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Araştırma Makalesi
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Tavşan modelinde ekstremite iskemi-reperfüzyon hasarına ozon tedavisinin etkisi

Yıl 2019, Cilt: 4 Sayı: 3, 79 - 84, 31.12.2019

Burak Kaymaz , Ganime Coban , Ferdi Goksel , Huseyin Toman , Umut Hatay Golge , Erkam Komurcu

https://doi.org/10.22391/fppc.596783

Öz



Giriş: İskemi; organı veya dokuyu perfüze eden kan
akımındaki yetersizliğe bağlı olarak gelişen geri dönüşümlü veya dönüşümsüz
hücre/doku zedelenmesidir. İskemi sonrasında hücrelerde pek çok metabolik ve
yapısal değişiklikler oluşmaktadır. Ozon (O3) çeşitli hastalıkları tedavisi
için antioksidan olarak terapötik amaçlı kullanılmaktadır. Bu çalışmada, ozon
tedavisinin ekstremite iskemi-reperfüzyon hasarı üzerine olan etkisinin
incelenmesi amaçlanmıştır.



Yöntem: 24
adet New Zealand White (NZW) erişkin erkek tavşan rastgele 3 gruba
ayrıldı.Grup1: Kontrol grubu (n:8) Grup II: İskemi grubu (n:8) ve Grup III:
Ozon grubu (n:8). Deneklerin sağ bacaklarına femoral arter oklüzyonu uygulanıp
Grup II ve Grup III de iskemi-reperfüzyon hasarı uygulandı. Kontrol grubunda
disseksiyon yapılıp oklüzyon uygulanmadı. Deneklerin kan değerleri ve
sakrifikasyon sonrası kas dokusu histopatolojik değerlendirmesi yapıldı.



Bulgular: İşlem
sonrası iskemi grubuyla ozon tedavisi uygulanan gruplar karşılaştırıldığında;
TAS (total antioksidan status) düzeyinin ozon tedavisi uygulanan grupta anlamlı
düzeyde daha yüksek (p=0,036) ve MDA (malondialdehit) düzeyinin de ozon
tedavisi uygulanan grupta daha düşük olduğu, ancak istatistiksel olarak
anlamlılığa (p=0,093) ulaşmadığı görüldü. Histopatolojik açıdan da ozon grubu
ile kontrol grubunun benzer olduğu görüldü.



Sonuç: Ozon
tedavisi güçlü anti-inflamatuvar ve antioksidan etkinliği ile ekstermite
iskemi-reperfüzyon (IR) hasarını azaltmada alternatif bir tedavi seçeneği
olabilir.



