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Seroprevalence of Brucellosis in Individuals Engaged in Animal Husbandry in Erzurum Region

Yıl 2023, Cilt: 33 Sayı: 6, 768 - 774, 31.12.2023
https://doi.org/10.54005/geneltip.1334740

Öz

Objective: Brucellosis is one of the most common zoonotic diseases in our country and the world. This study investigated the seroprevalence of brucellosis in individuals who were engaged in animal husbandry in Erzurum region, were over 18 years of age, or did not have any clinical complaints of brucellosis.
Materials and Methods: Our study was conducted on 271 volunteers engaged in livestock farming in 20 districts of Erzurum province between 2020 and 2021. Brucella antibodies were investigated in blood samples using ELISA IgG, ELISA IgM, Rose Bengal, and Standard tube agglutination tests. A questionnaire was administered to the participants to investigate the risk factors of brucellosis.
Results: The mean age of the participants was 43.6 ± 15.7.2 years (min:18, max:90), and there were 137 male (50.6%) and 134 female (49.4%) particpants. Seropositivity was detected with ELISA IgG in 40 of 271 participants (14.8%). Seropositivity was detected in the ELISA IgM test in 2 of these 40 participants (0.7%). Seropositivity was found in 46 participants (17%) in the Rose Bengal and 29 individuals in the STA test. According to the serology results, ELISA IgG positivity was higher in male participants compared to female participants (ratio=1.6). Among pet owners, ELISA IgG seropositivity was found higher than the participants who did not have pets (ratio=1.9). In the evaluation based on educational status, the rate of seropositivity was slightly higher in the participants who were illiterate and had a poor level of education.
Conclusion: In our study, it was determined that the prevalence rate obtained by ELISA IgG was consistent with previous studies conducted in the region. No significant relationship was observed between the serology results of the participants and their gender and educational status. On the other hand, the positivity of ELISA IgG results was higher among the participants who had pets, and there was a significant relationship at the margin.

Teşekkür

We would like to thank those who participated in this research and to Öğr. Gör. Abdulkadir Gülen and Oğuzhan Tunç for their support in the laboratory studies

