Transkraniyal doğru akım stimülasyonunun deneysel hafif travmatik beyin hasarının neden olduğu motor fonksiyon kaybı üzerine terapötik etkileri
Öz
Amaç: Travmatik beyin hasarı (TBH) lokomotor aktivite, öğrenme ve hafıza gibi davranışsal bozukluklara neden olan ciddi bir hastalıktır. Bu çalışmanın amacı, deneysel travmatik beyin hasarı modelinde transkraniyal Doğru Akım Stimülasyonu (tDAS) tedavisinin lokomotor aktivite üzerindeki davranışsal etkilerini araştırmak ve motor korteks dokusunda IL-1β ve IL-18 düzeylerini incelemektir.
Gereç ve Yöntem: 30 adet 3 aylık erkek Wistar albino sıçanlar kullanıldı. TBH modeli Marmarou yöntemi kullanılarak oluşturuldu. Sham ve TBH+tDAS gruplarına TBH'nın 2. saatinde başlanıp 2 gün boyunca günde 0.5 mA 30 dakika anodal tDAS tedavisi uygulandı. Lokomotor aktivite açık alan testinde değerlendirildi. Motor korteks dokusunda IL-1β ve IL-18 düzeyleri ELİSA yöntemi ile ölçüldü.
Bulgular: Lokomotor aktivite sonuçları sham grubuna kıyasla TBH grubunda anlamlı düzeyde azalma görülürken), TBH+tDAS grubunda TBH grubuna kıyasla anlamlı artma görüldü. TBH grubunun motor korteks IL-1β ve IL-18 değerlerinde sham grubuna kıyasla anlamlı artma tespit edilirken, TBH+tDAS grubunda TBH grubuna kıyasla anlamlı bir azalam tespit edildi.
Sonuç: Çalışmamızda, tDAS tedavisinin travmatik beyin hasarına karşı nöroinflamasyon üzerinde terapötik etkiye sahip olduğu gösterildi.
Anahtar Kelimeler
Motor fonksiyon terapötik tedavi travmatik beyin hasarı transkraniyal doğru akım stimülasyonu
Proje Numarası
Project number: TIP19001.21.003
Kaynakça
- Akçay G. Weight drop models in traumatic brain injury. Mid Blac Sea J Health Sci. 2023;9:375-84.
- Capizzi A, Woo J, Verduzco-Gutierrez M. Traumatic brain injury: An overview of epidemiology, pathophysiology, and medical management. Med Clin North Am. 2020;104:213-38.
- Galgano M, Toshkezi G, Qiu X, Russell T, Chin L, Zhao LR. Traumatic brain injury: current treatment strategies and future endeavors. Cell Transplant. 2017;26:1118-30.
- Maas AI, Stocchetti N, Bullock R. Moderate and severe traumatic brain injury in adults. Lancet Neurol. 2008;7:728-41.
- Yi JH, Hazell AS. Excitotoxic mechanisms and the role of astrocytic glutamate transporters in traumatic brain injury. Neurochem Int. 2006;48:394-403.
- Dirnagl U, Iadecola C, Moskowitz MA. Pathobiology of ischaemic stroke: an integrated view. Trends Neurosci. 1999;22:391-7.
- Yang B, Zhang R, Sa Q, Du Y. Rhamnazin ameliorates traumatic brain injury in mice via reduction in apoptosis, oxidative stress, and inflammation. Neuroimmunomodulation. 2022;29:28-35.
- Rana A, Singh S, Deshmukh R, Kumar A. Pharmacological potential of tocopherol and doxycycline against traumatic brain injury-induced cognitive/motor impairment in rats. Brain Inj. 2020;34:1039-50.
- Wang H, Lynch JR, Song P, et al. Simvastatin and atorvastatin improve behavioral outcome, reduce hippocampal degeneration, and improve cerebral blood flow after experimental traumatic brain injury. Exp Neurol. 2007;206:59-69.
- Benninger DH, Lomarev M, Lopez G, et al. Transcranial direct current stimulation for the treatment of Parkinson's disease. J Neurol Neurosurg Psychiatry. 2010;81:1105-11.
- Purpura DP, McMurtry JG. Intracellular activities and evoked potential changes during polarization of motor cortex. J Neurophysiol. 1965;28:166-85.
- Bashir S, Yoo W-K. Neuromodulation for addiction by transcranial direct current stimulation: opportunities and challenges. Ann Neurosci. 2016;23:241-5.
- Stagg CJ, Antal A, Nitsche MA. Physiology of transcranial direct current stimulation. J ECT. 2018;34:144-52.
