Year 2016, Volume 1 , Issue 2, Pages 43 - 47 2016-08-30

Çeşitli klinik örneklerden izole edilen stafilokok türlerinde vankomisin direncinin araştırılması
Investigation of vancomycin resistance in staphylococcus strains isolated from various clinical samples

Satı Zeynep Tekin [1] , Sebahat Aksaray [2] , Fatma Bozkurt [3] , Gülden Albayrak [4] , Jale Taşcıoğlu [5]


Giriş: Son yıllarda Staphylococcus aureus’ta sınırlı sayıda vankomisin direncinin yanında vankomisine azalmış duyarlılıktan da söz edilmektedir. Çalışmamızda çeşitli klinik örneklerden izole edilen Stafilokokus suşlarında vankomisin duyarlılık testi çalışılması amaçlanmıştır.

Yöntem: Metisiline dirençli olan toplam 164 Staphylococcus aureus ve koagülaz negatif stafilokok suşunda vankomisin agar tarama ve E-test yöntemiyle vankomisin duyarlılığı çalışılmış ve sonuçlar Clinical and Laboratory Standart Institute ve Europian Committee on Antimicrobial Susceptibility Testing kriterlerince irdelenmiştir.

Bulgular: Hasta örneklerinden izole edilen 164 (93 Metisilin Dirençli Staphylococcus aureus ve 71 Metisilin Dirençli Koagulaz Negatif Stafilokok) stafilokok suşunun E-Test ile değerlendirmesinde Clinical and Laboratory Standart Institute'ye göre vankomisine hassasiyeti azalmış ya da dirençli suşa rastlanmadı. Europian Committee on Antimicrobial Susceptibility Testing'e göre Brain Heart infüzyon agarda üreyen 6 Metisilin Dirençli Staphylococcus aureus suşunun 3'ü, 7 Metisilin Dirençli Koagulaz Negatif Stafilokok suşunun 1'i Vankomisin'e dirençli tespit edilmiştir.

Sonuç: Minimum İnhibitör Konsantrasyon değerindeki küçük değişiklikler bile klinik olarak anlamlı olabileceğinden, Vankomisin Minimum İnhibitör Konsantrasyon değeri doğru tespit edilmelidir.

Introduction: Besides the few investigation demonstrating the vancomycin resistance towards Staphylococcus aureus in recent years, it has been also reported a decreased sensitivity of these bacteria to vancomycin. In the present study, it was aimed to evaluate sensitivity test in staphylococcus strains isolated from various clinical samples.

Methods: A total of 164 staphylococcus strains consisting of Methicillin Resistant Staphylococcus aureus (n=93), and Methicillin Resistant Coagulase Negative Staphylococcus (n=71) were included in this study. The vancomycin agar screening and E-test methods were used to assess vancomycin sensitivity, and results were interpreted to Clinical and Laboratory Standard Institute and European Committee on Antimicrobial Susceptibility Testing criteria.

Results: After assessing the strains by using E-Test, neither decreased sensitivity, nor resistance to vancomycin was determined according to Clinical and Laboratory Standard Institute. However, 3 of 6 Methicillin Resistant Staphylococcus aureus, and 1 of 7 Methicillin Resistant Coagulase Negative Staphylococcus reproduced in Brain Heart Infusion Agar were found to be resistant to vancomycin according to European Committee on Antimicrobial Susceptibility Testing.

Conclusion: Because even small changes can be clinically significant, Minimum İnhibition Concentration value of vancomycin should be correctly determined.

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Primary Language en
Subjects Health Care Sciences and Services
Published Date Aug 2016
Journal Section Original Research
Authors

Author: Satı Zeynep Tekin
Institution: Çanakkale Onsekiz Mart Üniversitesi, Tıbbi Mikrobiyoloji Ana Bilim Dalı, Çanakkale
Country: Turkey


Author: Sebahat Aksaray
Institution: Haydarpaşa Numune Eğitim ve Araştırma Hastanesi, Klinik Mikrobiyoloji Laboratuvarı, İstanbul
Country: Turkey


Author: Fatma Bozkurt
Institution: Dr. Lütfi Kırdar Kartal Eğitim ve Araştırma Hastanesi, Klinik Mikrobiyoloji Laboratuvarı, İstanbul, Türkiye
Country: Turkey