Kaynakça

  • Dokumacı K. Investi̇gati̇on of brucella anti̇bodi̇es i̇n women wi̇th spontaneous abortus/sti̇llbi̇rths [Master Thesis]: Gaziantep University; 2018.
  • https://hsgm.saglik.gov.tr/tr/zoonotikvektorel-bruselloz/istatistik2021
  • Yüce A, Alp-Çavuş S. Brucellosis in Turkey. A review. Klimik derg 2006;19:87-97.
  • Taşkın HA. Researching Brucella presence by ELISA method in milk and milk products which are consumed in Sivas and Tokat territories and obtaining life time of Brucella melitensis and Brucella abortus bacteria in kefir, [Master Thesis]. Sivas: Cumhuriyet University; 2012.
  • Özcan H. Farmers determine the information levels about brusellozis [Master Thesis]: Kars Kafkas Üniversitesi; 2009.
  • Temiz H, Özcan N, Araç E, Özbek E. D Brucellosis Seroprevalence in Dicle University Medical Faculty Hospital: Rose Bengal Test and Brucella Coombs Test Results. Dicle Tıp Derg 2022;49:660-666.
  • Demirel R, Erdoğan S, Sözen MA. Determination of High Risk Regions of Human Brucellosis in Turkey Using Exploratory Spatial Analysis. Turkiye Klinikleri J Med Sci 2009;29:25-35.
  • Ceylan E, Irmak H, Buzğan T, Karahocagil MK, Evirgen Ö, Sakarya N, et al. V Brucellosis Seropositivity in Human and Animal Population in Some Villages of Van Province. Van Tıp Derg 2003;10:1-5.
  • Dirgen-Çaylak S, Sözen H, Aksözek A, Ekrem Çitil B. Brucella Seropositivity in Veterinarians in Muğla. Klimik Journal/Klimik Derg 2015;28:108-111.
  • Çelebi S. Brusellozun epidemiyolojisi. ANKEM Derg 2003;17:340-343.
  • Uluğ M, Can-Uluğ N. Brusellozlu 78 Olgunun Değerlendirilmesi. Klimik Journal/Klimik Derg 2010;23:89-94.
  • Gürsoy B, Tekin-Koruk S, Sırmatel F, Karaağaç L. Bruselloz: 140 olgunun değerlendirilmesi. Klimik Derg 2008;21:101-104.
  • Sharifi-Mood B, Metanat M, Alavi-Naini R. Correspondence-Screening of the family members of patients with acute Brucellosis in Southeast Iran. Indian J Med Microbiol 2007;25:176-177.
  • Taner Z, Dinç HÖ, Demirci M, Gareayaghi N, Kurt A, Özbey D, et al. Investigation of Brucella Seropositivity in Patients with Suspected Brucellosis: A 4-Year Retrospective Evaluation. Türk Mikrobiyoloji Cem Derg 2019;49:212-218.
  • Ataman-Hatipoğlu Ç, Kınıklı S, Tülek N, Koruk Tekin S, Arslan S, Tuncer Ertem G. Evaluation of epidemiological data of 202 patients with brucellosis. Klimik Derg 2005;18:94-98.
  • Gültekin E. Comparison of Various Serological Methods Used for Laboratory Diagnosis of Brucellosis [Master Thesis]. Erzurum: Atatürk University; 2012.
  • Sözen TH. Bruselloz. In: Topçu AV, Söyletir G, Doğanay M, editors. İnfeksiyon Hastalıkları ve Mikrobiyolojisi. 1. 3. Baskı ed. İstanbul: Nobel Tıp Kitabevleri; 2002. p. 636-642.
  • Özer S, Oltan N, Gençer S. Bruselloz: An evaluation of 33 cases. Klimik Derg 1998;11:82-84.
  • Taşova Y, Saltoğlu N, Yılmaz G, İnal AS, Aksu HS. Bruselloz: An evaluation of clinical, laboratory and treatment features of 238 adult cases. İnfeksiyon Dergisi. 1998;12:307-312.
  • Pappas G, Akritidis N, Bosilkovski M, Tsianos E. Brucellosis. N Engl J Med. 2005;352:2325-2336.
  • Çetinkaya Z. Klinik Mikrobiyoloji, Başustaoğlu A, Kubar A, Yıldıran ŞT, Tanyüksel M, (translation editors) Francisella ve Brucella. In: Murray PR BE, Pfaller MA, Landry ML, Jorgensen JH, editor. Manual of Clinical Microbiology,. 9. Ankara: Atlas Kitapçılık; 2009. p. 815-834.
  • Alışkan HE, Çolakoğlu Ş, Turunç T, Demiroğlu YZ, Yazıcı Güvercin AC, Arslan AH. Evaluation of diagnostic value of Brucellacapt test in brucellosis. Mikrobiyol Bul 2007;41:591-595.
  • Arabacı F, Oldacay M. Evaluation of serological diagnostic tests for human Brucellosis in an endemic area. Journal of Microbiology and Infectious Diseases. 2012;2:50-56.
  • Güzelant A, Kurtoğlu MG, Kaya M, Keşli R, Terzi Y, Baysal B. Comparison of the Brucellacapt with other serological tests used for the diagnosis of brucellosis. Selçuk Tıp Derg 2009;25:125-131.
  • Young EJ. Brucella species. In: Mandell G, Bennett J, Dolin R, editors. Principles and Practice of Infectious Diseases. 2. 2 nd ed. New York: Churchill Livingstone; 2000. p. 2386-2392.
  • Aydın L. K The investigation of brucellosis by serological tests (rose bengal plate, modified rose bengal plate, serum agglutination, rivanol agglutination, 2-mercaphtoethanol aglutination and ELISA) in patients with complaints of fever and joint pain in health centers in Konya [Master Thesis]. Konya: Selçuk University; 2009.
  • Araj GF. Update on laboratory diagnosis of human brucellosis. Int J Antimicrob Agents. 2010;36:12-17.
  • Uysal B. Comparison of diagnostic tests in brucellosis [Master Thesis]: Kayseri Erciyes University; 2012.
  • Foster G, Osterman BS, Godfroid J, Jacques I, Cloeckaert A. Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts. Int J Syst Evol Microbiol 2007;57:2688-2693.
  • Gómez MC, Nieto JA, Rosa C, Geijo P, Escribano MA, Munoz A, et al. Evaluation of seven tests for diagnosis of human brucellosis in an area where the disease is endemic. Clin Vaccine Immunol 2008;15:1031-1033.
  • Welch RJ, Litwin CM. A comparison of Brucella IgG and IgM ELISA assays with agglutination methodology. J Clin Lab Anal 2010;24:160-162.
  • Kalem F, Ergün AG, Durmaz S, Doğan M, Ertuğrul Ö, Gündem S. Comparison of a new and rapid method: Brucella coombs gel test with other diagnostic tests. J Clin Lab Anal 2016;30:756-759.