- Akcay G, Nemutlu Samur D, Derin N. Transcranial direct current stimulation alleviates nociceptive behavior in male rats with neuropathic pain by regulating oxidative stress and reducing neuroinflammation. J Neurosci Res. 2023;101:1457-70.
- Akcay G. Therapeutic effects of transcranial direct current stimulation on ketamine-induced schizophrenia-like behaviors and oxidative stress. Med Science. 2023;12:63-9.
- Akcay G. Investigation of the learning and memory enhancing effects of 0.25 ma and 0.5 ma anodal and cathodal transcranial direct current stimulations in healthy rats. Mid Blac Sea J Health Sci. 2023;9: 98 - 110.
- Han SJ, Park G, Suh JH. Transcranial direct current stimulation combined with amantadine in repetitive mild traumatic brain injury in rats. BMC Neurosci. 2022;23:76.
- Kim HJ, Han SJ. Anodal transcranial direct current stimulation provokes neuroplasticity in repetitive mild traumatic brain injury in rats. Neural Plast. 2017;2017:1372946.
- Yu KP, Yoon YS, Lee JG, et al. Effects of electric cortical stimulation (ecs) and transcranial direct current stimulation (tDCS) on rats with a traumatic brain injury. Ann Rehabil Med. 2018;42:502-13.
- Qi L, Cui X, Dong W, et al. Ghrelin attenuates brain injury after traumatic brain injury and uncontrolled hemorrhagic shock in rats. Mol Med. 2012;18:186-93.
- Marmarou A, Foda MA, van den Brink W, Campbell J, Kita H, Demetriadou K. A new model of diffuse brain injury in rats. Part I: Pathophysiology and biomechanics. J Neurosurg. 1994;80:291-300.
- McAteer KM, Corrigan F, Thornton E, Turner RJ, Vink R. Short and long term behavioral and pathological changes in a novel rodent model of repetitive mild traumatic brain injury. PLoS One. 2016;11:e0160220..
- Li Y, Zhang L, Kallakuri S, Zhou R, Cavanaugh JM. Quantitative relationship between axonal injury and mechanical response in a rodent head impact acceleration model. J Neurotrauma.2011;28:1767-82.
- Sahin K, Kilic E, Balcikanli Z, et al. Ginger provides neuroprotection in experimental model of traumatic brain injury. FASEB J. 2019;33(S1):795.16
Therapeutic effects of transcranial direct current stimulation on loss of motor function caused by experimental mild traumatic brain injury
Öz
Purpose: Traumatic brain injury (TBI) is a serious illness that causes behavioral disorders such as locomotor activity, learning, and memory. This study aims to investigate the behavioral effects of transcranial Direct Current Stimulation (tDCS) treatment on locomotor activity in an experimental traumatic brain injury model and to investigate the levels of IL-1β and IL-18 in the motor cortex tissue.
Materials and Methods: 30 male 3-month-old Wistar albino rats were used. The TBI model was established using the Marmarou method. 2 hours after TBI, sham and TBI+tDCS groups were treated with 0.5 mA 30 minutes anodal tDCS treatment for 2 days. Locomotor activity was evaluated in open field test. IL-1β and IL-18 levels in motor cortex tissue were measured by the ELISA method..
Results: Compared to the sham group, locomotor activity results showed significant decreases in the TBI group while the TBI+tDCS group showed significant increases compared to the TBI group. There were significant increases in IL-1β and IL-18 values in the motor cortex of the animals in the TBI group compared to the sham group, while there was a significant decrease in the TBI+tDCS group compared to the TBI group.
Conclusion: tDCS treatment was shown to have therapeutic effects on neuroinflammation against traumatic brain injury.
Anahtar Kelimeler
Motor Function Therapeutic Treatment Traumatic Brain Injury transcranial Direct Current Stimulation
Destekleyen Kurum
This work was supported by the Coordination Unit of Scientific Research Projects of Erciyes University (Project number: TIP19001.21.003)
Proje Numarası
Project number: TIP19001.21.003
Kaynakça
- Akçay G. Weight drop models in traumatic brain injury. Mid Blac Sea J Health Sci. 2023;9:375-84.
- Capizzi A, Woo J, Verduzco-Gutierrez M. Traumatic brain injury: An overview of epidemiology, pathophysiology, and medical management. Med Clin North Am. 2020;104:213-38.
- Galgano M, Toshkezi G, Qiu X, Russell T, Chin L, Zhao LR. Traumatic brain injury: current treatment strategies and future endeavors. Cell Transplant. 2017;26:1118-30.
- Maas AI, Stocchetti N, Bullock R. Moderate and severe traumatic brain injury in adults. Lancet Neurol. 2008;7:728-41.
