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Araştırma Makalesi
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Tavşan korneasında yüksek doz metilprednizolonun yanı sıra ozon (O3) tedavisinin hipoksi ile indüklenebilir faktör-1 alfa (HIF-la) ekspresyonu üzerine etkisi

Yıl 2019, Cilt: 4 Sayı: 3, 126 - 129, 31.12.2019

Yusuf Haydar Ertekin , Sedat Arıkan , İhsan Karaboga

https://doi.org/10.22391/fppc.658034
Cited By: 1

Öz



Giriş: Bir tavşan
modelinde sistemik olarak kullanılan yüksek doz metilprednizolon (MP)
uygulanmış kornea üzerinde ozon (O3) tedavisinin etkisini araştırmak.



Yöntem:  Yirmi dört Yeni Zelanda Beyaz yetişkin erkek
tavşanı rastgele olarak sekiz hayvan içeren üç eşit gruba ayrıldı. Birinci grup
(n = 8) kontrol grubu olarak kullanıldı ve onlara hiçbir şey uygulanmadı, diğer
taraftan steroid grubu olarak adlandırılan diğer 2 grup, üç gün boyunca 20 mg /
kg / gün dozunda IM metilprednizolon enjeksiyonuna tabi tutuldu. Üç günlük MP
uygulamasından sonra, sadece üçüncü grup, 14 seans boyunca rektal insüflasyon
yoluyla 50 ug / mL O3 (20 mL O3) ile tedavi edildi. Üç grubun kornealarının
histopatolojik incelemesi yapıldı ve hipoksiye bağlı faktör-1 a (HIF-la)
ekspresyonu açısından değerlendirildi.



Bulgular: Sistemik olarak
uygulanan yüksek doz MP'nin steroid gruplarında kornea epitelinde erozyon ve
nekroza ve korneal stromada stromal parçalanmaya neden olduğu belirlendi (Grup
2 ve Grup 3). MP + O3 grubunda (Grup 3) histopatolojik bulgular hafifti.
HIF1-a'nın Grup 1 (kontrol grubu), Grup 2 (MP) ve Grup 3 (MP-O3) korneasında
ekspresyonu sırasıyla %17.9 ± 9.6, % 3.1 ± 1.0 ve % 6.4 ± 1.9 olarak ölçüldü.



Sonuç: MP ve MP-O3
tedavisi, her iki müdahale grubunda da tavşan korneasında HIF-la ekspresyonunu
azalttı. Bu iki grup arasında, HIF-la ekspresyonu MP-O3 grubunda sadece MP
grubuna göre nispeten yüksek kaldı.



Anahtar Kelimeler

hipoksi ile indüklenebilir faktör 1 alfa subüniti tavşanlar ozon metilprednizolon kornea

Kaynakça

  • 1. Beck RW. The optic neuritis treatment trial. Implications for clinical practice. Optic Neuritis Study Group. Arch Ophthalmol 1992;110(3):331-2. https://doi.org/10.1001/archopht.1992.01080150029020
  • 2. Semenza GL. Targeting HIF-1 for cancer therapy. Nat Rev Cancer 2003;3(10):721-32. https://doi.org/10.1038/nrc1187
  • 3. Xia X, Kung AL. Preferential binding of HIF-1 to transcriptionally active loci determines cell-type specific response to hypoxia. Genome Biol 2009;10(10):R113. https://doi.org/10.1186/gb-2009-10-10-r113
  • 4. Eckle T, Köhler D, Lehmann R, El Kasmi K, Eltzschig HK. Hypoxia-inducible factor-1 is central to cardioprotection: a new paradigm for ischemic preconditioning. Circulation 2008;118(2):166-75. https://doi.org/10.1161/CIRCULATIONAHA.107.758516
  • 5. Semenza GL. Hypoxia-inducible factor 1: regulator of mitochondrial metabolism and mediator of ischemic preconditioning. Biochim Biophys Acta 2011;1813(7):1263-8. https://doi.org/10.1016/j.bbamcr.2010.08.006
  • 6. Ong SG, Lee WH, Theodorou L, Kodo K, Lim SY, Shukla DH, et al. HIF-1 reduces ischaemia-reperfusion injury in the heart by targeting the mitochondrial permeability transition pore. Cardiovasc Res 2014;104(1):24-36. https://doi.org/10.1093/cvr/cvu172
  • 7. Kucharz EJ. Hormonal control of collagen metabolism. Part II. Endocrinologie 1988;26(4):229-37. https://www.ncbi.nlm.nih.gov/pubmed/3062759
  • 8. Ryu JS, Ko JH, Kim MK, Wee WR, Oh JY. Prednisolone induces apoptosis in corneal epithelial cells through the intrinsic pathway. Sci Rep 2017;7(1):4135. https://doi.org/10.1038/s41598-017-04509-8
  • 9. Bourcier T, Forgez P, Borderie V, Scheer S, Rostène W, Laroche L. Regulation of human corneal epithelial cell proliferation and apoptosis by dexamethasone. Invest Ophthalmol Vis Sci 2000;41(13):4133–41. https://www.ncbi.nlm.nih.gov/pubmed/11095606
  • 10. Bourcier T, Borderie V, Forgez P, Lombet A, Rostène W, Laroche L. In vitro effects of dexamethasone on human corneal keratocytes. Invest Ophthalmol Vis Sci 1999;40(6):1061-70. https://www.ncbi.nlm.nih.gov/pubmed/10235539
  • 11. Yao L, Nie X, Shi S, Song S, Hao X, Li S, et al. Reciprocal regulation of HIF-1α and 15-LO/15-HETE promotes anti-apoptosis process in pulmonary artery smooth muscle cells during hypoxia. Prostaglandins Other Lipid Mediat 2012;99(3-4):96-106. https://doi.org/10.1016/j.prostaglandins.2012.09.001
  • 12. Vidal S, Horvath E, Kovacs K, Kuroki T, Lloyd RV, Scheithauer BW. Expression of hypoxia-inducible factor-1alpha (HIF-1alpha) in pituitary tumours. Histol Histopathol. 2003;18(3):679-86. https://doi.org/10.14670/HH-18.679
  • 13. Zhang C1, Qiang Q2, Jiang Y3, Hu L4, Ding X5, Lu Y6, Hu G7. Effects of hypoxia inducible factor-1α on apoptotic inhibition and glucocorticoid receptor downregulation by dexamethasone in AtT-20 cells. BMC Endocr Disord 2015;15:24. https://doi.org/10.1186/s12902-015-0017-2