Erzurum Yöresinde Hayvancılıkla Uğraşan Kişilerde Brusella Seroprevalansı

Yıl 2023, Cilt: 33 Sayı: 6, 768 - 774, 31.12.2023
https://doi.org/10.54005/geneltip.1334740

Öz

Amaç: Bruselloz dünyada ve ülkemizde en sık görülen zoonotik hastalıklardan birisidir. Bu araştırma Erzurum bölgesinde hayvancılıkla uğraşan ve bruselloz açısından herhangi bir klinik şikayeti olmayan 18 yaş üstü kişilerde brusella seroprevalansını araştırma amacıyla yapılmıştır.
Yöntem: Çalışmamız 2020-2021 yıllarında Erzurum ilinin 20 ilçesinde hayvancılık faaliyetlerinde bulunan 271 gönüllü kişi üzerinde yürütüldü. Alınan kan örneklerinde Brusella antikorları; ELISA IgG, ELISA IgM, Rose Bengal ve Standart Tüp aglütinasyon testleri kullanılarak araştırıldı. Bruselloz açısından risk faktörlerini araştırmak amacıyla katılımcılara anket formu dolduruldu.
Bulgular: Katılımcıların yaş ortalaması 43.6±15.7.2 yıl (min:18, maks:90) ve erkekler 137(%50.6), kadınlar ise 134 (%49.4) kişi idi. Çalışmamıza katılan 271 kişinin 40’ında ELISA IgG ile seropozitiflik saptanmıştır (%14.8). Bu 40 kişiden iki kişide aynı zamanda ELISA IgM seropozitifliğine rastlanmıştır (%0.7). Rose Bengal ile 46 kişide (%17), STA yöntemi ile 29 kişide seropozitiflik bulunmuştur. Seroloji sonuçlarına göre erkeklerde, kadınlara göre ELİSA IgG pozitifliği yüksek bulunmuştur (oran=1,6). Evcil hayvan besleyenlerde, evcil hayvanı olmayanlara göre ELISA IgG seropozitifiği daha yüksek bulundu (oran=1,9). Eğitim durumuna göre yapılan değerlendirmede okur yazar olmayan ve eğitim seviyesi düşük olan katılımcılarda seropozitiflik oranı biraz daha yüksek bulunmuştur.
Sonuç: Çalışmamızda ELISA IgG ile belirlenen prevalans oranı bölgemizde daha önce yapılmış çalışmalarla uyumlu bulundu. Çalışmamızda cinsiyet ve öğrenim durumu ile katılımcıların seroloji sonuçları arasında anlamlı bir ilişki gözlenmedi. Fakat evcil hayvana sahip katılımcılarda ELISA IgG sonuçlarının pozitifliği daha yüksek ve sınırda anlamlı ilişki söz konusuydu.

