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Toprak Streptomyces İzolatlarının Cryptococcus neoformans Kültürlerine Karşı Antifungal Aktivitesi

Yıl 2021, Cilt: 9 Sayı: 2, 591 - 595, 25.04.2021

Gorkem Dulger Başaran Dülger

https://doi.org/10.29130/dubited.862551

Öz

Bu çalışmada, Düzce ili topraklardan elde edilen 128 farklı Streptomyces izolatlarının % 26,5'inin Cryptococcus neoformans ATCC 90112 mayasına karşı antifungal aktivite gösterdiği saptanmıştır. Agar plağında oluşan inhibisyon bölgesinin çapı dikkate alınarak izolatlar dört bölüme ayrılmıştır. Buna göre, bölüm 1 (5-10 mm, hafif aktif); bölüm 2 (11-15 mm, orta derecede aktif), bölüm 3 (16-25 mm, yüksek derecede aktif) ve bölüm 4 (26-35 mm, ultra aktif) olarak belirlenmiştir. Bölüm 4'teki 3 izolatın ise kriptokokkoz hastalığına karşı yeni bir antibiyotik kaynağı olabileceği saptanmıştır.




Anahtar Kelimeler

Streptomyces Antifungal Aktivite Cryptococcus neoformans

Kaynakça

  • [1] G. Gebreselema, M. Feleke, S. Samuel, R. Nagappan, “Isolation and characterization of potential antibiotic producing Actinomycetes from water and sediments of Lake Tana Ethiopia”, Asian Pacific Journal of Tropical Biomedicine, vol. 3, pp. 426-35, 2013.
  • [2] J. Berdy, “Bioactive microbial metabolites: a personal view”, The Journal of Antibiotics, vol. 58(1), pp. 1-26, 2005.
  • [3] I. Saadoun, F. Al-Momani, “Steptomycetes from Jordan soils active against Agrobacterium tumefaciens”, Actinomycetes, vol. 8, pp. 29-36, 1997.
  • [4] A.W. Bauer, W.M. Kirby, J.C. Sherris, M. Turk, “Antibiotic susceptibility testing by a standardized single disk method”, American Journal of Clinical Pathology, vol. 45, pp. 493-496, 1966.
  • [5] H.M. Atta, “Production, purification, physico-chemical characteristics and biological activities of antifungal antibiotic produced by Streptomyces antibioticus, AZ-Z710”, American-Eurasian Journal of Scientific Research, vol. 5(1), pp. 39-49, 2010.
  • [6] S. Ramesh, N. Mathivanan, “Screening of marine Actinomycetes isolated from the Bay of Bengal, India for antimicrobial activity and industrial enzymes”, World Journal of Microbiology and Biotechnology, vol. 25, pp. 2103–2111, 2009
  • [7] P.R. Jensen, P.G. Williams, D.C. Oh, L. Zeigler, W. Fenical, “Species-specific secondary metabolite production in marine Actinomycetes of the genus Salinispora”, Applied and Environmental Microbiology, vol. 73, pp. 1146–1152, 2007.
  • [8] A.A. Denizci, “Ege ve Doğu Karadeniz bölgesi topraklarinden izole edilen aktinomisetlerden antibakteriyal antibiyotiklerin aranması ve üretimi üzerine bir araştırma”, Doctoral Tezi, Ege Üniversitesi, Fen Bilimleri Enstitisü, 1996.
  • [9] N. Sahin, A. Ugur, “Investigation of the antimicrobial activity of some Streptomyces isolates”, Turkish Journal of Biology, vol. 27, pp. 79-84, 2003.
  • [10] M. Mohseni, H. Norouzi, J. Hamedi, A. Roohi, “Screening of antibacterial producing actinomycetes from sediments of the Caspian Sea”, International Journal of Molecular and Cellular Medicine, vol. 2(2), pp. 65-71, 2013.
  • [11] C. Coelho, A.L. Bocca, A. Casadevall, “The tools for virulence of Cryptococcus neoformans”, Advances in Applied Microbiology, vol. 87, pp. 1-41, 2014.
  • [12] R. Inci, “Kriptokok infeksiyonları”, Flora, vol. 13(2), pp. 61-71, 2008.

Antifungal Activity of Soil Streptomyces Isolates Against Cryptococcus neoformans

Yıl 2021, Cilt: 9 Sayı: 2, 591 - 595, 25.04.2021

Gorkem Dulger Başaran Dülger

https://doi.org/10.29130/dubited.862551

Öz

In this study, 26.5% of 128 different isolates of Streptomyces recovered from soils in Duzce province, Turkey showed antifungal activity against Cryptococcus neoformans ATCC 90112. Considering the diameter of the inhibition zone formed on the agar plate, isolates were divided into four sections: section 1 (5-10 mm, slightly-active); section 2 (11-15 mm, moderately-active), section 3 (16-25 mm, highly-active) and section 4 (26-35 mm, ultra-active). It is determined that 3 isolates in section 4 may be a source of novel antibiotic against Cryptococcosis.


