Year 2019, Volume 4 , Issue 3, Pages 79 - 84 2019-12-31

Tavşan modelinde ekstremite iskemi-reperfüzyon hasarına ozon tedavisinin etkisi
The effects of ozone therapy on extremity ischemia-reperfusion injury in a rabbit model

Burak KAYMAZ [1] , Ganime COBAN [2] , Ferdi GOKSEL [3] , Huseyin TOMAN [4] , Umut Hatay GOLGE [5] , Erkam KOMURCU [6]


Giriş: İskemi; organı veya dokuyu perfüze eden kan akımındaki yetersizliğe bağlı olarak gelişen geri dönüşümlü veya dönüşümsüz hücre/doku zedelenmesidir. İskemi sonrasında hücrelerde pek çok metabolik ve yapısal değişiklikler oluşmaktadır. Ozon (O3) çeşitli hastalıkları tedavisi için antioksidan olarak terapötik amaçlı kullanılmaktadır. Bu çalışmada, ozon tedavisinin ekstremite iskemi-reperfüzyon hasarı üzerine olan etkisinin incelenmesi amaçlanmıştır.

Yöntem: 24 adet New Zealand White (NZW) erişkin erkek tavşan rastgele 3 gruba ayrıldı.Grup1: Kontrol grubu (n:8) Grup II: İskemi grubu (n:8) ve Grup III: Ozon grubu (n:8). Deneklerin sağ bacaklarına femoral arter oklüzyonu uygulanıp Grup II ve Grup III de iskemi-reperfüzyon hasarı uygulandı. Kontrol grubunda disseksiyon yapılıp oklüzyon uygulanmadı. Deneklerin kan değerleri ve sakrifikasyon sonrası kas dokusu histopatolojik değerlendirmesi yapıldı.

Bulgular: İşlem sonrası iskemi grubuyla ozon tedavisi uygulanan gruplar karşılaştırıldığında; TAS (total antioksidan status) düzeyinin ozon tedavisi uygulanan grupta anlamlı düzeyde daha yüksek (p=0,036) ve MDA (malondialdehit) düzeyinin de ozon tedavisi uygulanan grupta daha düşük olduğu, ancak istatistiksel olarak anlamlılığa (p=0,093) ulaşmadığı görüldü. Histopatolojik açıdan da ozon grubu ile kontrol grubunun benzer olduğu görüldü.

Sonuç: Ozon tedavisi güçlü anti-inflamatuvar ve antioksidan etkinliği ile ekstermite iskemi-reperfüzyon (IR) hasarını azaltmada alternatif bir tedavi seçeneği olabilir.

Introduction: Ischemia is reversible or irreversible cell/tissue damage that is secondary to insufficient blood flow to tissues or organs. Ischemia causes many metabolic and structural changes at the cellular level. Ozone therapy is often used as an antioxidant remedy. This study aimed to investigate the effects of ozone therapy on extremity ischemia-reperfusion (IR) injury.

Methods: Twenty-four New Zealand White (NZW) rabbits were randomly allocated into three groups. Each group consisted of eight rabbits. Group I was the control group, Group II was the ischemia group, and Group III, the ozone group.  Femoral arteries of the right legs were dissected, and femoral arterial occlusion was performed in Group II and III.  Hematological and histopathological evaluation was performed in all groups.

Results: The levels of total antioxidant status (TAS) after the surgical procedure was higher in the ozone group compared to the ischemia group (p=0.036). In addition, the levels of malondialdehyde (MDA) after the surgical procedure were lower in the ozone group compared to the ischemia group but not statistically significant (p=0.093). The control and ozone groups were histopathologically similar.

Conclusions: Ozone therapy may be used as an alternative treatment modality with its anti-inflammatory and antioxidant effects for the treatment of extremity IR injury.