The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1α) expression in rabbit cornea

Yıl 2019, Cilt: 4 Sayı: 3, 126 - 129, 31.12.2019

Yusuf Haydar Ertekin , Sedat Arıkan , İhsan Karaboga

https://doi.org/10.22391/fppc.658034
Cited By: 1

Öz

Introduction: To investigate the effect
of ozone (O3) therapy on cornea subjected to systemically used high dose
methylprednisolone (MP) in a rabbit model.



Methods:  Twenty-four New Zealand White adult male
rabbits were randomly divided into three equal groups as containing eight
animals. The first group (n = 8) was used as the control group and nothing was
applied to them, whereas the other 2 groups named as steroid groups were
subjected to IM methylprednisolone injection at a dose of 20 mg/kg/day for
three days. After three days of MP administration, only the third group was
treated with 50-µg/mL O3 (20 mL O3) through the rectal insufflation for 14
sessions. The histopathological examination of corneas of three groups were
made, and they were also assessed regarding the expression of hypoxia-inducible
factor-1 α (HIF-1α).



Results: It was determined that systemically
administered high dose MP caused erosion and necrosis in corneal epithelium and
stromal disintegrations in corneal stroma in steroid groups (Group 2 and Group
3). In the MP + O3 group (Group 3), the histopathological findings were mild.
The expression of HIF1-α in the cornea of Group1 (control group), Group 2 (MP),
and Group 3 (MP-O3) was measured as, 17.9±9.6%, 3.1±1.0% and 6.4±1.9% respectively.



Conclusions: MP and MP-O3 therapy decreased HIF-1a expression in rabbit cornea in
both intervention groups. Between these two groups, HIF-1α expression remained
relatively high in the MP-O3 group than in the MP group alone.