Anahtar Kelimeler

Streptomyces Antifungal Activity Cryptococcus neoformans

Kaynakça

  • [1] G. Gebreselema, M. Feleke, S. Samuel, R. Nagappan, “Isolation and characterization of potential antibiotic producing Actinomycetes from water and sediments of Lake Tana Ethiopia”, Asian Pacific Journal of Tropical Biomedicine, vol. 3, pp. 426-35, 2013.
  • [2] J. Berdy, “Bioactive microbial metabolites: a personal view”, The Journal of Antibiotics, vol. 58(1), pp. 1-26, 2005.
  • [3] I. Saadoun, F. Al-Momani, “Steptomycetes from Jordan soils active against Agrobacterium tumefaciens”, Actinomycetes, vol. 8, pp. 29-36, 1997.
  • [4] A.W. Bauer, W.M. Kirby, J.C. Sherris, M. Turk, “Antibiotic susceptibility testing by a standardized single disk method”, American Journal of Clinical Pathology, vol. 45, pp. 493-496, 1966.
  • [5] H.M. Atta, “Production, purification, physico-chemical characteristics and biological activities of antifungal antibiotic produced by Streptomyces antibioticus, AZ-Z710”, American-Eurasian Journal of Scientific Research, vol. 5(1), pp. 39-49, 2010.
  • [6] S. Ramesh, N. Mathivanan, “Screening of marine Actinomycetes isolated from the Bay of Bengal, India for antimicrobial activity and industrial enzymes”, World Journal of Microbiology and Biotechnology, vol. 25, pp. 2103–2111, 2009
  • [7] P.R. Jensen, P.G. Williams, D.C. Oh, L. Zeigler, W. Fenical, “Species-specific secondary metabolite production in marine Actinomycetes of the genus Salinispora”, Applied and Environmental Microbiology, vol. 73, pp. 1146–1152, 2007.
  • [8] A.A. Denizci, “Ege ve Doğu Karadeniz bölgesi topraklarinden izole edilen aktinomisetlerden antibakteriyal antibiyotiklerin aranması ve üretimi üzerine bir araştırma”, Doctoral Tezi, Ege Üniversitesi, Fen Bilimleri Enstitisü, 1996.
  • [9] N. Sahin, A. Ugur, “Investigation of the antimicrobial activity of some Streptomyces isolates”, Turkish Journal of Biology, vol. 27, pp. 79-84, 2003.
  • [10] M. Mohseni, H. Norouzi, J. Hamedi, A. Roohi, “Screening of antibacterial producing actinomycetes from sediments of the Caspian Sea”, International Journal of Molecular and Cellular Medicine, vol. 2(2), pp. 65-71, 2013.
  • [11] C. Coelho, A.L. Bocca, A. Casadevall, “The tools for virulence of Cryptococcus neoformans”, Advances in Applied Microbiology, vol. 87, pp. 1-41, 2014.
  • [12] R. Inci, “Kriptokok infeksiyonları”, Flora, vol. 13(2), pp. 61-71, 2008.
Toplam 12 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Mühendislik
Bölüm Makaleler
Yazarlar

Gorkem Dulger 0000-0002-1506-1549

Başaran Dülger 0000-0002-3184-2652

Yayımlanma Tarihi 25 Nisan 2021
Yayımlandığı Sayı Yıl 2021 Cilt: 9 Sayı: 2

Kaynak Göster

APA Dulger, G., & Dülger, B. (2021). Antifungal Activity of Soil Streptomyces Isolates Against Cryptococcus neoformans. Düzce Üniversitesi Bilim Ve Teknoloji Dergisi, 9(2), 591-595. https://doi.org/10.29130/dubited.862551
AMA Dulger G, Dülger B. Antifungal Activity of Soil Streptomyces Isolates Against Cryptococcus neoformans. DÜBİTED. Nisan 2021;9(2):591-595. doi:10.29130/dubited.862551
Chicago Dulger, Gorkem, ve Başaran Dülger. “Antifungal Activity of Soil Streptomyces Isolates Against Cryptococcus Neoformans”. Düzce Üniversitesi Bilim Ve Teknoloji Dergisi 9, sy. 2 (Nisan 2021): 591-95. https://doi.org/10.29130/dubited.862551.
EndNote Dulger G, Dülger B (01 Nisan 2021) Antifungal Activity of Soil Streptomyces Isolates Against Cryptococcus neoformans. Düzce Üniversitesi Bilim ve Teknoloji Dergisi 9 2 591–595.
IEEE G. Dulger ve B. Dülger, “Antifungal Activity of Soil Streptomyces Isolates Against Cryptococcus neoformans”, DÜBİTED, c. 9, sy. 2, ss. 591–595, 2021, doi: 10.29130/dubited.862551.
ISNAD Dulger, Gorkem - Dülger, Başaran. “Antifungal Activity of Soil Streptomyces Isolates Against Cryptococcus Neoformans”. Düzce Üniversitesi Bilim ve Teknoloji Dergisi 9/2 (Nisan 2021), 591-595. https://doi.org/10.29130/dubited.862551.
JAMA Dulger G, Dülger B. Antifungal Activity of Soil Streptomyces Isolates Against Cryptococcus neoformans. DÜBİTED. 2021;9:591–595.
MLA Dulger, Gorkem ve Başaran Dülger. “Antifungal Activity of Soil Streptomyces Isolates Against Cryptococcus Neoformans”. Düzce Üniversitesi Bilim Ve Teknoloji Dergisi, c. 9, sy. 2, 2021, ss. 591-5, doi:10.29130/dubited.862551.
Vancouver Dulger G, Dülger B. Antifungal Activity of Soil Streptomyces Isolates Against Cryptococcus neoformans. DÜBİTED. 2021;9(2):591-5.
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