Author: Gülden Albayrak
Institution: Çarşamba Devlet Hastanesi, Klinik Mikrobiyoloji Laboratuvarı, Samsun
Country: Turkey


Author: Jale Taşcıoğlu
Institution: Haydarpaşa Numune Eğitim ve Araştırma Hastanesi, Klinik Mikrobiyoloji Laboratuvarı, İstanbul
Country: Turkey


Dates

Publication Date : August 30, 2016

Bibtex @research article { fppc256691, journal = {Family Practice and Palliative Care}, issn = {2458-8865}, eissn = {2459-1505}, address = {}, publisher = {SERNEV}, year = {2016}, volume = {1}, pages = {43 - 47}, doi = {10.22391/920.256691}, title = {Investigation of vancomycin resistance in staphylococcus strains isolated from various clinical samples}, key = {cite}, author = {Tekin, Satı Zeynep and Aksaray, Sebahat and Bozkurt, Fatma and Albayrak, Gülden and Taşcıoğlu, Jale} }
APA Tekin, S , Aksaray, S , Bozkurt, F , Albayrak, G , Taşcıoğlu, J . (2016). Investigation of vancomycin resistance in staphylococcus strains isolated from various clinical samples. Family Practice and Palliative Care , 1 (2) , 43-47 . DOI: 10.22391/920.256691
MLA Tekin, S , Aksaray, S , Bozkurt, F , Albayrak, G , Taşcıoğlu, J . "Investigation of vancomycin resistance in staphylococcus strains isolated from various clinical samples". Family Practice and Palliative Care 1 (2016 ): 43-47 <http://www.fppc.com.tr/en/issue/24207/256691>
Chicago Tekin, S , Aksaray, S , Bozkurt, F , Albayrak, G , Taşcıoğlu, J . "Investigation of vancomycin resistance in staphylococcus strains isolated from various clinical samples". Family Practice and Palliative Care 1 (2016 ): 43-47
RIS TY - JOUR T1 - Investigation of vancomycin resistance in staphylococcus strains isolated from various clinical samples AU - Satı Zeynep Tekin , Sebahat Aksaray , Fatma Bozkurt , Gülden Albayrak , Jale Taşcıoğlu Y1 - 2016 PY - 2016 N1 - doi: 10.22391/920.256691 DO - 10.22391/920.256691 T2 - Family Practice and Palliative Care JF - Journal JO - JOR SP - 43 EP - 47 VL - 1 IS - 2 SN - 2458-8865-2459-1505 M3 - doi: 10.22391/920.256691 UR - https://doi.org/10.22391/920.256691 Y2 - 2016 ER -
EndNote %0 Family Practice and Palliative Care Investigation of vancomycin resistance in staphylococcus strains isolated from various clinical samples %A Satı Zeynep Tekin , Sebahat Aksaray , Fatma Bozkurt , Gülden Albayrak , Jale Taşcıoğlu %T Investigation of vancomycin resistance in staphylococcus strains isolated from various clinical samples %D 2016 %J Family Practice and Palliative Care %P 2458-8865-2459-1505 %V 1 %N 2 %R doi: 10.22391/920.256691 %U 10.22391/920.256691
ISNAD Tekin, Satı Zeynep , Aksaray, Sebahat , Bozkurt, Fatma , Albayrak, Gülden , Taşcıoğlu, Jale . "Investigation of vancomycin resistance in staphylococcus strains isolated from various clinical samples". Family Practice and Palliative Care 1 / 2 (August 2016): 43-47 . https://doi.org/10.22391/920.256691
AMA Tekin S , Aksaray S , Bozkurt F , Albayrak G , Taşcıoğlu J . Investigation of vancomycin resistance in staphylococcus strains isolated from various clinical samples. Fam Pract Palliat Care. 2016; 1(2): 43-47.
Vancouver Tekin S , Aksaray S , Bozkurt F , Albayrak G , Taşcıoğlu J . Investigation of vancomycin resistance in staphylococcus strains isolated from various clinical samples. Family Practice and Palliative Care. 2016; 1(2): 47-43.