Anahtar Kelimeler

Tavşanlar ozon femoral arter reperfüzyon hasarı iskemi antioksidanlar

Proje Numarası

2015-545

Kaynakça

  • 1. Turoczi Z, Aranyi P, Lukats A. Muscle Fiber Viability, A novel method for the fast detection of ischemic muscle injury in Rats PLoS One 2014;9(1):e84783 https://doi.org/10.1371/journal.pone.0084783
  • 2. Chen H1, Xing B, Liu X, Zhan B, Zhan B, Zhou J, et al. Ozone oxidative preconditioning inhibits inflammation and apoptosis in a rat model of renal ischemia/reperfusion injury. Eur J Pharmacol 2008;581(3):306-14. https://doi.org/10.1016/j.ejphar.2007.11.050
  • 3. Fernández I, González Núñez L, Calunga Fernández JL, Rodríguez Salgueiro S, Santos Febles E. Ozone postconditioning in renal ischaemia-reperfusion model. Functional and morphological evidences. Nefrologia 2011;31(4):464-70. https://doi.org/10.3265/Nefrologia.pre2011.May.10873
  • 4. Caliskan B, Guven A, Ozler M, Cayci T, Ozcan A, Bedir O, et al. Ozone therapy prevents renal inflammation and fibrosis in a rat model of acute pyelonephritis. Scand J Clin Lab Invest 2011;71(6):473-80. https://doi.org/10.3109/00365513.2011.587022
  • 5. Kumada M, Kihara S, Sumitsuji S, Kawamoto T, Matsumoto S, Ouchi N, et al. Association of hypoadiponectinemia with coronary artery disease in men. Arterioscler Thromb Vasc Biol 2003;23(1):85-9. https:// doi.org/10.1161/01.atv.0000048856.22331.50
  • 6. Ouchi N, Ohishi M, Kihara S, Funahashi T, NakamuraT, Nagaretani H, et al. Association of hypoadiponectinemia with impaired vasoreactivity. Hypertension 2003;42:231. https://doi.org/10.1161/01.HYP.0000083488.67550.B8
  • 7. Bulger EM, Cuschieri J, Warner K, Maier R. Hypertonic resuscitation modulates the inflammatory response in patients with traumatic hemorrhagic shock. Ann Surg 2007;245:635. https://doi.org/10.1097/01.sla.0000251367.44890.ae
  • 8. Kupiec-Weglinski JW, Busuttil RW. Ischemia and reperfusion injury in liver transplantation. Transpl Proc 2005;37:1653. https://doi.org/10.1016/j.transproceed.2005.03.134
  • 9. Granger DN, Kvietys PR, Perry MA. Leukocyte-endothelial cell adhesion induced by ischemia and reperfusion. Can J Physiol Pharmacol 1993;71:67–75. https://doi.org/10.1139/y93-011
  • 10. Kirschner RE, Fyfe BS, Hoffman LA, Chiao JJ, Davis JM, Fantini GA. Ischemia-reperfusion infury in myocutaneous flaps: role of leukocytes and leukotrienes. Plast Reconstr Surg 1997;99:1485–1493. https://www.ncbi.nlm.nih.gov/pubmed/9145114
  • 11. Cangemi R, Angelico F, Loffredo L, Del Ben M, Pignatelli P, Martini A, et al. Oxidative stress mediated arterial dysfunction in patients with metabolic syndrome: effect of ascorbic acid. Free Radic Biol Med 2007; 43:853. https://doi.org/10.1016/j.freeradbiomed.2007.06.002
  • 12. Webster NR, Nunn JF. Molecular structure of free radicals and their importance in biological reactions. Br J Anaesth 1988;60:98. https://doi.org/10.1093/bja/60.1.98
  • 13. Parabucki AB, Bozic ID, Bjelobaba IM, LavrnjaI, Brykc P, Jovanovic T, et al. Hyperbaric oxygenation alters temporal expression pattern of superoxide dismutase 2 after cortical stab injury in rats. Croat Med J 2012;53:586. https://doi.org/10.3325/cmj.2012.53.586
  • 14. Tural E, Uslu S, Ilgaz Aydinlar E, Irban A, Ince U, Orakdogen M, et al. Effects of ozone on spinal cord recovery via Wnt/ β-catenin pathway following spinal cord injury in rats. Turk Neurosurg 2017;27(6):946-54. https://doi.org/10.5137/1019-5149.JTN.17508-16.1
  • 15. Muto M, Giurazza F, Silva RP, Guarnieri G. Rational approach, technique and selection criteria treating lumbar disk herniations by oxygen-ozone therapy. Interv Neuroradiol 2016;22(6):736-40. https://doi.org/10.1177/1591019916659266
  • 16. Ergun Tasdoven G, Derin AT, Yaprak N, Ozcaglar HU. The place of hyperbaric oxygen therapy and ozone therapy in sudden hearing loss. Braz J Otorhinolaryngol 2017;83(4):457-63. https://doi.org/10.1016/j.bjorl.2016.06.002
  • 17. León Fernández OS, Viebahn-Haensler R, Cabreja GL, Espinosa IS, Matos YH, Roche LD, et al. Medical ozone increases methotrexate clinical response and improves cellular redox balance in patients with rheumatoid arthritis. Eur J Pharmacol 2016;789:313-8. https://doi.org/10.1016/j.ejphar.2016.07.031

The effects of ozone therapy on extremity ischemia-reperfusion injury in a rabbit model

Yıl 2019, Cilt: 4 Sayı: 3, 79 - 84, 31.12.2019

Burak Kaymaz , Ganime Coban , Ferdi Goksel , Huseyin Toman , Umut Hatay Golge , Erkam Komurcu

https://doi.org/10.22391/fppc.596783

Öz



Introduction: Ischemia is reversible or
irreversible cell/tissue damage that is secondary to insufficient blood flow to
tissues or organs. Ischemia causes many metabolic and structural changes at the
cellular level. Ozone therapy is often used as an antioxidant remedy. This
study aimed to investigate the effects of ozone therapy on extremity
ischemia-reperfusion (IR) injury.



Methods: Twenty-four New Zealand
White (NZW) rabbits were randomly allocated into three groups. Each group
consisted of eight rabbits. Group I was the control group, Group II was the
ischemia group, and Group III, the ozone group. 
Femoral arteries of the right legs were dissected, and femoral arterial
occlusion was performed in Group II and III. 
Hematological and histopathological evaluation was performed in all
groups.



