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The Effects of Vasointestinal Peptide and Naringenin on Rotenone-Induced Experimental Model of Parkinson's Disease

Yıl 2023, Cilt: 25 Sayı: 2, 179 - 184, 30.08.2023

Ayşe Nur Yıldırım Ferhat Şirinyıldız Recep Özmerdivenli

https://doi.org/10.18678/dtfd.1301252

Öz

Aim: The aim of this study was to evaluate the intraperitoneal administration of naringenin and vasointestinal peptide (VIP), which are shown effective in various scientific studies, in terms of anti-Parkinsonian activity in rats.
Material and Methods: Forty-eight Wistar albino female rats were divided into 4 groups. No intervention was made in the control group, rotenone was given to the RT group, rotenone and VIP (25 ng/kg) to the RT+VIP group, and rotenone and naringenin (10 mg/kg) to the RT+NG group. All treatments were administered intraperitoneally for 14 days. The hole and board method was used to show the effects of the Parkinson's model on behavior. On the last day of the experiment, motor tests were carried out with the hole and board apparatus. After the study was completed, biochemical analyzes were performed from brain tissue samples.
Results: In comparison to the RT group, while the alpha-sync level in the RT+NG (p=0.023), malondialdehyde (MDA) levels both in the RT+VIP (p=0.039) and RT+NG (p=0.032), and superoxide dismutase (SOD) inhibition in the RT+VIP (p=0.042) groups decreased significantly, the 8-OHdG levels in the RT+VIP (p=0.042) and RT+NG (p=0.034) groups increased significantly. Statistically significant improvement was found both in biochemical and motor activities with the VIP and naringenin treatments applied.
Conclusion: According to the results obtained, the symptoms of Parkinson's disease were formed biochemically by rotenone application. The administration of VIP and naringenin treatments has shown positive effects experimentally and has been promising as an adjunct treatment element in the fight against Parkinson's disease.