Kaynakça

  • Dokumacı K. Investi̇gati̇on of brucella anti̇bodi̇es i̇n women wi̇th spontaneous abortus/sti̇llbi̇rths [Master Thesis]: Gaziantep University; 2018.
  • https://hsgm.saglik.gov.tr/tr/zoonotikvektorel-bruselloz/istatistik2021
  • Yüce A, Alp-Çavuş S. Brucellosis in Turkey. A review. Klimik derg 2006;19:87-97.
  • Taşkın HA. Researching Brucella presence by ELISA method in milk and milk products which are consumed in Sivas and Tokat territories and obtaining life time of Brucella melitensis and Brucella abortus bacteria in kefir, [Master Thesis]. Sivas: Cumhuriyet University; 2012.
  • Özcan H. Farmers determine the information levels about brusellozis [Master Thesis]: Kars Kafkas Üniversitesi; 2009.
  • Temiz H, Özcan N, Araç E, Özbek E. D Brucellosis Seroprevalence in Dicle University Medical Faculty Hospital: Rose Bengal Test and Brucella Coombs Test Results. Dicle Tıp Derg 2022;49:660-666.
  • Demirel R, Erdoğan S, Sözen MA. Determination of High Risk Regions of Human Brucellosis in Turkey Using Exploratory Spatial Analysis. Turkiye Klinikleri J Med Sci 2009;29:25-35.
  • Ceylan E, Irmak H, Buzğan T, Karahocagil MK, Evirgen Ö, Sakarya N, et al. V Brucellosis Seropositivity in Human and Animal Population in Some Villages of Van Province. Van Tıp Derg 2003;10:1-5.
  • Dirgen-Çaylak S, Sözen H, Aksözek A, Ekrem Çitil B. Brucella Seropositivity in Veterinarians in Muğla. Klimik Journal/Klimik Derg 2015;28:108-111.
  • Çelebi S. Brusellozun epidemiyolojisi. ANKEM Derg 2003;17:340-343.
  • Uluğ M, Can-Uluğ N. Brusellozlu 78 Olgunun Değerlendirilmesi. Klimik Journal/Klimik Derg 2010;23:89-94.
  • Gürsoy B, Tekin-Koruk S, Sırmatel F, Karaağaç L. Bruselloz: 140 olgunun değerlendirilmesi. Klimik Derg 2008;21:101-104.
  • Sharifi-Mood B, Metanat M, Alavi-Naini R. Correspondence-Screening of the family members of patients with acute Brucellosis in Southeast Iran. Indian J Med Microbiol 2007;25:176-177.
  • Taner Z, Dinç HÖ, Demirci M, Gareayaghi N, Kurt A, Özbey D, et al. Investigation of Brucella Seropositivity in Patients with Suspected Brucellosis: A 4-Year Retrospective Evaluation. Türk Mikrobiyoloji Cem Derg 2019;49:212-218.
  • Ataman-Hatipoğlu Ç, Kınıklı S, Tülek N, Koruk Tekin S, Arslan S, Tuncer Ertem G. Evaluation of epidemiological data of 202 patients with brucellosis. Klimik Derg 2005;18:94-98.
  • Gültekin E. Comparison of Various Serological Methods Used for Laboratory Diagnosis of Brucellosis [Master Thesis]. Erzurum: Atatürk University; 2012.
  • Sözen TH. Bruselloz. In: Topçu AV, Söyletir G, Doğanay M, editors. İnfeksiyon Hastalıkları ve Mikrobiyolojisi. 1. 3. Baskı ed. İstanbul: Nobel Tıp Kitabevleri; 2002. p. 636-642.
  • Özer S, Oltan N, Gençer S. Bruselloz: An evaluation of 33 cases. Klimik Derg 1998;11:82-84.
  • Taşova Y, Saltoğlu N, Yılmaz G, İnal AS, Aksu HS. Bruselloz: An evaluation of clinical, laboratory and treatment features of 238 adult cases. İnfeksiyon Dergisi. 1998;12:307-312.