Anahtar Kelimeler

hypoxia inducible factor 1 alpha subunit rabbits ozone methylprednisolone cornea

Kaynakça

  • 1. Beck RW. The optic neuritis treatment trial. Implications for clinical practice. Optic Neuritis Study Group. Arch Ophthalmol 1992;110(3):331-2. https://doi.org/10.1001/archopht.1992.01080150029020
  • 2. Semenza GL. Targeting HIF-1 for cancer therapy. Nat Rev Cancer 2003;3(10):721-32. https://doi.org/10.1038/nrc1187
  • 3. Xia X, Kung AL. Preferential binding of HIF-1 to transcriptionally active loci determines cell-type specific response to hypoxia. Genome Biol 2009;10(10):R113. https://doi.org/10.1186/gb-2009-10-10-r113
  • 4. Eckle T, Köhler D, Lehmann R, El Kasmi K, Eltzschig HK. Hypoxia-inducible factor-1 is central to cardioprotection: a new paradigm for ischemic preconditioning. Circulation 2008;118(2):166-75. https://doi.org/10.1161/CIRCULATIONAHA.107.758516
  • 5. Semenza GL. Hypoxia-inducible factor 1: regulator of mitochondrial metabolism and mediator of ischemic preconditioning. Biochim Biophys Acta 2011;1813(7):1263-8. https://doi.org/10.1016/j.bbamcr.2010.08.006
  • 6. Ong SG, Lee WH, Theodorou L, Kodo K, Lim SY, Shukla DH, et al. HIF-1 reduces ischaemia-reperfusion injury in the heart by targeting the mitochondrial permeability transition pore. Cardiovasc Res 2014;104(1):24-36. https://doi.org/10.1093/cvr/cvu172
  • 7. Kucharz EJ. Hormonal control of collagen metabolism. Part II. Endocrinologie 1988;26(4):229-37. https://www.ncbi.nlm.nih.gov/pubmed/3062759
  • 8. Ryu JS, Ko JH, Kim MK, Wee WR, Oh JY. Prednisolone induces apoptosis in corneal epithelial cells through the intrinsic pathway. Sci Rep 2017;7(1):4135. https://doi.org/10.1038/s41598-017-04509-8
  • 9. Bourcier T, Forgez P, Borderie V, Scheer S, Rostène W, Laroche L. Regulation of human corneal epithelial cell proliferation and apoptosis by dexamethasone. Invest Ophthalmol Vis Sci 2000;41(13):4133–41. https://www.ncbi.nlm.nih.gov/pubmed/11095606
  • 10. Bourcier T, Borderie V, Forgez P, Lombet A, Rostène W, Laroche L. In vitro effects of dexamethasone on human corneal keratocytes. Invest Ophthalmol Vis Sci 1999;40(6):1061-70. https://www.ncbi.nlm.nih.gov/pubmed/10235539
  • 11. Yao L, Nie X, Shi S, Song S, Hao X, Li S, et al. Reciprocal regulation of HIF-1α and 15-LO/15-HETE promotes anti-apoptosis process in pulmonary artery smooth muscle cells during hypoxia. Prostaglandins Other Lipid Mediat 2012;99(3-4):96-106. https://doi.org/10.1016/j.prostaglandins.2012.09.001
  • 12. Vidal S, Horvath E, Kovacs K, Kuroki T, Lloyd RV, Scheithauer BW. Expression of hypoxia-inducible factor-1alpha (HIF-1alpha) in pituitary tumours. Histol Histopathol. 2003;18(3):679-86. https://doi.org/10.14670/HH-18.679
  • 13. Zhang C1, Qiang Q2, Jiang Y3, Hu L4, Ding X5, Lu Y6, Hu G7. Effects of hypoxia inducible factor-1α on apoptotic inhibition and glucocorticoid receptor downregulation by dexamethasone in AtT-20 cells. BMC Endocr Disord 2015;15:24. https://doi.org/10.1186/s12902-015-0017-2
Toplam 13 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Klinik Tıp Bilimleri
Bölüm Araştırma Makalesi (Original Article)
Yazarlar

Yusuf Haydar Ertekin 0000-0002-2016-9159

Sedat Arıkan 0000-0003-0541-6382

İhsan Karaboga 0000-0001-6708-1229

Yayımlanma Tarihi 31 Aralık 2019
Gönderilme Tarihi 11 Aralık 2019
Kabul Tarihi 31 Aralık 2019
Yayımlandığı Sayı Yıl 2019 Cilt: 4 Sayı: 3

Kaynak Göster

APA Ertekin, Y. H., Arıkan, S., & Karaboga, İ. (2019). The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1α) expression in rabbit cornea. Family Practice and Palliative Care, 4(3), 126-129. https://doi.org/10.22391/fppc.658034
AMA Ertekin YH, Arıkan S, Karaboga İ. The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1α) expression in rabbit cornea. Fam Pract Palliat Care. Aralık 2019;4(3):126-129. doi:10.22391/fppc.658034
Chicago Ertekin, Yusuf Haydar, Sedat Arıkan, ve İhsan Karaboga. “The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1α) expression in rabbit cornea”. Family Practice and Palliative Care 4, sy. 3 (Aralık 2019): 126-29. https://doi.org/10.22391/fppc.658034.
EndNote Ertekin YH, Arıkan S, Karaboga İ (01 Aralık 2019) The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1α) expression in rabbit cornea. Family Practice and Palliative Care 4 3 126–129.
IEEE Y. H. Ertekin, S. Arıkan, ve İ. Karaboga, “The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1α) expression in rabbit cornea”, Fam Pract Palliat Care, c. 4, sy. 3, ss. 126–129, 2019, doi: 10.22391/fppc.658034.
ISNAD Ertekin, Yusuf Haydar vd. “The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1α) expression in rabbit cornea”. Family Practice and Palliative Care 4/3 (Aralık2019), 126-129. https://doi.org/10.22391/fppc.658034.
JAMA Ertekin YH, Arıkan S, Karaboga İ. The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1α) expression in rabbit cornea. Fam Pract Palliat Care. 2019;4:126–129.
MLA Ertekin, Yusuf Haydar vd. “The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1α) expression in rabbit cornea”. Family Practice and Palliative Care, c. 4, sy. 3, 2019, ss. 126-9, doi:10.22391/fppc.658034.
Vancouver Ertekin YH, Arıkan S, Karaboga İ. The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1α) expression in rabbit cornea. Fam Pract Palliat Care. 2019;4(3):126-9.

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Aydın Yıldız
https://doi.org/10.1080/15569527.2020.1790590

Family Practice and Palliative Care      ISSN 2458-8865       E-ISSN 2459-1505