Anahtar Kelimeler

Parkinson rats rotenone vasointestinal peptide

Destekleyen Kurum

ADÜ BAP

Proje Numarası

TPF-20015

Kaynakça

  • Alves da Costa C, Checler F. Apoptosis in Parkinson’s disease: is p53 the missing link between genetic and sporadic Parkinsonism? Cell Signal. 2011;23(6):963-8.
  • Dauer W, Przedborski S. Parkinson’s disease: mechanisms and models. Neuron. 2003;39(6):889-909.
  • Blandini F, Armentero MT. Animal models of Parkinson’s disease. FEBS J. 2012;279(7):1156-66.
  • Balestrino R, Schapira AHV. Parkinson disease. Eur J Neurol. 2020;27(1):27-42.
  • Poewe W, Seppi K, Tanner CM, Halliday GM, Brundin P, Volkmann J, et al. Parkinson disease. Nat Rev Dis Primer. 2017;3:17013.
  • Cannon JR, Tapias V, Na HM, Honick AS, Drolet RE, Greenamyre JT. A highly reproducible rotenone model of Parkinson’s disease. Neurobiol Dis. 2009;34(2):279-90.
  • Betarbet R, Sherer TB, MacKenzie G, Garcia-Osuna M, Panov AV, Greenamyre JT. Chronic systemic pesticide exposure reproduces features of Parkinson’s disease. Nat Neurosci. 2000;3(12):1301-6.
  • Bové J, Perier C. Neurotoxin-based models of Parkinson’s disease. Neuroscience. 2012;211:51-76.
  • Drechsel DA, Patel M. Role of reactive oxygen species in the neurotoxicity of environmental agents implicated in Parkinson’s disease. Free Radic Biol Med. 2008;44(11):1873-86.
  • Reeve A, Simcox E, Turnbull D. Ageing and Parkinson’s disease: why is advancing age the biggest risk factor? Ageing Res Rev. 2014;14(100):19-30.
  • Hu Q, Wang G. Mitochondrial dysfunction in Parkinson’s disease. Transl Neurodegener. 2016;5:14.
  • Harmar AJ, Fahrenkrug J, Gozes I, Laburthe M, May V, Pisegna JR, et al. Pharmacology and functions of receptors for vasoactive intestinal peptide and pituitary adenylate cyclase-activating polypeptide: IUPHAR review 1. Br J Pharmacol. 2012;166(1):4-17.
  • Tatemoto K, Mutt V. Isolation and characterization of the intestinal peptide porcine PHI (PHI-27), a new member of the glucagon--secretin family. Proc Natl Acad Sci USA. 1981;78(11):6603-7.
  • Tunçel N, Korkmaz OT, Tekin N, Şener E, Akyüz F, Inal M. Antioxidant and anti-apoptotic activity of vasoactive intestinal peptide (VIP) against 6-hydroxy dopamine toxicity in the rat corpus striatum. J Mol Neurosci. 2012;46(1):51-7.
  • Korkmaz O, Ay H, Ulupınar E, Tunçel N. Vasoactive intestinal peptide enhances striatal plasticity and prevents dopaminergic cell loss in Parkinsonian rats. J Mol Neurosci. 2012;48(3):565-73.
  • Masmoudi-Kouki O, Gandolfo P, Castel H, Leprince J, Fournier A, Dejda A, et al. Role of PACAP and VIP in astroglial functions. Peptides. 2007;28(9):1753-60.
  • Dogrukol-Ak D, Tore F, Tuncel N. Passage of VIP/PACAP/secretin family across the blood-brain barrier: therapeutic effects. Curr Pharm Des. 2004;10(12):1325-40.
  • Kalfin R, Maulik N, Engelman RM, Cordis GA, Milenov K, Kasakov L, et al. Protective role of intracoronary vasoactive intestinal peptide in ischemic and reperfused myocardium. J Pharmacol Exp Ther. 1994;268(2):952-8.
  • Delgado M, Ganea D. Vasoactive intestinal peptide: a neuropeptide with pleiotropic immune functions. Amino Acids. 2013;45(1):25-39.
  • Salehi B, Fokou PVT, Sharifi-Rad M, Zucca P, Pezzani R, Martins N, et al. The therapeutic potential of naringenin: a review of clinical trials. Pharmaceuticals (Basel). 2019;12(1):11.
  • Wilcox LJ, Borradaile NM, Huff MW. Antiatherogenic properties of naringenin, a citrus flavonoid. Cardiovasc Drug Rev. 1999;17(2):160-78.
  • Renugadevi J, Prabu SM. Naringenin protects against cadmium-induced oxidative renal dysfunction in rats. Toxicology. 2009;256(1-2):128-34.
  • Wang Q, Yang J, Zhang X, Zhou L, Liao XL, Yang B. Practical synthesis of naringenin. J Chem Res. 2015;39(8):455-7.
  • Jayachitra J, Nalini N. Effect of naringenin (citrus flavanone) on lipid profile in ethanol-induced toxicity in rats. J Food Biochem. 2012;36(4):502-11.
  • Yelkenli İH, Ulupinar E, Korkmaz OT, Şener E, Kuş G, Filiz Z, et al. Modulation of corpus striatal neurochemistry by astrocytes and vasoactive intestinal peptide (VIP) in parkinsonian rats. J Mol Neurosci. 2016;59(2):280-9.
  • Sonia Angeline M, Sarkar A, Anand K, Ambasta RK, Kumar P. Sesamol and naringenin reverse the effect of rotenone-induced PD rat model. Neuroscience. 2013;254:379-94.
  • Sonia Angeline M, Chaterjee P, Anand K, Ambasta RK, Kumar P. Rotenone-induced parkinsonism elicits behavioral impairments and differential expression of parkin, heat shock proteins and caspases in the rat. Neuroscience. 2012;220:291-301.
  • Lapointe N, St-Hilaire M, Martinoli MG, Blanchet J, Gould P, Rouillard C, et al. Rotenone induces non-specific central nervous system and systemic toxicity. FASEB J. 2004;18(6):717-9.
  • Vauzour D, Vafeiadou K, Rodriguez-Mateos A, Rendeiro C, Spencer JP. The neuroprotective potential of flavonoids: a multiplicity of effects. Genes Nutr. 2008;3(3-4):115-26.
  • Angeloni C, Vauzour D. Natural products and neuroprotection. Int J Mol Sci. 2019;20(22):5570.
  • Datla KP, Christidou M, Widmer WW, Rooprai HK, Dexter DT. Tissue distribution and neuroprotective effects of citrus flavonoid tangeretin in a rat model of Parkinson’s disease. Neuroreport. 2001;12(17):3871-5.
  • Brenneman DE. Neuroprotection: a comparative view of vasoactive intestinal peptide and pituitary adenylate cyclase-activating polypeptide. Peptides. 2007;28(9):1720-6.
  • Zupan V, Hill JM, Brenneman DE, Gozes I, Fridkin M, Robberecht P, et al. Involvement of pituitary adenylate cyclase-activating polypeptide II vasoactive intestinal peptide 2 receptor in mouse neocortical astrocytogenesis. J Neurochem. 1998;70(5):2165-73.
  • Liu KC, Li JY, Xie W, Li LB, Zhang J, Du CX, et al. Activation and blockade of serotonin6 receptors in the dorsal hippocampus enhance T maze and hole-board performance in a unilateral 6-hydroxydopamine rat model of Parkinson’s disease. Brain Res. 2016;1650:184-95.
  • Wang Y, Liu J, Hui Y, Wu Z, Wang L, Wu X, et al. Dose and time-dependence of acute intermittent theta-burst stimulation on hippocampus-dependent memory in parkinsonian rats. Front Neurosci. 2023;17:1124819.
  • Saleem U, Hussain L, Shahid F, Anwar F, Chauhdary Z, Zafar A. Pharmacological potential of the standardized methanolic extract of Prunus armeniaca L. in the haloperidol-induced parkinsonism rat model. Evid Based Complement Alternat Med. 2022;2022:3697522.
  • DeMaagd G, Philip A. Parkinson’s disease and its management: part 1: disease entity, risk factors, pathophysiology, clinical presentation, and diagnosis. Pharm Ther. 2015;40(8):504-32.