- Yi JH, Hazell AS. Excitotoxic mechanisms and the role of astrocytic glutamate transporters in traumatic brain injury. Neurochem Int. 2006;48:394-403.
- Dirnagl U, Iadecola C, Moskowitz MA. Pathobiology of ischaemic stroke: an integrated view. Trends Neurosci. 1999;22:391-7.
- Yang B, Zhang R, Sa Q, Du Y. Rhamnazin ameliorates traumatic brain injury in mice via reduction in apoptosis, oxidative stress, and inflammation. Neuroimmunomodulation. 2022;29:28-35.
- Rana A, Singh S, Deshmukh R, Kumar A. Pharmacological potential of tocopherol and doxycycline against traumatic brain injury-induced cognitive/motor impairment in rats. Brain Inj. 2020;34:1039-50.
- Wang H, Lynch JR, Song P, et al. Simvastatin and atorvastatin improve behavioral outcome, reduce hippocampal degeneration, and improve cerebral blood flow after experimental traumatic brain injury. Exp Neurol. 2007;206:59-69.
- Benninger DH, Lomarev M, Lopez G, et al. Transcranial direct current stimulation for the treatment of Parkinson's disease. J Neurol Neurosurg Psychiatry. 2010;81:1105-11.
- Purpura DP, McMurtry JG. Intracellular activities and evoked potential changes during polarization of motor cortex. J Neurophysiol. 1965;28:166-85.
- Bashir S, Yoo W-K. Neuromodulation for addiction by transcranial direct current stimulation: opportunities and challenges. Ann Neurosci. 2016;23:241-5.
- Stagg CJ, Antal A, Nitsche MA. Physiology of transcranial direct current stimulation. J ECT. 2018;34:144-52.
- Akcay G, Nemutlu Samur D, Derin N. Transcranial direct current stimulation alleviates nociceptive behavior in male rats with neuropathic pain by regulating oxidative stress and reducing neuroinflammation. J Neurosci Res. 2023;101:1457-70.
- Akcay G. Therapeutic effects of transcranial direct current stimulation on ketamine-induced schizophrenia-like behaviors and oxidative stress. Med Science. 2023;12:63-9.
- Akcay G. Investigation of the learning and memory enhancing effects of 0.25 ma and 0.5 ma anodal and cathodal transcranial direct current stimulations in healthy rats. Mid Blac Sea J Health Sci. 2023;9: 98 - 110.
- Han SJ, Park G, Suh JH. Transcranial direct current stimulation combined with amantadine in repetitive mild traumatic brain injury in rats. BMC Neurosci. 2022;23:76.
- Kim HJ, Han SJ. Anodal transcranial direct current stimulation provokes neuroplasticity in repetitive mild traumatic brain injury in rats. Neural Plast. 2017;2017:1372946.
- Yu KP, Yoon YS, Lee JG, et al. Effects of electric cortical stimulation (ecs) and transcranial direct current stimulation (tDCS) on rats with a traumatic brain injury. Ann Rehabil Med. 2018;42:502-13.
- Qi L, Cui X, Dong W, et al. Ghrelin attenuates brain injury after traumatic brain injury and uncontrolled hemorrhagic shock in rats. Mol Med. 2012;18:186-93.
- Marmarou A, Foda MA, van den Brink W, Campbell J, Kita H, Demetriadou K. A new model of diffuse brain injury in rats. Part I: Pathophysiology and biomechanics. J Neurosurg. 1994;80:291-300.
- McAteer KM, Corrigan F, Thornton E, Turner RJ, Vink R. Short and long term behavioral and pathological changes in a novel rodent model of repetitive mild traumatic brain injury. PLoS One. 2016;11:e0160220..
- Li Y, Zhang L, Kallakuri S, Zhou R, Cavanaugh JM. Quantitative relationship between axonal injury and mechanical response in a rodent head impact acceleration model. J Neurotrauma.2011;28:1767-82.
- Sahin K, Kilic E, Balcikanli Z, et al. Ginger provides neuroprotection in experimental model of traumatic brain injury. FASEB J. 2019;33(S1):795.16
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Nöroloji ve Nöromüsküler Hastalıklar |
| Bölüm | Araştırma |
| Yazarlar | |
| Proje Numarası | Project number: TIP19001.21.003 |
| Erken Görünüm Tarihi | 25 Eylül 2023 |
| Yayımlanma Tarihi | 30 Eylül 2023 |
| Kabul Tarihi | 8 Eylül 2023 |
| Yayımlandığı Sayı | Yıl 2023 Cilt: 48 Sayı: 3 |
