulfit dengesi düzeylerini araştırdık.
Gereç ve Yöntemler: Keçiören Eğitim ve Araştırma hastanesi Genel Cerrahi kliniğinde 01.03.2017-01.06.2017 tarihleri arasında tiroidektomi operasyonu olan hastalar çalışmaya dahil edildi. Ameliyat sonrası patoloji sonuçları Benign gelenler Grup1, Malign gelenler Grup 2 ve Ultrasonda patoloji saptanmayan ve ameliyat edilmeyen olgular ise Grup 3 Kontrol grubuna dahil edildi. Çalışma grubundan ameliyat öncesi olmak üzere Dinamik thiol-disulfit dengesinin araştırılması için venöz kan örnekleri alındı.
Bulgular: 98 hastaya bilateral total tiroidektomi ameliyatı uygulandı; bunlardan, 77 hastanın patoloji sonuçları benign, 21 hastanın ise malign rapor edildi. Grup 1(benign), Grup 2 (malign) ve Grup 3 (kontrol) gruplarında sırasıyla Native thiol değerleri (µmol/L) 317.4± 4.2, 349.9± 7.9 , 299.9± 7.9 (p=0), Total Thiol değerleri (µmol/L); 353.5.0±4.8, 386.5±9.5, 332.6± 8.3 (p=0), Disülfit (µmol/L); 18.4 ±0.5 20.5±0.7, 16.7±0.6 (p=0), Disulphide/native thiol 5.8.0±0.1, 5.9±0.2, 5.7± 0.2 (p=0.8), Disulphide/total thiol 5.2±0.1, 5.4±0.2, 5.1± 0.2 (p=0.7) ve Native thiol/total 89.9±0.5, 90.7±0.5, 90.5± 1.5 (p=0.4) olarak saptandı.
Sonuç: Tiroid hastalıkları tanısında Dinamik thiol-disulfit dengesi bir belirteç olarak kullanılabilir ancak geniş serili çalışmalara ihtiyaç duyulmaktadır.
Keywords
References
- Paschke R , Hegedüs L, Alexander E, Valcavi R, Papini E, Gharib H. Thyroid nodule guidelines: agreement, disagreement and need for future research. Nat Rev Endocrinol. 2011 Jun;7(6):354-61. Doi: 10.1038/nrendo.2011.1. Epub 2011 Mar
- Kochman J , Jakubczyk K, Bargiel P, Janda-Milczarek K. The Influence of Oxidative Stress on Thyroid Diseases. Antioxidants (Basel). 2021 Sep 10;10(9):1442. doi: 10.3390/antiox10091442.
- El Hassani, R.A.; Buffet, C.; Leboulleux, S.; Dupuy, C. Oxidative stress in thyroid carcinomas: Biological and clinical significance. Endocr.-Relat. Cancer 2019, 26, R131–R143.
- ALKURT, E. G., TURHAN, S., ŞAHİN, F., & TUTAN, M. B. (2022). Papiller Tiroid Kanserli Hastalarda Preoperatif Santral Lenf Nodu Metastazının Öngörücüsü Olarak Glikoz-Lenfosit Oranı. Turkish Journal of Clinics and Laboratory, 13(4), 534-539.
- Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clinical Biochemistry. 2014;47(18):326–332. doi: 10.1016/j.clinbiochem.2014.09.026.
- Ünsal, A., Yavuz, A., Buluş, H., Aydın, A., Alışık, M., Erel, Ö., & Aydın Sr, A. (2022). Comparison of Thiol-Disulfide Hemostasis With Inflammatory Markers in the Inflammatory Process of Acute Appendicitis. Cureus, 14(9).
- Song, Y.; Driessens, N.; Costa, M.; De Deken, X.; Detours, V.; Corvilain, B.; Maenhaut, C.; Miot, F.; Van Sande, J.; Many, M.-C.; et al. Roles of Hydrogen Peroxide in Thyroid Physiology and Disease. J. Clin. Endocrinol. Metab. 2007
- Paschke, R. Molecular pathogenesis of nodular goiter. Langenbecks Arch. Surg. 396, 1127–1136 (2011).
- Muzza, M.; Pogliaghi, G.; Colombo, C.; Carbone, E.; Cirello, V.; Palazzo, S.; Frattini, F.; Gentilini, D.; Gazzano, G.; Persani, L.; et al. Oxidative Stress Correlates with More Aggressive Features in Thyroid Cancer. Cancers 2022, 14, 5857
- Sugawara M, Kita T, Lee ED, Takamatsu J, Hagen GA, Kuma K, Medeiros-Neto GA. Deficiency of superoxide dismutase in endemic goiter tissue. J Clin Endocr Metab. 1988;67:1156-61.
- Durak I, Bayram F, Kavutcu M, Canbolat O, Oztürk HS. Impaired enzymatic antioxidant defence mechanism in cancerous human thyroid tissues. J Endocrinol Invest. 1996;19:312-5
- Akinci, M.; Kosova, F.; Çetin, B.; Sepici, A.; Altan, N.; Aslan, S.; Cetin, A.; Çetin, B. Oxidant/antioxidant balance in patients with thyroid cancer. Acta Cir. Bras. 2008, 23, 551–554.
- Bilginer MC,Tam AA, Fakı S, Güler BY, Erel Ö,Kılınç İ , Özdemir D, Topaloğlu O, Ersoy R, Çakır B. Association of thiol/disulphide homeostasis with Bethesda classification of thyroid nodules and thyroid cancer. Turk J Med Sci. 2022; 52(4): 990–996. doi: 10.55730/1300-0144.5400
Details
| Primary Language | English |
|---|---|
| Subjects | General Surgery |
| Journal Section | Research Article |
| Authors | |
| Publication Date | September 30, 2023 |
| Published in Issue | Year 2023 Volume: 14 Issue: 3 |
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e-ISSN: 2149-8296
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