Vazointestinal Peptid ve Naringeninin Rotenon Kaynaklı Deneysel Parkinson Hastalığı Modeli Üzerine Etkileri

Yıl 2023, Cilt: 25 Sayı: 2, 179 - 184, 30.08.2023

Ayşe Nur Yıldırım Ferhat Şirinyıldız Recep Özmerdivenli

https://doi.org/10.18678/dtfd.1301252

Öz

Amaç: Bu çalışmanın amacı, çeşitli bilimsel çalışmalar ile etkili oldukları gösterilmiş olan naringenin ve vazointestinal peptidin (VIP) intraperitoneal olarak uygulanmasının ratlarda anti-Parkinson aktivitesi açısından değerlendirilmesidir.
Gereç ve Yöntemler: Kırk sekiz adet Wistar albino dişi rat 4 gruba ayrıldı. Kontrol grubuna herhangi bir müdahale yapılmadı, RT grubuna rotenon verilirken, RT+VIP grubuna rotenon ve VIP (25 ng/kg) ve RT+NG grubuna ise rotenone ve naringenin (10 mg/kg) verildi. Tüm tedaviler 14 gün süreyle intraperitoneal yolla uygulandı. Parkinson modelinin davranış üzerindeki etkilerini göstermek için hole and board yöntemi kullanıldı. Deneyin son günü hole and board aparatı ile motor testleri yapıldı. Çalışma tamamlandıktan sonra alınan beyin dokusu örneklerinden biyokimyasal analizler yapıldı.
Bulgular: RT grubuyla karşılaştırıldığında, RT+NG (p=0,023) grubunda alfa senkronizasyon düzeyi, hem RT+VIP (p=0,039) hem de RT+NG (p=0,032) gruplarında malondialdehit (MDA) düzeyleri ve RT+VIP (p=0,042) grubunda süperoksit dismutaz (SOD) inhibisyonu anlamlı olarak azalırken, RT+VIP (p=0,042) ve RT+NG (p=0,034) gruplarında ise 8-OHdG seviyeleri anlamlı şekilde arttı. Uygulanan VIP ve naringenin tedavileri ile hem biyokimyasal ve hem de motor aktivitelerinde istatistiksel olarak anlamlı şekilde düzelme saptandı.
Sonuç: Elde edilen sonuçlara göre rotenon uygulaması ile Parkinson hastalığının semptomları biyokimyasal olarak oluşturulmuştur. VIP ve naringenin tedavilerinin uygulanması deneysel olarak olumlu etkiler göstermiştir ve Parkinson hastalığı ile mücadelede yardımcı bir tedavi unsuru olarak umut verici olmuştur.