Results: The levels of total
antioxidant status (TAS) after the surgical procedure was higher in the ozone
group compared to the ischemia group (p=0.036). In addition, the levels of
malondialdehyde (MDA) after the surgical procedure were lower in the ozone
group compared to the ischemia group but not statistically significant
(p=0.093). The control and ozone groups were histopathologically similar.



Conclusions: Ozone therapy may be used
as an alternative treatment modality with its anti-inflammatory and antioxidant
effects for the treatment of extremity IR injury.



Anahtar Kelimeler

Rabbits ozone femoral artery reperfusion injury ischemia antioxidants

Destekleyen Kurum

This work was supported by Research Fund of the Çanakkale Onsekiz Mart University

Proje Numarası

2015-545

Kaynakça

  • 1. Turoczi Z, Aranyi P, Lukats A. Muscle Fiber Viability, A novel method for the fast detection of ischemic muscle injury in Rats PLoS One 2014;9(1):e84783 https://doi.org/10.1371/journal.pone.0084783
  • 2. Chen H1, Xing B, Liu X, Zhan B, Zhan B, Zhou J, et al. Ozone oxidative preconditioning inhibits inflammation and apoptosis in a rat model of renal ischemia/reperfusion injury. Eur J Pharmacol 2008;581(3):306-14. https://doi.org/10.1016/j.ejphar.2007.11.050
  • 3. Fernández I, González Núñez L, Calunga Fernández JL, Rodríguez Salgueiro S, Santos Febles E. Ozone postconditioning in renal ischaemia-reperfusion model. Functional and morphological evidences. Nefrologia 2011;31(4):464-70. https://doi.org/10.3265/Nefrologia.pre2011.May.10873
  • 4. Caliskan B, Guven A, Ozler M, Cayci T, Ozcan A, Bedir O, et al. Ozone therapy prevents renal inflammation and fibrosis in a rat model of acute pyelonephritis. Scand J Clin Lab Invest 2011;71(6):473-80. https://doi.org/10.3109/00365513.2011.587022
  • 5. Kumada M, Kihara S, Sumitsuji S, Kawamoto T, Matsumoto S, Ouchi N, et al. Association of hypoadiponectinemia with coronary artery disease in men. Arterioscler Thromb Vasc Biol 2003;23(1):85-9. https:// doi.org/10.1161/01.atv.0000048856.22331.50
  • 6. Ouchi N, Ohishi M, Kihara S, Funahashi T, NakamuraT, Nagaretani H, et al. Association of hypoadiponectinemia with impaired vasoreactivity. Hypertension 2003;42:231. https://doi.org/10.1161/01.HYP.0000083488.67550.B8
  • 7. Bulger EM, Cuschieri J, Warner K, Maier R. Hypertonic resuscitation modulates the inflammatory response in patients with traumatic hemorrhagic shock. Ann Surg 2007;245:635. https://doi.org/10.1097/01.sla.0000251367.44890.ae
  • 8. Kupiec-Weglinski JW, Busuttil RW. Ischemia and reperfusion injury in liver transplantation. Transpl Proc 2005;37:1653. https://doi.org/10.1016/j.transproceed.2005.03.134
  • 9. Granger DN, Kvietys PR, Perry MA. Leukocyte-endothelial cell adhesion induced by ischemia and reperfusion. Can J Physiol Pharmacol 1993;71:67–75. https://doi.org/10.1139/y93-011
  • 10. Kirschner RE, Fyfe BS, Hoffman LA, Chiao JJ, Davis JM, Fantini GA. Ischemia-reperfusion infury in myocutaneous flaps: role of leukocytes and leukotrienes. Plast Reconstr Surg 1997;99:1485–1493. https://www.ncbi.nlm.nih.gov/pubmed/9145114
  • 11. Cangemi R, Angelico F, Loffredo L, Del Ben M, Pignatelli P, Martini A, et al. Oxidative stress mediated arterial dysfunction in patients with metabolic syndrome: effect of ascorbic acid. Free Radic Biol Med 2007; 43:853. https://doi.org/10.1016/j.freeradbiomed.2007.06.002
  • 12. Webster NR, Nunn JF. Molecular structure of free radicals and their importance in biological reactions. Br J Anaesth 1988;60:98. https://doi.org/10.1093/bja/60.1.98
  • 13. Parabucki AB, Bozic ID, Bjelobaba IM, LavrnjaI, Brykc P, Jovanovic T, et al. Hyperbaric oxygenation alters temporal expression pattern of superoxide dismutase 2 after cortical stab injury in rats. Croat Med J 2012;53:586. https://doi.org/10.3325/cmj.2012.53.586
  • 14. Tural E, Uslu S, Ilgaz Aydinlar E, Irban A, Ince U, Orakdogen M, et al. Effects of ozone on spinal cord recovery via Wnt/ β-catenin pathway following spinal cord injury in rats. Turk Neurosurg 2017;27(6):946-54. https://doi.org/10.5137/1019-5149.JTN.17508-16.1
  • 15. Muto M, Giurazza F, Silva RP, Guarnieri G. Rational approach, technique and selection criteria treating lumbar disk herniations by oxygen-ozone therapy. Interv Neuroradiol 2016;22(6):736-40. https://doi.org/10.1177/1591019916659266
  • 16. Ergun Tasdoven G, Derin AT, Yaprak N, Ozcaglar HU. The place of hyperbaric oxygen therapy and ozone therapy in sudden hearing loss. Braz J Otorhinolaryngol 2017;83(4):457-63. https://doi.org/10.1016/j.bjorl.2016.06.002
  • 17. León Fernández OS, Viebahn-Haensler R, Cabreja GL, Espinosa IS, Matos YH, Roche LD, et al. Medical ozone increases methotrexate clinical response and improves cellular redox balance in patients with rheumatoid arthritis. Eur J Pharmacol 2016;789:313-8. https://doi.org/10.1016/j.ejphar.2016.07.031
Toplam 17 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Cerrahi
Bölüm Araştırma Makalesi (Original Article)
Yazarlar