  • Pappas G, Akritidis N, Bosilkovski M, Tsianos E. Brucellosis. N Engl J Med. 2005;352:2325-2336.
  • Çetinkaya Z. Klinik Mikrobiyoloji, Başustaoğlu A, Kubar A, Yıldıran ŞT, Tanyüksel M, (translation editors) Francisella ve Brucella. In: Murray PR BE, Pfaller MA, Landry ML, Jorgensen JH, editor. Manual of Clinical Microbiology,. 9. Ankara: Atlas Kitapçılık; 2009. p. 815-834.
  • Alışkan HE, Çolakoğlu Ş, Turunç T, Demiroğlu YZ, Yazıcı Güvercin AC, Arslan AH. Evaluation of diagnostic value of Brucellacapt test in brucellosis. Mikrobiyol Bul 2007;41:591-595.
  • Arabacı F, Oldacay M. Evaluation of serological diagnostic tests for human Brucellosis in an endemic area. Journal of Microbiology and Infectious Diseases. 2012;2:50-56.
  • Güzelant A, Kurtoğlu MG, Kaya M, Keşli R, Terzi Y, Baysal B. Comparison of the Brucellacapt with other serological tests used for the diagnosis of brucellosis. Selçuk Tıp Derg 2009;25:125-131.
  • Young EJ. Brucella species. In: Mandell G, Bennett J, Dolin R, editors. Principles and Practice of Infectious Diseases. 2. 2 nd ed. New York: Churchill Livingstone; 2000. p. 2386-2392.
  • Aydın L. K The investigation of brucellosis by serological tests (rose bengal plate, modified rose bengal plate, serum agglutination, rivanol agglutination, 2-mercaphtoethanol aglutination and ELISA) in patients with complaints of fever and joint pain in health centers in Konya [Master Thesis]. Konya: Selçuk University; 2009.
  • Araj GF. Update on laboratory diagnosis of human brucellosis. Int J Antimicrob Agents. 2010;36:12-17.
  • Uysal B. Comparison of diagnostic tests in brucellosis [Master Thesis]: Kayseri Erciyes University; 2012.
  • Foster G, Osterman BS, Godfroid J, Jacques I, Cloeckaert A. Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts. Int J Syst Evol Microbiol 2007;57:2688-2693.
  • Gómez MC, Nieto JA, Rosa C, Geijo P, Escribano MA, Munoz A, et al. Evaluation of seven tests for diagnosis of human brucellosis in an area where the disease is endemic. Clin Vaccine Immunol 2008;15:1031-1033.
  • Welch RJ, Litwin CM. A comparison of Brucella IgG and IgM ELISA assays with agglutination methodology. J Clin Lab Anal 2010;24:160-162.
  • Kalem F, Ergün AG, Durmaz S, Doğan M, Ertuğrul Ö, Gündem S. Comparison of a new and rapid method: Brucella coombs gel test with other diagnostic tests. J Clin Lab Anal 2016;30:756-759.
Toplam 32 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Klinik Mikrobiyoloji
Bölüm Original Article
Yazarlar

Mahmut Ucar 0000-0003-2740-6476

Ahmet Yılmaz 0000-0002-2350-1516

Selahattin Çelebi 0000-0003-0188-9668

Sinan Yılmaz 0000-0001-7784-3274

Erken Görünüm Tarihi 29 Aralık 2023
Yayımlanma Tarihi 31 Aralık 2023
Gönderilme Tarihi 30 Temmuz 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 33 Sayı: 6

Kaynak Göster

Vancouver Ucar M, Yılmaz A, Çelebi S, Yılmaz S. Seroprevalence of Brucellosis in Individuals Engaged in Animal Husbandry in Erzurum Region. Genel Tıp Derg. 2023;33(6):768-74.