Anahtar Kelimeler

Parkinson hastalığı rotenon sıçanlar vazointestinal peptit

Proje Numarası

TPF-20015

Kaynakça

  • Alves da Costa C, Checler F. Apoptosis in Parkinson’s disease: is p53 the missing link between genetic and sporadic Parkinsonism? Cell Signal. 2011;23(6):963-8.
  • Dauer W, Przedborski S. Parkinson’s disease: mechanisms and models. Neuron. 2003;39(6):889-909.
  • Blandini F, Armentero MT. Animal models of Parkinson’s disease. FEBS J. 2012;279(7):1156-66.
  • Balestrino R, Schapira AHV. Parkinson disease. Eur J Neurol. 2020;27(1):27-42.
  • Poewe W, Seppi K, Tanner CM, Halliday GM, Brundin P, Volkmann J, et al. Parkinson disease. Nat Rev Dis Primer. 2017;3:17013.
  • Cannon JR, Tapias V, Na HM, Honick AS, Drolet RE, Greenamyre JT. A highly reproducible rotenone model of Parkinson’s disease. Neurobiol Dis. 2009;34(2):279-90.
  • Betarbet R, Sherer TB, MacKenzie G, Garcia-Osuna M, Panov AV, Greenamyre JT. Chronic systemic pesticide exposure reproduces features of Parkinson’s disease. Nat Neurosci. 2000;3(12):1301-6.
  • Bové J, Perier C. Neurotoxin-based models of Parkinson’s disease. Neuroscience. 2012;211:51-76.
  • Drechsel DA, Patel M. Role of reactive oxygen species in the neurotoxicity of environmental agents implicated in Parkinson’s disease. Free Radic Biol Med. 2008;44(11):1873-86.
  • Reeve A, Simcox E, Turnbull D. Ageing and Parkinson’s disease: why is advancing age the biggest risk factor? Ageing Res Rev. 2014;14(100):19-30.
  • Hu Q, Wang G. Mitochondrial dysfunction in Parkinson’s disease. Transl Neurodegener. 2016;5:14.
  • Harmar AJ, Fahrenkrug J, Gozes I, Laburthe M, May V, Pisegna JR, et al. Pharmacology and functions of receptors for vasoactive intestinal peptide and pituitary adenylate cyclase-activating polypeptide: IUPHAR review 1. Br J Pharmacol. 2012;166(1):4-17.
  • Tatemoto K, Mutt V. Isolation and characterization of the intestinal peptide porcine PHI (PHI-27), a new member of the glucagon--secretin family. Proc Natl Acad Sci USA. 1981;78(11):6603-7.
  • Tunçel N, Korkmaz OT, Tekin N, Şener E, Akyüz F, Inal M. Antioxidant and anti-apoptotic activity of vasoactive intestinal peptide (VIP) against 6-hydroxy dopamine toxicity in the rat corpus striatum. J Mol Neurosci. 2012;46(1):51-7.
  • Korkmaz O, Ay H, Ulupınar E, Tunçel N. Vasoactive intestinal peptide enhances striatal plasticity and prevents dopaminergic cell loss in Parkinsonian rats. J Mol Neurosci. 2012;48(3):565-73.
  • Masmoudi-Kouki O, Gandolfo P, Castel H, Leprince J, Fournier A, Dejda A, et al. Role of PACAP and VIP in astroglial functions. Peptides. 2007;28(9):1753-60.
  • Dogrukol-Ak D, Tore F, Tuncel N. Passage of VIP/PACAP/secretin family across the blood-brain barrier: therapeutic effects. Curr Pharm Des. 2004;10(12):1325-40.
  • Kalfin R, Maulik N, Engelman RM, Cordis GA, Milenov K, Kasakov L, et al. Protective role of intracoronary vasoactive intestinal peptide in ischemic and reperfused myocardium. J Pharmacol Exp Ther. 1994;268(2):952-8.
  • Delgado M, Ganea D. Vasoactive intestinal peptide: a neuropeptide with pleiotropic immune functions. Amino Acids. 2013;45(1):25-39.
  • Salehi B, Fokou PVT, Sharifi-Rad M, Zucca P, Pezzani R, Martins N, et al. The therapeutic potential of naringenin: a review of clinical trials. Pharmaceuticals (Basel). 2019;12(1):11.