Burak Kaymaz 0000-0001-6207-8063

Ganime Coban 0000-0002-5779-6797

Ferdi Goksel 0000-0002-2672-2904

Huseyin Toman 0000-0003-2708-4163

Umut Hatay Golge 0000-0001-7384-6144

Erkam Komurcu 0000-0003-3358-0358

Proje Numarası 2015-545
Yayımlanma Tarihi 31 Aralık 2019
Gönderilme Tarihi 26 Temmuz 2019
Kabul Tarihi 24 Ekim 2019
Yayımlandığı Sayı Yıl 2019 Cilt: 4 Sayı: 3

Kaynak Göster

APA Kaymaz, B., Coban, G., Goksel, F., … Toman, H. (2019). The effects of ozone therapy on extremity ischemia-reperfusion injury in a rabbit model. Family Practice and Palliative Care, 4(3), 79-84. https://doi.org/10.22391/fppc.596783
AMA Kaymaz B, Coban G, Goksel F, Toman H, Golge UH, Komurcu E. The effects of ozone therapy on extremity ischemia-reperfusion injury in a rabbit model. Fam Pract Palliat Care. Aralık 2019;4(3):79-84. doi:10.22391/fppc.596783
Chicago Kaymaz, Burak, Ganime Coban, Ferdi Goksel, Huseyin Toman, Umut Hatay Golge, ve Erkam Komurcu. “The effects of ozone therapy on extremity ischemia-reperfusion injury in a rabbit model”. Family Practice and Palliative Care 4, sy. 3 (Aralık 2019): 79-84. https://doi.org/10.22391/fppc.596783.
EndNote Kaymaz B, Coban G, Goksel F, Toman H, Golge UH, Komurcu E (01 Aralık 2019) The effects of ozone therapy on extremity ischemia-reperfusion injury in a rabbit model. Family Practice and Palliative Care 4 3 79–84.
IEEE B. Kaymaz, G. Coban, F. Goksel, H. Toman, U. H. Golge, ve E. Komurcu, “The effects of ozone therapy on extremity ischemia-reperfusion injury in a rabbit model”, Fam Pract Palliat Care, c. 4, sy. 3, ss. 79–84, 2019, doi: 10.22391/fppc.596783.
ISNAD Kaymaz, Burak vd. “The effects of ozone therapy on extremity ischemia-reperfusion injury in a rabbit model”. Family Practice and Palliative Care 4/3 (Aralık2019), 79-84. https://doi.org/10.22391/fppc.596783.
JAMA Kaymaz B, Coban G, Goksel F, Toman H, Golge UH, Komurcu E. The effects of ozone therapy on extremity ischemia-reperfusion injury in a rabbit model. Fam Pract Palliat Care. 2019;4:79–84.
MLA Kaymaz, Burak vd. “The effects of ozone therapy on extremity ischemia-reperfusion injury in a rabbit model”. Family Practice and Palliative Care, c. 4, sy. 3, 2019, ss. 79-84, doi:10.22391/fppc.596783.
Vancouver Kaymaz B, Coban G, Goksel F, Toman H, Golge UH, Komurcu E. The effects of ozone therapy on extremity ischemia-reperfusion injury in a rabbit model. Fam Pract Palliat Care. 2019;4(3):79-84.

Family Practice and Palliative Care      ISSN 2458-8865       E-ISSN 2459-1505