  • 1. Turoczi Z, Aranyi P, Lukats A. Muscle Fiber Viability, A novel method for the fast detection of ischemic muscle injury in Rats PLoS One 2014;9(1):e84783 https://doi.org/10.1371/journal.pone.0084783
  • 2. Chen H1, Xing B, Liu X, Zhan B, Zhan B, Zhou J, et al. Ozone oxidative preconditioning inhibits inflammation and apoptosis in a rat model of renal ischemia/reperfusion injury. Eur J Pharmacol 2008;581(3):306-14. https://doi.org/10.1016/j.ejphar.2007.11.050
  • 3. Fernández I, González Núñez L, Calunga Fernández JL, Rodríguez Salgueiro S, Santos Febles E. Ozone postconditioning in renal ischaemia-reperfusion model. Functional and morphological evidences. Nefrologia 2011;31(4):464-70. https://doi.org/10.3265/Nefrologia.pre2011.May.10873
  • 4. Caliskan B, Guven A, Ozler M, Cayci T, Ozcan A, Bedir O, et al. Ozone therapy prevents renal inflammation and fibrosis in a rat model of acute pyelonephritis. Scand J Clin Lab Invest 2011;71(6):473-80. https://doi.org/10.3109/00365513.2011.587022
  • 5. Kumada M, Kihara S, Sumitsuji S, Kawamoto T, Matsumoto S, Ouchi N, et al. Association of hypoadiponectinemia with coronary artery disease in men. Arterioscler Thromb Vasc Biol 2003;23(1):85-9. https:// doi.org/10.1161/01.atv.0000048856.22331.50
  • 6. Ouchi N, Ohishi M, Kihara S, Funahashi T, NakamuraT, Nagaretani H, et al. Association of hypoadiponectinemia with impaired vasoreactivity. Hypertension 2003;42:231. https://doi.org/10.1161/01.HYP.0000083488.67550.B8
  • 7. Bulger EM, Cuschieri J, Warner K, Maier R. Hypertonic resuscitation modulates the inflammatory response in patients with traumatic hemorrhagic shock. Ann Surg 2007;245:635. https://doi.org/10.1097/01.sla.0000251367.44890.ae
  • 8. Kupiec-Weglinski JW, Busuttil RW. Ischemia and reperfusion injury in liver transplantation. Transpl Proc 2005;37:1653. https://doi.org/10.1016/j.transproceed.2005.03.134
  • 9. Granger DN, Kvietys PR, Perry MA. Leukocyte-endothelial cell adhesion induced by ischemia and reperfusion. Can J Physiol Pharmacol 1993;71:67–75. https://doi.org/10.1139/y93-011
  • 10. Kirschner RE, Fyfe BS, Hoffman LA, Chiao JJ, Davis JM, Fantini GA. Ischemia-reperfusion infury in myocutaneous flaps: role of leukocytes and leukotrienes. Plast Reconstr Surg 1997;99:1485–1493. https://www.ncbi.nlm.nih.gov/pubmed/9145114
  • 11. Cangemi R, Angelico F, Loffredo L, Del Ben M, Pignatelli P, Martini A, et al. Oxidative stress mediated arterial dysfunction in patients with metabolic syndrome: effect of ascorbic acid. Free Radic Biol Med 2007; 43:853. https://doi.org/10.1016/j.freeradbiomed.2007.06.002
  • 12. Webster NR, Nunn JF. Molecular structure of free radicals and their importance in biological reactions. Br J Anaesth 1988;60:98. https://doi.org/10.1093/bja/60.1.98
  • 13. Parabucki AB, Bozic ID, Bjelobaba IM, LavrnjaI, Brykc P, Jovanovic T, et al. Hyperbaric oxygenation alters temporal expression pattern of superoxide dismutase 2 after cortical stab injury in rats. Croat Med J 2012;53:586. https://doi.org/10.3325/cmj.2012.53.586
  • 14. Tural E, Uslu S, Ilgaz Aydinlar E, Irban A, Ince U, Orakdogen M, et al. Effects of ozone on spinal cord recovery via Wnt/ β-catenin pathway following spinal cord injury in rats. Turk Neurosurg 2017;27(6):946-54. https://doi.org/10.5137/1019-5149.JTN.17508-16.1
  • 15. Muto M, Giurazza F, Silva RP, Guarnieri G. Rational approach, technique and selection criteria treating lumbar disk herniations by oxygen-ozone therapy. Interv Neuroradiol 2016;22(6):736-40. https://doi.org/10.1177/1591019916659266
  • 16. Ergun Tasdoven G, Derin AT, Yaprak N, Ozcaglar HU. The place of hyperbaric oxygen therapy and ozone therapy in sudden hearing loss. Braz J Otorhinolaryngol 2017;83(4):457-63. https://doi.org/10.1016/j.bjorl.2016.06.002
  • 17. León Fernández OS, Viebahn-Haensler R, Cabreja GL, Espinosa IS, Matos YH, Roche LD, et al. Medical ozone increases methotrexate clinical response and improves cellular redox balance in patients with rheumatoid arthritis. Eur J Pharmacol 2016;789:313-8. https://doi.org/10.1016/j.ejphar.2016.07.031
Primary Language en
Subjects Surgery
Published Date Dec
Journal Section Original Research
Authors

Orcid: 0000-0001-6207-8063
Author: Burak KAYMAZ (Primary Author)
Institution: Department of Orthopedics and Traumatology, Canakkale Onsekiz Mart University, Canakkale
Country: Turkey


Orcid: 0000-0002-5779-6797
Author: Ganime COBAN
Institution: Department of Pathology, Bezmialem Vakif University, Istanbul
Country: Turkey