  • Wilcox LJ, Borradaile NM, Huff MW. Antiatherogenic properties of naringenin, a citrus flavonoid. Cardiovasc Drug Rev. 1999;17(2):160-78.
  • Renugadevi J, Prabu SM. Naringenin protects against cadmium-induced oxidative renal dysfunction in rats. Toxicology. 2009;256(1-2):128-34.
  • Wang Q, Yang J, Zhang X, Zhou L, Liao XL, Yang B. Practical synthesis of naringenin. J Chem Res. 2015;39(8):455-7.
  • Jayachitra J, Nalini N. Effect of naringenin (citrus flavanone) on lipid profile in ethanol-induced toxicity in rats. J Food Biochem. 2012;36(4):502-11.
  • Yelkenli İH, Ulupinar E, Korkmaz OT, Şener E, Kuş G, Filiz Z, et al. Modulation of corpus striatal neurochemistry by astrocytes and vasoactive intestinal peptide (VIP) in parkinsonian rats. J Mol Neurosci. 2016;59(2):280-9.
  • Sonia Angeline M, Sarkar A, Anand K, Ambasta RK, Kumar P. Sesamol and naringenin reverse the effect of rotenone-induced PD rat model. Neuroscience. 2013;254:379-94.
  • Sonia Angeline M, Chaterjee P, Anand K, Ambasta RK, Kumar P. Rotenone-induced parkinsonism elicits behavioral impairments and differential expression of parkin, heat shock proteins and caspases in the rat. Neuroscience. 2012;220:291-301.
  • Lapointe N, St-Hilaire M, Martinoli MG, Blanchet J, Gould P, Rouillard C, et al. Rotenone induces non-specific central nervous system and systemic toxicity. FASEB J. 2004;18(6):717-9.
  • Vauzour D, Vafeiadou K, Rodriguez-Mateos A, Rendeiro C, Spencer JP. The neuroprotective potential of flavonoids: a multiplicity of effects. Genes Nutr. 2008;3(3-4):115-26.
  • Angeloni C, Vauzour D. Natural products and neuroprotection. Int J Mol Sci. 2019;20(22):5570.
  • Datla KP, Christidou M, Widmer WW, Rooprai HK, Dexter DT. Tissue distribution and neuroprotective effects of citrus flavonoid tangeretin in a rat model of Parkinson’s disease. Neuroreport. 2001;12(17):3871-5.
  • Brenneman DE. Neuroprotection: a comparative view of vasoactive intestinal peptide and pituitary adenylate cyclase-activating polypeptide. Peptides. 2007;28(9):1720-6.
  • Zupan V, Hill JM, Brenneman DE, Gozes I, Fridkin M, Robberecht P, et al. Involvement of pituitary adenylate cyclase-activating polypeptide II vasoactive intestinal peptide 2 receptor in mouse neocortical astrocytogenesis. J Neurochem. 1998;70(5):2165-73.
  • Liu KC, Li JY, Xie W, Li LB, Zhang J, Du CX, et al. Activation and blockade of serotonin6 receptors in the dorsal hippocampus enhance T maze and hole-board performance in a unilateral 6-hydroxydopamine rat model of Parkinson’s disease. Brain Res. 2016;1650:184-95.
  • Wang Y, Liu J, Hui Y, Wu Z, Wang L, Wu X, et al. Dose and time-dependence of acute intermittent theta-burst stimulation on hippocampus-dependent memory in parkinsonian rats. Front Neurosci. 2023;17:1124819.
  • Saleem U, Hussain L, Shahid F, Anwar F, Chauhdary Z, Zafar A. Pharmacological potential of the standardized methanolic extract of Prunus armeniaca L. in the haloperidol-induced parkinsonism rat model. Evid Based Complement Alternat Med. 2022;2022:3697522.
  • DeMaagd G, Philip A. Parkinson’s disease and its management: part 1: disease entity, risk factors, pathophysiology, clinical presentation, and diagnosis. Pharm Ther. 2015;40(8):504-32.
Toplam 37 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Klinik Tıp Bilimleri
Bölüm Araştırma Makalesi
Yazarlar

