Year 2019, Volume 4 , Issue 3, Pages 126 - 129 2019-12-31

The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1α) expression in rabbit cornea
Tavşan korneasında yüksek doz metilprednizolonun yanı sıra ozon (O3) tedavisinin hipoksi ile indüklenebilir faktör-1 alfa (HIF-la) ekspresyonu üzerine etkisi

Yusuf Haydar Ertekin [1] , Sedat Arıkan [2] , Ihsan Karaboga [3]


Introduction: To investigate the effect of ozone (O3) therapy on cornea subjected to systemically used high dose methylprednisolone (MP) in a rabbit model.

Methods:  Twenty-four New Zealand White adult male rabbits were randomly divided into three equal groups as containing eight animals. The first group (n = 8) was used as the control group and nothing was applied to them, whereas the other 2 groups named as steroid groups were subjected to IM methylprednisolone injection at a dose of 20 mg/kg/day for three days. After three days of MP administration, only the third group was treated with 50-µg/mL O3 (20 mL O3) through the rectal insufflation for 14 sessions. The histopathological examination of corneas of three groups were made, and they were also assessed regarding the expression of hypoxia-inducible factor-1 α (HIF-1α).

Results: It was determined that systemically administered high dose MP caused erosion and necrosis in corneal epithelium and stromal disintegrations in corneal stroma in steroid groups (Group 2 and Group 3). In the MP + O3 group (Group 3), the histopathological findings were mild. The expression of HIF1-α in the cornea of Group1 (control group), Group 2 (MP), and Group 3 (MP-O3) was measured as, 17.9±9.6%, 3.1±1.0% and 6.4±1.9% respectively.

Conclusions: MP and MP-O3 therapy decreased HIF-1a expression in rabbit cornea in both intervention groups. Between these two groups, HIF-1α expression remained relatively high in the MP-O3 group than in the MP group alone.

Giriş: Bir tavşan modelinde sistemik olarak kullanılan yüksek doz metilprednizolon (MP) uygulanmış kornea üzerinde ozon (O3) tedavisinin etkisini araştırmak.

Yöntem:  Yirmi dört Yeni Zelanda Beyaz yetişkin erkek tavşanı rastgele olarak sekiz hayvan içeren üç eşit gruba ayrıldı. Birinci grup (n = 8) kontrol grubu olarak kullanıldı ve onlara hiçbir şey uygulanmadı, diğer taraftan steroid grubu olarak adlandırılan diğer 2 grup, üç gün boyunca 20 mg / kg / gün dozunda IM metilprednizolon enjeksiyonuna tabi tutuldu. Üç günlük MP uygulamasından sonra, sadece üçüncü grup, 14 seans boyunca rektal insüflasyon yoluyla 50 ug / mL O3 (20 mL O3) ile tedavi edildi. Üç grubun kornealarının histopatolojik incelemesi yapıldı ve hipoksiye bağlı faktör-1 a (HIF-la) ekspresyonu açısından değerlendirildi.

Bulgular: Sistemik olarak uygulanan yüksek doz MP'nin steroid gruplarında kornea epitelinde erozyon ve nekroza ve korneal stromada stromal parçalanmaya neden olduğu belirlendi (Grup 2 ve Grup 3). MP + O3 grubunda (Grup 3) histopatolojik bulgular hafifti. HIF1-a'nın Grup 1 (kontrol grubu), Grup 2 (MP) ve Grup 3 (MP-O3) korneasında ekspresyonu sırasıyla %17.9 ± 9.6, % 3.1 ± 1.0 ve % 6.4 ± 1.9 olarak ölçüldü.

Sonuç: MP ve MP-O3 tedavisi, her iki müdahale grubunda da tavşan korneasında HIF-la ekspresyonunu azalttı. Bu iki grup arasında, HIF-la ekspresyonu MP-O3 grubunda sadece MP grubuna göre nispeten yüksek kaldı.

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Primary Language en
Subjects Medicine
Published Date Dec 2019
Journal Section Original Research
Authors

Orcid: 0000-0002-2016-9159
Author: Yusuf Haydar Ertekin (Primary Author)
Institution: Department of Family Medicine, School of Medicine, Canakkale Onsekiz Mart University, Canakkale
Country: Turkey


Orcid: 0000-0003-0541-6382
Author: Sedat Arıkan
Institution: Department of Ophthalmology, School of Medicine, Canakkale Onsekiz Mart University, Canakkale
Country: Turkey


Orcid: 0000-0001-6708-1229
Author: Ihsan Karaboga
Institution: Department of Emergency and Disaster Management, School of Health, Namik Kemal University, Tekirdag
Country: Turkey


Dates

Application Date : December 11, 2019
Acceptance Date : December 31, 2019
Publication Date : December 31, 2019

Bibtex @research article { fppc658034, journal = {Family Practice and Palliative Care}, issn = {2458-8865}, eissn = {2459-1505}, address = {}, publisher = {SERNEV}, year = {2019}, volume = {4}, pages = {126 - 129}, doi = {10.22391/fppc.658034}, title = {The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1α) expression in rabbit cornea}, key = {cite}, author = {Ertekin, Yusuf Haydar and Arıkan, Sedat and Karaboga, Ihsan} }
APA Ertekin, Y , Arıkan, S , Karaboga, I . (2019). The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1α) expression in rabbit cornea. Family Practice and Palliative Care , 4 (3) , 126-129 . DOI: 10.22391/fppc.658034
MLA Ertekin, Y , Arıkan, S , Karaboga, I . "The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1α) expression in rabbit cornea". Family Practice and Palliative Care 4 (2019 ): 126-129 <http://www.fppc.com.tr/en/issue/50274/658034>
Chicago Ertekin, Y , Arıkan, S , Karaboga, I . "The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1α) expression in rabbit cornea". Family Practice and Palliative Care 4 (2019 ): 126-129
RIS TY - JOUR T1 - The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1α) expression in rabbit cornea AU - Yusuf Haydar Ertekin , Sedat Arıkan , Ihsan Karaboga Y1 - 2019 PY - 2019 N1 - doi: 10.22391/fppc.658034 DO - 10.22391/fppc.658034 T2 - Family Practice and Palliative Care JF - Journal JO - JOR SP - 126 EP - 129 VL - 4 IS - 3 SN - 2458-8865-2459-1505 M3 - doi: 10.22391/fppc.658034 UR - https://doi.org/10.22391/fppc.658034 Y2 - 2019 ER -
EndNote %0 Family Practice and Palliative Care The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1α) expression in rabbit cornea %A Yusuf Haydar Ertekin , Sedat Arıkan , Ihsan Karaboga %T The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1α) expression in rabbit cornea %D 2019 %J Family Practice and Palliative Care %P 2458-8865-2459-1505 %V 4 %N 3 %R doi: 10.22391/fppc.658034 %U 10.22391/fppc.658034
ISNAD Ertekin, Yusuf Haydar , Arıkan, Sedat , Karaboga, Ihsan . "The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1α) expression in rabbit cornea". Family Practice and Palliative Care 4 / 3 (December 2020): 126-129 . https://doi.org/10.22391/fppc.658034
AMA Ertekin Y , Arıkan S , Karaboga I . The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1α) expression in rabbit cornea. Fam Pract Palliat Care. 2019; 4(3): 126-129.
Vancouver Ertekin Y , Arıkan S , Karaboga I . The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1α) expression in rabbit cornea. Family Practice and Palliative Care. 2019; 4(3): 129-126.