Orcid: 0000-0002-2672-2904
Author: Ferdi GOKSEL
Institution: Department of Orthopedics and Traumatology, Eregli Medical Park Hospital, Zonguldak
Country: Turkey


Orcid: 0000-0003-2708-4163
Author: Huseyin TOMAN
Institution: Department of Anesthesiology and Reanimation, Medicana International Hospital, Istanbul
Country: Turkey


Orcid: 0000-0001-7384-6144
Author: Umut Hatay GOLGE
Institution: Specialist of Orthopedics and Traumatology
Country: Turkey


Orcid: 0000-0003-3358-0358
Author: Erkam KOMURCU
Institution: Department of Orthopedics and Traumatology, Medical Park Gaziosmanpasa Hospital, Istanbul
Country: Turkey


Supporting Institution This work was supported by Research Fund of the Çanakkale Onsekiz Mart University
Project Number 2015-545
Dates

Application Date : July 26, 2019
Acceptance Date : October 24, 2019
Publication Date : December 31, 2019

Bibtex @research article { fppc596783, journal = {Family Practice and Palliative Care}, issn = {2458-8865}, eissn = {2459-1505}, address = {}, publisher = {SERNEV}, year = {2019}, volume = {4}, pages = {79 - 84}, doi = {10.22391/fppc.596783}, title = {The effects of ozone therapy on extremity ischemia-reperfusion injury in a rabbit model}, key = {cite}, author = {KAYMAZ, Burak and COBAN, Ganime and GOKSEL, Ferdi and TOMAN, Huseyin and GOLGE, Umut Hatay and KOMURCU, Erkam} }
APA KAYMAZ, B , COBAN, G , GOKSEL, F , TOMAN, H , GOLGE, U , KOMURCU, E . (2019). The effects of ozone therapy on extremity ischemia-reperfusion injury in a rabbit model. Family Practice and Palliative Care , 4 (3) , 79-84 . DOI: 10.22391/fppc.596783
MLA KAYMAZ, B , COBAN, G , GOKSEL, F , TOMAN, H , GOLGE, U , KOMURCU, E . "The effects of ozone therapy on extremity ischemia-reperfusion injury in a rabbit model". Family Practice and Palliative Care 4 (2019 ): 79-84 <http://www.fppc.com.tr/en/issue/50274/596783>
Chicago KAYMAZ, B , COBAN, G , GOKSEL, F , TOMAN, H , GOLGE, U , KOMURCU, E . "The effects of ozone therapy on extremity ischemia-reperfusion injury in a rabbit model". Family Practice and Palliative Care 4 (2019 ): 79-84
RIS TY - JOUR T1 - The effects of ozone therapy on extremity ischemia-reperfusion injury in a rabbit model AU - Burak KAYMAZ , Ganime COBAN , Ferdi GOKSEL , Huseyin TOMAN , Umut Hatay GOLGE , Erkam KOMURCU Y1 - 2019 PY - 2019 N1 - doi: 10.22391/fppc.596783 DO - 10.22391/fppc.596783 T2 - Family Practice and Palliative Care JF - Journal JO - JOR SP - 79 EP - 84 VL - 4 IS - 3 SN - 2458-8865-2459-1505 M3 - doi: 10.22391/fppc.596783 UR - https://doi.org/10.22391/fppc.596783 Y2 - 2019 ER -
EndNote %0 Family Practice and Palliative Care The effects of ozone therapy on extremity ischemia-reperfusion injury in a rabbit model %A Burak KAYMAZ , Ganime COBAN , Ferdi GOKSEL , Huseyin TOMAN , Umut Hatay GOLGE , Erkam KOMURCU %T The effects of ozone therapy on extremity ischemia-reperfusion injury in a rabbit model %D 2019 %J Family Practice and Palliative Care %P 2458-8865-2459-1505 %V 4 %N 3 %R doi: 10.22391/fppc.596783 %U 10.22391/fppc.596783
ISNAD KAYMAZ, Burak , COBAN, Ganime , GOKSEL, Ferdi , TOMAN, Huseyin , GOLGE, Umut Hatay , KOMURCU, Erkam . "The effects of ozone therapy on extremity ischemia-reperfusion injury in a rabbit model". Family Practice and Palliative Care 4 / 3 (December 2020): 79-84 . https://doi.org/10.22391/fppc.596783
AMA KAYMAZ B , COBAN G , GOKSEL F , TOMAN H , GOLGE U , KOMURCU E . The effects of ozone therapy on extremity ischemia-reperfusion injury in a rabbit model. Fam Pract Palliat Care. 2019; 4(3): 79-84.
Vancouver KAYMAZ B , COBAN G , GOKSEL F , TOMAN H , GOLGE U , KOMURCU E . The effects of ozone therapy on extremity ischemia-reperfusion injury in a rabbit model. Family Practice and Palliative Care. 2019; 4(3): 84-79.