Ayşe Nur Yıldırım 0000-0001-7587-3904

Ferhat Şirinyıldız 0000-0001-8800-9787

Recep Özmerdivenli 0000-0001-6458-5296

Proje Numarası TPF-20015
Erken Görünüm Tarihi 15 Ağustos 2023
Yayımlanma Tarihi 30 Ağustos 2023
Gönderilme Tarihi 23 Mayıs 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 25 Sayı: 2

Kaynak Göster

APA Yıldırım, A. N., Şirinyıldız, F., & Özmerdivenli, R. (2023). The Effects of Vasointestinal Peptide and Naringenin on Rotenone-Induced Experimental Model of Parkinson’s Disease. Duzce Medical Journal, 25(2), 179-184. https://doi.org/10.18678/dtfd.1301252
AMA Yıldırım AN, Şirinyıldız F, Özmerdivenli R. The Effects of Vasointestinal Peptide and Naringenin on Rotenone-Induced Experimental Model of Parkinson’s Disease. Duzce Med J. Ağustos 2023;25(2):179-184. doi:10.18678/dtfd.1301252
Chicago Yıldırım, Ayşe Nur, Ferhat Şirinyıldız, ve Recep Özmerdivenli. “The Effects of Vasointestinal Peptide and Naringenin on Rotenone-Induced Experimental Model of Parkinson’s Disease”. Duzce Medical Journal 25, sy. 2 (Ağustos 2023): 179-84. https://doi.org/10.18678/dtfd.1301252.
EndNote Yıldırım AN, Şirinyıldız F, Özmerdivenli R (01 Ağustos 2023) The Effects of Vasointestinal Peptide and Naringenin on Rotenone-Induced Experimental Model of Parkinson’s Disease. Duzce Medical Journal 25 2 179–184.
IEEE A. N. Yıldırım, F. Şirinyıldız, ve R. Özmerdivenli, “The Effects of Vasointestinal Peptide and Naringenin on Rotenone-Induced Experimental Model of Parkinson’s Disease”, Duzce Med J, c. 25, sy. 2, ss. 179–184, 2023, doi: 10.18678/dtfd.1301252.
ISNAD Yıldırım, Ayşe Nur vd. “The Effects of Vasointestinal Peptide and Naringenin on Rotenone-Induced Experimental Model of Parkinson’s Disease”. Duzce Medical Journal 25/2 (Ağustos 2023), 179-184. https://doi.org/10.18678/dtfd.1301252.
JAMA Yıldırım AN, Şirinyıldız F, Özmerdivenli R. The Effects of Vasointestinal Peptide and Naringenin on Rotenone-Induced Experimental Model of Parkinson’s Disease. Duzce Med J. 2023;25:179–184.
MLA Yıldırım, Ayşe Nur vd. “The Effects of Vasointestinal Peptide and Naringenin on Rotenone-Induced Experimental Model of Parkinson’s Disease”. Duzce Medical Journal, c. 25, sy. 2, 2023, ss. 179-84, doi:10.18678/dtfd.1301252.
Vancouver Yıldırım AN, Şirinyıldız F, Özmerdivenli R. The Effects of Vasointestinal Peptide and Naringenin on Rotenone-Induced Experimental Model of Parkinson’s Disease. Duzce Med J. 2023;25(2):179-84.
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